Results 251 to 260 of about 20,871,284 (295)
Some of the next articles are maybe not open access.
Delivery systems for gene therapy
Biotherapy, 1991Introduction of foreign genes into mammalian cells in vitro has been accomplished previously by a variety of methods. The few techniques that have been developed for transfection of mammalian cells in vivo, are technically difficult or lack cell specificity.
G Y, Wu, C H, Wu
openaire +2 more sources
Bioinspired and Biomimetic Gene Delivery Systems
ACS Applied Bio Materials, 2023Gene therapy that can introduce, counteract, or replace genes possesses great potential to address diseases at their genetic roots. A wide range of technologies, such as RNA interference, genome editing, DNA transformation, and mRNA vaccines, have been extensively investigated to modulate gene expression in an attempt to treat a myriad of diseases ...
Mong Tuyen Ho +3 more
openaire +2 more sources
2005
Polymeric gene delivery systems have been developed to overcome problems caused by viral carriers. They are low cytotoxic, have no size limit, are convenient in handling, of low cost and reproducible. A Terplex gene delivery system consisting of plasmid DNA, low density lipoprotein and hydropholized poly-L-lysine was designed and characterized.
openaire +3 more sources
Polymeric gene delivery systems have been developed to overcome problems caused by viral carriers. They are low cytotoxic, have no size limit, are convenient in handling, of low cost and reproducible. A Terplex gene delivery system consisting of plasmid DNA, low density lipoprotein and hydropholized poly-L-lysine was designed and characterized.
openaire +3 more sources
Rationale for the Selection of an Aerosol Delivery System for Gene Delivery
Journal of Aerosol Medicine, 2006Genetic therapeutics show great promise toward the treatment of illnesses associated with the lungs; however, current methods of delivery such as jet and ultrasonic nebulization decrease the activity and effectiveness of these treatments. Extremely low transfection rates exhibited by non-complexed plasmid DNA in these nebulizers have been primarily ...
Yvonne K, Lentz +2 more
openaire +2 more sources
Bacterial Systems for Gene Delivery to Systemic Tumors
2014Certain bacteria have emerged as biological gene vectors with natural tumor specificity, capable of specifically delivering genes or gene products to the tumor environment when intravenously (i.v.) administered to rodent models. Here, we describe procedures for studying this phenomenon in vitro and in vivo for both invasive and noninvasive bacteria ...
Joanne, Cummins +4 more
openaire +2 more sources
CPP-Based Delivery System for In Vivo Gene Delivery
2015The method presents most important steps in estimating a CPP-mediated reporter gene delivery in a mouse model. The method is applicable for administrating noncovalent CPP/pDNA complexes via i.v. injection and for analysis of luciferase levels in tissue homogenates.
Kaido, Kurrikoff +2 more
openaire +2 more sources
Nanoparticulated Polymeric Systems for Gene Delivery
Current Pharmaceutical Design, 2015Gene therapy, currently, represents one of the new therapies that have emerged for the treatment of different diseases. Between the different approaches that can be chosen concerning this therapy, gene delivery with non-viral systems has focused the attention during the past decades, because of the reduced toxicity compared to the viral systems.
Koldo, Urbiola +2 more
openaire +2 more sources
Synthetic Sustained Gene Delivery Systems
Current Topics in Medicinal Chemistry, 2008Gene therapy today is hampered by the need of a safe and efficient gene delivery system that can provide a sustained therapeutic effect without cytotoxicity or unwanted immune responses. Bolus gene delivery in solution results in the loss of delivered factors via lymphatic system and may cause undesired effects by the escape of bioactive molecules to ...
Ankit, Agarwal, Surya K, Mallapragada
openaire +2 more sources
Gene therapy and DNA delivery systems
International Journal of Pharmaceutics, 2014Gene therapy is a promising new technique for treating many serious incurable diseases, such as cancer and genetic disorders. The main problem limiting the application of this strategy in vivo is the difficulty of transporting large, fragile and negatively charged molecules like DNA into the nucleus of the cell without degradation.
Ibraheem, D., Elaissari, A., Fessi, H.
openaire +2 more sources
Gene Therapy and Gene Delivery Systems
20051 D.V. Schaffer, W. Zhou: Gene Therapy and Gene Delivery Systems as Future Human Therapeutics.- 2 J. Heidel, S. Mishra, M.E. Davis: Molecular Conjugates.- 3 M. Manthorpe, P. Hobart, G. Hermanson, M. Ferrari, A. Geall, B. Goff, A. Rolland: Plasmid Vaccines and Therapeutics: From Design to Applications.- 4 S.R. Little, R.
openaire +1 more source