Results 71 to 80 of about 497,526 (307)
A Modular Cloning Toolkit for Genome Editing in Cereals
, 2022 The CRISPR/Cas technology has recently become the tool of choice for gene editing applications in plants.Here we describe the method of assembling CRISPR/Cas DNA constructs, using the modular cloning (MoClo), for deployment in cereal species.
Hahn, F. +2 more
core +1 more source
Plant Methods, 2011 Background Cloning genes into plasmid vectors is one of the key steps for studying gene function. Recently, Invitrogen™ developed a convenient Gateway® cloning system based on the site-specific DNA recombination properties of bacteriophage lambda and the
Traore Sy, Zhao Bingyu
doaj +1 more source
Tumour–host interactions in Drosophila: mechanisms in the tumour micro‐ and macroenvironment
Molecular Oncology, EarlyView.This review examines how tumour–host crosstalk takes place at multiple levels of biological organisation, from local cell competition and immune crosstalk to organism‐wide metabolic and physiological collapse. Here, we integrate findings from Drosophila melanogaster studies that reveal conserved mechanisms through which tumours hijack host systems to ...
José Teles‐Reis, Tor Erik Rusten
wiley +1 more source
Cloning of the Complete Gene for Carcinoembryonic Antigen [PDF]
, 1990 Carcinoembryonic antigen (CEA) is a widely used tumor marker, especially in the surveillance of colonic cancer patients. Although CEA is also present in some normal tissues, it is apparently expressed at higher levels in tumorous tissues than in ...
Schrewe, Heinrich +8 more
core
Construction and application of an insertional activation cloning vector
BMC BiotechnologyBackground Foreign DNA cloning is a critical step in genetic engineering and molecular biology for acquiring target genes. Efficient and accurate identification of positive clones from transformants is essential to this process.
Yan Zhang +4 more
doaj +1 more source
Rapid gene cloning in cereals [PDF]
Theoretical and Applied Genetics, 2018 The large and complex genomes of many cereals hindered cloning efforts in the past. Advances in genomics now allow the rapid cloning of genes from humanity's most valuable crops. The past two decades were characterized by a genomics revolution that entailed profound changes to crop research, plant breeding, and agriculture.
Bettgenhaeuser, Jan +1 more
openaire +3 more sources
Molecular Oncology, EarlyView.In this explorative study, the abundance of circular RNA molecules in bone marrow stem cells was found to be elevated in patients with high‐risk myelodysplastic neoplasms, and to be associated with an increased risk of progression to acute myeloid leukemia.
Eileen Wedge +17 more
wiley +1 more source
The Carcinoembryonic Antigen Gene Family [PDF]
, 1988 The molecular cloning of carcinoembryonic antigen (CEA) and several cross-reacting antigens reveals a basic domain structure for the whole family, which shows structural similarities to the immunoglobulin superfamily.
Zimmermann, Wolfgang, Thompson, John A.
core
COMP–PMEPA1 axis promotes epithelial‐to‐mesenchymal transition in breast cancer cells
Molecular Oncology, EarlyView.This study reveals that cartilage oligomeric matrix protein (COMP) promotes epithelial‐to‐mesenchymal transition (EMT) in breast cancer. We identify PMEPA1 (protein TMEPAI) as a novel COMP‐binding partner that mediates EMT via binding to the TSP domains of COMP, establishing the COMP–PMEPA1 axis as a key EMT driver in breast cancer.
Konstantinos S. Papadakos +6 more
wiley +1 more source
Molecular cloning and expression analysis of a new stress-related AREB gene from Arachis hypogaea
, 2013 An AREB gene, designated as AhAREB1, was cloned from peanut (Arachis hypogaea L.). The gene contains a 1 338-bp open reading frame that encodes a putative protein of 445 amino acids.
L. Hong +6 more
core +1 more source