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Mammalian Gene Transfer and Gene Expression
1986It has long been the desire of molecular biologists to be able to study the regulation of expression of specific mammalian genes. With the advent of recombinant DNA technology and the availability of specific cloned genes, several new approaches to these studies have emerged.
T E, Wagner, X Z, Chen, W B, Hayes
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Detecting Laterally Transferred Genes
2012Methods for identifying alien genes in genomes fall into two general classes. Phylogenetic methods examine the distribution of a gene's homologues among genomes to find those with relationships not consistent with vertical inheritance. These approaches include identifying orphan genes which lack homologues in closely related genomes and genes with ...
Rajeev K, Azad, Jeffrey G, Lawrence
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Bulletin de l'Academie nationale de medecine, 2001
Mammalian cells in culture can be modified by gene transfer and these procedures are routinely used in experimental biology. Yet, efficient approaches to modify certain complex cell populations, stem cells or cells organized within a tissue are still lacking.
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Mammalian cells in culture can be modified by gene transfer and these procedures are routinely used in experimental biology. Yet, efficient approaches to modify certain complex cell populations, stem cells or cells organized within a tissue are still lacking.
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2009
Horizontal gene and genome transfer forces us to recognize that life evolves by fusion as well as bifurcation of lineages, and necessitates the expansion of traditional views of evolution. This chapter reviews the role that horizontal gene transfer (HGT) may play in integrating selection at the gene, species, and community levels.
Lorraine, Olendzenski +1 more
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Horizontal gene and genome transfer forces us to recognize that life evolves by fusion as well as bifurcation of lineages, and necessitates the expansion of traditional views of evolution. This chapter reviews the role that horizontal gene transfer (HGT) may play in integrating selection at the gene, species, and community levels.
Lorraine, Olendzenski +1 more
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Gene Transfer: Transformation/Electroporation
2014Since Pseudomonas aeruginosa is a non-naturally competent bacterium, various methods have been developed to transfer exogenous DNA. Alternatively to transduction and conjugation, electroporation can also be used to transfer exogenous DNA molecules into Pseudomonas.
Cadoret, Frederic +2 more
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Lateral gene transfer and the nature of bacterial innovation
Nature, 2000H. Ochman, J. Lawrence, E. Groisman
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Ancient horizontal gene transfer
Nature Reviews Genetics, 2003The cornerstone of Charles Darwin's theory of evolution is the vertical inheritance of traits from parent to offspring across successive generations. However, molecular evolutionary biologists have shown that extensive horizontal (also known as lateral) gene transfer (HGT) can occur between distantly related species.
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1987
Publisher Summary Transgenic mice provide a powerful assay system to characterize temporal and spatial patterns of gene expression to analyze the role of specific molecules in complex biological phenomena and alter the physiology of whole animals in defined ways. This chapter reviews the broad areas in which transgenic mice have made or are likely to
G, Scangos, C, Bieberich
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Publisher Summary Transgenic mice provide a powerful assay system to characterize temporal and spatial patterns of gene expression to analyze the role of specific molecules in complex biological phenomena and alter the physiology of whole animals in defined ways. This chapter reviews the broad areas in which transgenic mice have made or are likely to
G, Scangos, C, Bieberich
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Direct gene transfer into mouse muscle in vivo.
Science, 1990J. Wolff +6 more
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