Results 191 to 200 of about 1,333,192 (304)

HighALPS: ultra-high-throughput marker-gene amplicon library preparation and sequencing on the Illumina NextSeq and NovaSeq Platforms. [PDF]

mSystems
Flörl L, Cabrera PM, Moccia MD, Plüss S, Bokulich NA.   +4 more
europepmc   +1 more source

Microengineered Gradient Hydrogels for Mechanobiology

Advanced Healthcare Materials, EarlyView.
Gradient hydrogels are used to mimic the mechanical heterogeneity in native tissues, offering powerful in vitro platforms to study cell‐material interactions in diverse pathophysiological contexts. Here, we present a comprehensive review of the design and experimental considerations for stiffness gradient hydrogels, discussing exemplary achievements ...
Shin Wei Chong, Deepu Ashok, Anna Waterhouse, Marcela M. M. Bilek, Daniele Vigolo   +4 more
wiley   +1 more source

Protocol to identify SINE-VNTR-Alu regulators using genome-wide screening in human K562 cells. [PDF]

STAR Protoc
Zhou Z, Zhu S, Pan D, Lee HY, Liu N.
europepmc   +1 more source

A Data Driven Review of In Vitro Electrical and Mechanical Stimulation for Post‐Acute Phase Wound Healing

Advanced Healthcare Materials, EarlyView.
This review examines how in vitro electrical and mechanical stimulation modulates wound healing in fibroblasts and keratinocytes. Analyzing over 560 experimental data points, we relate stimulation parameters to proliferation and migration outcomes, evaluate platform designs, and highlight the need for multi‐parameter optimization to advance targeted ...
Matthew K. Burgess, Ellen F. Marsh, Veronica M. Lucian, Malavika Nair   +3 more
wiley   +1 more source

Targeting redundant gene families: A multiplexed, tissue-specific CRISPR toolbox for Arabidopsis genetic screens.

Cell Rep
Anfang M, Yahya RH, Caldararu O, Ben Yaakov S, Landau U, Berman A, Hu Y, Belew ZM, Crocoll C, Xu D, Nour-Eldin HH, Mayrose I, Shani E.   +12 more
europepmc   +1 more source

Peptide ligase-mediated display: A cell-free platform for tunable selection of affinity peptides. [PDF]

PNAS Nexus
Ueno S, Toshioka F, Ichiki T.
europepmc   +1 more source

Plasmonic Enhancement of Fluorescence and Protein Dynamics in Living Mammalian Cells

Advanced Materials, EarlyView.
This study demonstrates plasmonic enhancement of the function of fluorescent voltage sensing proteins (genetically encoded voltage indicators, (GEVIs), QuasAr6) in live mammalian cells. Coupling to plasmonic nanoparticles does not just increase fluorescence, but influences the protein photocycle, creating a hybrid sensor with its response speed to ...
Marco Locarno, Qiangrui Dong, Marco Post, Xin Meng, Cristiano Glessi, Nynke Marije Hettema, Nidas Brandsma, Sebbe Blokhuizen, Alejandro Castañeda Garcia, Srividya Ganapathy, Thieme Schmidt, Lars van Roemburg, Bing Xu, Chun‐Ting Cho, Liedewij Laan, Miao‐Ping Chien, Daan Brinks   +16 more
wiley   +1 more source

Deep-mutational scanning libraries using Tiled-Region Exchange mutagenesis. [PDF]

G3 (Bethesda)
Kindree K, Chochinov CA, Bhachu K, Cheng Y, Caron A, McDonald M, Mamai Z, Nguyen Ba AN.   +7 more
europepmc   +1 more source

A Cryoprotectant‐Compatible Nanoporous Platform for Stable and Scalable Delivery of Biopharmaceuticals

Advanced Materials, EarlyView.
A cryoprotectant‐compatible nanoporous platform enables ambient‐stable and scalable delivery of gene editing therapeutics. By combining hierarchical pore architecture with optimized lyophilization chemistry, the system preserves Cas9‐RNP activity post‐freeze‐drying.
Sian Lee, Seongchan Kim, Jooyeun Chong, Min‐gyeong Kim, Kayeong Lim, Nan‐Ee Lee, Jahyun Koo, Jiheong Kang, Hyojin Lee   +8 more
wiley   +1 more source

Directed evolution of novel AAV capsids for enhanced delivery to mouse and human Schwann cells. [PDF]

Mol Ther Nucleic Acids
Carneiro AD, Nisanov AM, Lee H, Schaffer DV.   +3 more
europepmc   +1 more source