A Brassica exon array for whole-transcript gene expression profiling [PDF]
Affymetrix GeneChip® arrays are used widely to study transcriptional changes in response to developmental and environmental stimuli. GeneChip® arrays comprise multiple 25-mer oligonucleotide probes per gene and retain certain advantages over direct ...
King Graham J. +54 more
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Exon and junction microarrays detect widespread mouse strain- and sex-bias expression differences
Background Studies have shown that genetic and sex differences strongly influence gene expression in mice. Given the diversity and complexity of transcripts produced by alternative splicing, we sought to use microarrays to establish the extent of ...
Schadt Eric E +9 more
doaj +1 more source
Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling [PDF]
Vijayendran C, Barsch A, Friehs K, Niehaus K, Becker A, Flaschel E. Perceiving molecular evolution processes in Escherichia coli by comprehensive metabolite and gene expression profiling. GENOME BIOLOGY.
Flaschel, Erwin +5 more
core +1 more source
Gene expression analysis in microdissected renal tissue - Current challenges and strategies [PDF]
The architecture and compartmentalization of the kidney has stimulated the development of an array of microtechniques to study the functional differences between the distinct nephron segments. With the vast amounts of genomic sequence data now available,
Cohen, C. D., Kretzler, M.
core +1 more source
Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]
Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Wagner, Carola +4 more
core +1 more source
Use of homologous and heterologous gene expression profiling tools to characterize transcription dynamics during apple fruit maturation and ripening [PDF]
Background Fruit development, maturation and ripening consists of a complex series of biochemical and physiological changes that in climacteric fruits, including apple and tomato, are coordinated by the gaseous hormone ethylene.
Valeria Soglio +56 more
core +1 more source
Gene expression profiling measures the relative abundance of a large number of individual mRNA species within the context of a total mRNA population that has been isolated and purified from a target biological sample. The technology for achieving this evolved from low-Throughput membrane-based probeto- target hybridization assays (northern and Southern
C. Sotiriou, P. Dinh
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Gene expression profiling en association with prion-related lesions in the medulla oblongata of symptomatic natural scrapie animals. [PDF]
The pathogenesis of natural scrapie and other prion diseases remains unclear. Examining transcriptome variations in infected versus control animals may highlight new genes potentially involved in some of the molecular mechanisms of prion-induced ...
Bossers, A. +34 more
core +2 more sources
High-throughput SuperSAGE for digital gene expression analysis of multiple samples using next generation sequencing [PDF]
We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined "High- Throughput (HT-) SuperSAGE". SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from ...
Schroth, G.P. +68 more
core +1 more source
Gene Expression Profiling of Xeroderma Pigmentosum
Xeroderma pigmentosum (XP) is a rare recessive disorder that is characterized by extreme sensitivity to UV light. UV light exposure results in the formation of DNA damage such as cyclobutane dimers and (6-4) photoproducts. Nucleotide excision repair (NER)
Bowden Nikola A +2 more
doaj +1 more source

