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Surveillance of variant mecA gene and analysis of efflux pump in mecA negative MRSA infections.

Pakistan journal of pharmaceutical sciences, 2019
It has been known from the medical records of Kancheepuram district hospitals that there is a prevalence of Methicillin resistant Staphylococcus aureus strain. Now the recent epidemiology has shown the presence of Methicillin Resistant Staphylococcus aureus even in school going children community individuals. In view of the above situation, Methicillin
Karthikeyan, Vijayan   +3 more
openaire   +1 more source

The Characterization of mecA Gene and SCCmec Typing in Clinical Samples of MRSA

Science Letters, 2019
MRSA is a major pathogen worldwide and its infections are associated with increased morbidity and mortality, in comparison with other S. aureus infections. The study was designed to characterize the mecA gene and staphylococcal cassette chromosome (SCCmec) in methicillin resistance S. aureus (MRSA). A total of 20 presumptive S.
Aziyah Abdul Aziz, Ali Jawad
openaire   +1 more source

[MRSA--detection of mecA and its regulatory genes].

Rinsho byori. The Japanese journal of clinical pathology, 1994
Methicillin resistance in S.aureus and S.epidermidis strains is primarily due to production of a new penicillin-binding protein PBP2' with extremely low binding affinity to most beta-lactam antibiotics. The structural gene for PBP2', mecA, is detectable in clinical specimens by using the polymerase chain reaction (PCR).
S, Kagawa, K, Yamashita, A, Matsuoka
openaire   +1 more source

[Combination PCR of mecA, femA genes for detection of MRSA].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2004
To make a comparison of PCR assay, Agar Dilution and Disk Diffusion as for detecting Staphylococcal mecA, femA genes.A total of 178 strains of Staphylococci were isolated from three large scale hospitals in Chengdu. Disk Diffusion and staphylococcal mecA, femA gene PCR assay for detecting MRSA were compared with Agar Dilution.The sensitivity and ...
Zhihong, Chen, Xinjian, Fan, Xiaoju, Lü
openaire   +1 more source

An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus

Biosensors and Bioelectronics, 2018
Electrochemical DNA biosensor has unique advantages for on-site pathogenic microorganism detection, yet the detection of long DNA towards genome DNA (gDNA) analysis remains challenge. In this work, we report a novel electrochemical biosensor for the ultrasensitive analysis of mecA DNA on methicillin-resistant Staphylococcus aureus (MRSA) genome, using ...
Li, Xu   +8 more
openaire   +2 more sources

Rapid Detection of the Methicillin-resistance Gene,mecA, in Coagulase-negative Staphylococci

Scandinavian Journal of Infectious Diseases, 2001
Phenotypical methods are routinely used to detect methicillin resistance in Staphylococci. These methods are time-consuming and there are difficulties in detecting all resistant strains carrying the mecA gene. We detected methicillin-resistant Staphylococci in biological samples by PCR amplification of mecA, without the time-consuming step of ...
T, Glad   +4 more
openaire   +2 more sources

Staphylococcus pseudintermedius heterogeneously expresses the mecA gene

Veterinary Microbiology, 2013
Vincenzo, Savini   +5 more
openaire   +2 more sources

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