Results 91 to 100 of about 480,078 (261)

Cloning of Dense Granular (GRA) 7 Gene of Toxoplasma gondii into pTZ57RT Vectors for Sub-Cloning in Prokaryotic and Eukaryotic Plasmids

Novelty in Biomedicine, 2014
Background: Serological assay based on dense granular (GRA) proteins of Toxoplasma gondii (T. gondii) is actually the most popular laboratory diagnostic tool to detection of toxoplasmosis.
Zahra Arab-Mazar, Seyed Javad Seyyed-Tabaei, Hadi Mirahmadi   +2 more
doaj  

ADH Gene Cloning and Identification of Flooding-Responsive Genes in Taxodium distichum (L.) Rich. [PDF]

Plants (Basel), 2023
Zhang R, Xuan L, Ni L, Yang Y, Zhang Y, Wang Z, Yin Y, Hua J.   +7 more
europepmc   +1 more source

Somatostatin receptor 4 (SSTR4) is a tumor suppressor in cutaneous and head & neck squamous cell carcinomas

Molecular Oncology, EarlyView.
This study identifies somatostatin receptor 4 (Sstr4) as a critical tumor suppressor against skin and head/neck cancers (HNSCC, cSCC, and BCC). The loss of Sstr4 removes a check on cell growth, causing hyperactivation of the MAPK‐ERK signaling pathway (↑).
Ali Taqvi, Tuan Khang Huynh, Adway Kadam, Zahra Kardan, Yue Yan, Amina Jamal Laham, Sampath Kumar Loganathan   +6 more
wiley   +1 more source

Cloning and Expression of Toxoplasma gondii Rhoptry13 Gene in pcDNA3 Plasmid

Journal of Mazandaran University of Medical Sciences, 2019
Background and purpose: Toxoplasma gondii (T. gondii) is an obligatory intracellular protozoan parasite. Toxoplasmosis is highly prevalent and has a great effect on public health, therefor, there is a need for vaccine and sensitive diagnostic procedures.
Paria Alizadeh, Ahamd Daryani, Ehsan Ahmadpour, Tohid Kazemi, Adel Spotin, Mahmoud Mahami-Oskoui, Yaghob Azadi, Saba Rajabi, Dariush Shanehbandi   +8 more
doaj  

Gene cloning and molecular characterization of a thermostable chitosanase from Bacillus cereus TY24. [PDF]

BMC Biotechnol, 2022
Zhang RX, Wu ZW, Zhang SJ, Wei HM, Hua CW, Li L, Yang TY.   +6 more
europepmc   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

Rice Lesion Mimic Gene Cloning and Association Analysis for Disease Resistance. [PDF]

Curr Issues Mol Biol, 2022
Zhang A, Jiang H, Chu H, Cao L, Chen J.
europepmc   +1 more source

YIPFα1A expression is regulated by multilayered molecular mechanisms

FEBS Open Bio, EarlyView.
YIPFα1A, a five‐pass Golgi protein, is regulated at multiple layers. (1) Rare‐codon enrichment drives translation‐coupled mRNA decay. (2) A proximal 3′‐UTR element stabilizes mRNA. (3) A distal 3′‐UTR element included by alternate poly(A) site usage represses translation, which can be overridden by the proximal 3′‐UTR element.
Tokio Takaji, Yurika Nakanishi, Nobuhiro Nakamura   +2 more
wiley   +1 more source

Toxin gene cloning [PDF]

Nature, 1983
openaire   +2 more sources

In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly. [PDF]

STAR Protoc, 2022
Wolff A, Wagner C, Wolf J, Lobo D.
europepmc   +1 more source