Results 71 to 80 of about 189,147 (282)

Integrating malaria vaccine and CRISPR/Cas9 gene drive: a comprehensive strategy for accelerated malaria eradication

Malaria Journal
Malaria remains a significant public health challenge, particularly in low- and middle-income countries, despite ongoing efforts to eradicate the disease. Recent advancements, including the rollout of malaria vaccines, such as RTS,S/AS01 and R21/Matrix-M™
Israel Charles Abraham, John Ehi Aboje, Bonaventure Michael Ukoaka, Kehinde Tom-Ayegunle, Maryam Amjad, Anas Abdulkader, Chinonyelum Emmanuel Agbo, Oluwatosin Ayokunle Akinruli, Taiwo Rebecca Akisanmi, Emmanuel Oyedeji Oyetola, Gbolahan Olatunji, Emmanuel Kokori, Nicholas Aderinto   +12 more
doaj   +1 more source

Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae

Fungal Biology and Biotechnology, 2019
Background The bacterial CRISPR/Cas genome editing system has provided a major breakthrough in molecular biology. One use of this technology is within a nuclease-based gene drive.
Megan E. Goeckel, Erianna M. Basgall, Isabel C. Lewis, Samantha C. Goetting, Yao Yan, Megan Halloran, Gregory C. Finnigan   +6 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Repeat mediated excision of gene drive elements for restoring wild-type populations.

PLoS Genetics
Here, we demonstrate that single strand annealing (SSA) can be co-opted for the precise autocatalytic excision of a drive element. We have termed this technology Repeat Mediated Excision of a Drive Element (ReMEDE). By engineering direct repeats flanking
Pratima R Chennuri, Josef Zapletal, Raquel D Monfardini, Martial Loth Ndeffo-Mbah, Zach N Adelman, Kevin M Myles   +5 more
doaj   +1 more source

Modeling confinement and reversibility of threshold-dependent gene drive systems in spatially-explicit Aedes aegypti populations

BMC Biology, 2020
Background The discovery of CRISPR-based gene editing and its application to homing-based gene drive systems has been greeted with excitement, for its potential to control mosquito-borne diseases on a wide scale, and concern, for the invasiveness and ...
Héctor M. Sánchez C., Jared B. Bennett, Sean L. Wu, Gordana Rašić, Omar S. Akbari, John M. Marshall   +5 more
doaj   +1 more source

Aggressive prostate cancer is associated with pericyte dysfunction

Molecular Oncology, EarlyView.
Tumor‐produced TGF‐β drives pericyte dysfunction in prostate cancer. This dysfunction is characterized by downregulation of some canonical pericyte markers (i.e., DES, CSPG4, and ACTA2) while maintaining the expression of others (i.e., PDGFRB, NOTCH3, and RGS5).
Anabel Martinez‐Romero, Ane Martinez‐Larrinaga, Joaquim Grego‐Bessa, Saioa Garcia‐Longarte, Hielke van Splunder, Ianire Astobiza, Amaia Ercilla, Laura Bozal‐Basterra, Isabel Mendizabal, Pilar Villacampa, Arkaitz Carracedo, Mariona Graupera   +11 more
wiley   +1 more source

North American red fox rabies immunity gene drive for safer (sub)urban rewilding [PDF]

Research Ideas and Outcomes
Animal-transmitted diseases such as rabies represent a barrier to successful rewilding and threaten continued human-wildlife co-existence. In North America, population growth and human settlement expansion lead to encounters with wild mammals which have ...
Vixey Douglas
doaj   +3 more sources

Modelling the suppression of a malaria vector using a CRISPR-Cas9 gene drive to reduce female fertility

BMC Biology, 2020
Background Gene drives based on CRISPR-Cas9 technology are increasingly being considered as tools for reducing the capacity of mosquito populations to transmit malaria, and one of the most promising options is driving endonuclease genes that reduce the ...
Ace R. North, Austin Burt, H. Charles J. Godfray   +2 more
doaj   +1 more source

Gene drive technology as human intervention into nature : On the fate of environmental ethics in the anthropocene

Keje Boersma
openalex   +3 more sources