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Methicillin-resistant Staphylococcus aureus (MRSA) is the main cause of hospital infection emerged over the last decades. Rapid detection of MRSA is important for patient care and proper usage of infection control.
Roxana Sahebnasagh +2 more
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Identification of mecA gene in MRSA by multiplex PCR.
HARA, Sachiko +6 more
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Analysis of the mecA Gene Conferring Methicillin Resistance in Staphylococcus aureus
This study presents an in-silico analysis of the MecA drug resistance gene from Staphylococcus aureus, the key determinant of methicillin resistance. The complete gene sequence (1,800 bp; GC% = 33.67) was retrieved from NCBI and translated using Expasy tools, resulting in a 600-amino-acid protein with a molecular weight of 76.1 kDa and a theoretical ...
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Detection of enterotoxigenic genes and mecA gene in Staphylococcus aureus isolated from goat milk
Germana Guimarães Rebouças +5 more
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Ratio of mecA gene in oxacillin-insusceptible and susceptible Staphylococcus aureus
Wada, Akiko +6 more
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Assessment of oxacillin salt agar for detection of MRSA identified by presence of the mecA gene
Journal of Hospital Infection, 1993To assess the screening method for detection of methicillin-resistant Staphylococcus aureus recommended by Thornsberry and McDougal J. Clin Microbiol 1983; 18: 1084-1901, the growth of S. aureus and Staphylococcus epidermidis strains on Mueller Hinton agar containing 4% NaCl and 6 mg l-1 oxacillin, after 24 h incubation at 35 degrees C, was ...
M Kizaki
exaly +3 more sources
Introduction: Staphylococci are common human commensals that acquire methicillin resistance via the mecA gene. Methicillin resistance in staphylococci from various clinical sources has been assessed using cefoxitin disc diffusion test (CDDT) and PCR ...
Jams Jayatilake
exaly +3 more sources
Surveillance of variant mecA gene and analysis of efflux pump in mecA negative MRSA infections
Pakistan Journal of Pharmaceutical Sciences, 2017It has been known from the medical records of Kancheepuram district hospitals that there is a prevalence of Methicillin resistant Staphylococcus aureus strain. Now the recent epidemiology has shown the presence of Methicillin Resistant Staphylococcus aureus even in school going children community individuals. In view of the above situation, Methicillin
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The Molecular Detection of mecA Genes of Staphylococcus Aureus
Annals of Advanced Biomedical Sciences, 2019Antibiotic resistance is common among pathogenic bacteria associated with community acquired and nosocomial infections. Methicillin-resistant Staphylococcus aureus (MRSA) infections have become a global health problem particularly in hospital setup causing simple skin infections to life threatening infections. The present study aimed to investigate the
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Rapid identification of bacteria, mecA and van genes from blood cultures
Pathologie Biologie, 2007The Genotype technology, a quick molecular genetic assay based on DNA multiplex amplification with biotinylated primers followed by hybridization to membrane bound probes, complies with the requirements for a fast diagnosis of sepsis. We evaluated the new Genotype BC Gram-negative and Gram-positive test kits (Hain Life Science, Germany) which ...
M-F, Prère, O, Baron, O, Fayet
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