Results 1 to 10 of about 2,179,747 (304)

PCR biases distort bacterial and archaeal community structure in pyrosequencing datasets [PDF]

open access: yes, 2012
As 16S rRNA gene targeted massively parallel sequencing has become a common tool for microbial diversity investigations, numerous advances have been made to minimize the influence of sequencing and chimeric PCR artifacts through rigorous quality control ...
Ameet J. Pinto   +2 more
core   +23 more sources

Geolocation Inference of Forensic Individual Origin by Soil Metagenomic Analysis [PDF]

open access: yesFayixue Zazhi, 2021
Objective To preliminarily discuss the feasibility of geolocation inference of forensic individual origin by soil metagenomic analysis. Methods The 33 soil samples from Heilongjiang, Qinghai and Tibet were collected, total bacterial DNA in the samples ...
LIU Wen-li, CHENG Feng, QIAN Jia-lin, et al.
doaj   +1 more source

Selective Detection of Nucleotides in Infant Formula Using an N-Rich Covalent Triazine Porous Polymer

open access: yesNanomaterials, 2022
The aromatic structure and the rich nitrogen content of polymers based on covalent triazine-based frameworks (CTF) and their unique hydrophilic-lipophilic-balanced adsorption properties make them promising candidates for an adsorbent that can be used for
Yafei Hou   +8 more
doaj   +1 more source

Advancements in Single-cell Sequencing and the Prospect of Its Forensic Application [PDF]

open access: yesFayixue Zazhi, 2019
Single-cell sequencing is a technique that analyzes DNA and RNA sequences on the cellular level with next generation sequencing. The ultra high resolution of single-cell sequencing provides new perspectives and opens new frontiers for our understanding ...
CHEN Man, YANG Ya-ran, WU Hui-juan, et al.
doaj   +1 more source

Serological and molecular biological analysis of RhD--: a case report

open access: yesZhongguo shuxue zazhi, 2023
Objective To study the blood group serology and molecular biology of patients with RhD--, so as to guide clinical blood use. Methods The EDTA-K2 anticoagulant blood of the patient was detected for Rh antigens and antibodies. Meanwhile, DNA was extracted,
Bo SHUI   +4 more
doaj   +1 more source

Sequencing BGI: the evolution of expertise and research organisation in the world’s leading gene sequencing facility

open access: yesNew Genetics and Society, 2021
The increasing importance of computational techniques in post-genomic life science research calls for new forms and combinations of expertise that cut across established disciplinary boundaries between computing and biology.
Kai Wang, Xiaobai Shen, Robin Williams
doaj   +1 more source

The pros and comms of gene sequencing [PDF]

open access: yes, 2017
Full-gene sequencing undoubtedly comes with its pluses and its minuses. In this article, the authors aim to weigh up the pros and cons not only from the point of view of the patient but also in view of the doctor's possible perspective.
Codacci Pisanelli, Giovanni   +3 more
core   +2 more sources

On the utility of RNA sample pooling to optimize cost and statistical power in RNA sequencing experiments [PDF]

open access: yes, 2020
Background: In gene expression studies, RNA sample pooling is sometimes considered because of budget constraints or lack of sufficient input material. Using microarray technology, RNA sample pooling strategies have been reported to optimize both the cost
Assefa, Alemu Takele   +2 more
core   +2 more sources

A High-Throughput Method for Illumina RNA-Seq Library Preparation. [PDF]

open access: yes, 2012
With the introduction of cost effective, rapid, and superior quality next generation sequencing techniques, gene expression analysis has become viable for labs conducting small projects as well as large-scale gene expression analysis experiments. However,
Daniel H Chitwood   +8 more
core   +2 more sources

Aeromonas veronii Causes Hemorrhagic Septicemia in Cultured Nile tilapia in Qena Governorate

open access: yesSVU-International Journal of Veterinary Sciences, 2022
n summer 2021, acute mortalities occurred in Nile tilapia farm in Qena governorate. Different hypothesis including the implication of environmental factors, and/or pathogens have been explored.
Karima A. Bakry, Walaa F.A. Emeish
doaj   +1 more source

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