Results 181 to 190 of about 423,637 (219)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

The HOXA gene cluster: a critical regulator in bone-related disorders. [PDF]

Ann Med
Zhang Y, Zhang L, Li Y, Bai J, Lv H.
europepmc   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

How Epigenetics Bridges Genes and Environment: A Multiomics Evidence Chain in Congenital Diaphragmatic Hernia. [PDF]

Birth Defects Res
Gao Y, Zhu X, Dai R, Zhang X, Ma Y, Dao Y, Chen J, Yang S, Wu J.   +8 more
europepmc   +1 more source

Aging Is a Key Driver for Adult Acute Myeloid Leukemia

Aging and Cancer, EarlyView.
Acute myeloid leukemia (AML) is a classical age‐related hematologic malignancy, and a key driver of AML is aging, which profoundly regulates intrinsic factors such as genomic instability, epigenetic reprogramming, and metabolic dysregulation, and alters bone marrow microenvironment.
Rong Yin, Haojian Zhang
wiley   +1 more source

The regulatory role of m⁶A RNA methylation in insect sexual development: a review. [PDF]

Front Cell Dev Biol
Duan T, Jin B, Xu Y, Xu J, Cen B.
europepmc   +1 more source

Stage-specific regulation of soybean stem growth by exogenous gibberellin (GA₃). [PDF]

BMC Plant Biol
Shan F, Wang C, Lyu X, Ma C, Gong Z.
europepmc   +1 more source

RNA variation as the driver of genomic efficiency and phenotypic complexity. [PDF]

Int J Biol Sci
Jiang Z, van Rooyen M, Michal JJ, Khaerunnisa I, Anwar S, Fu X, Yang J, Miller SP.   +7 more
europepmc   +1 more source

Editorial: Chromatin modifications and gene expression: from mechanisms to therapeutic implications in disease. [PDF]

Front Genet
Burlibasa L, Nassa G, Botezatu A, Salvati A.   +3 more
europepmc   +1 more source