Results 341 to 350 of about 10,802,441 (400)
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Current Protocols in Human Genetics, 2001
AbstractThis unit provides a set of protocols for introducing recombinant genes into normal, injured, and atherosclerotic arteries. The protocols include animal preparation, surgical techniques, and delivery systems. Protocols describe gene delivery to normal, injured, and stented porcine iliofemoral arteries, employing a double balloon infusion ...
Ripudamanjit Singh +4 more
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AbstractThis unit provides a set of protocols for introducing recombinant genes into normal, injured, and atherosclerotic arteries. The protocols include animal preparation, surgical techniques, and delivery systems. Protocols describe gene delivery to normal, injured, and stented porcine iliofemoral arteries, employing a double balloon infusion ...
Ripudamanjit Singh +4 more
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Tissue Engineering, 1995
The cardiovascular system is a promising site for gene delivery because of its large surface area and direct contact with the blood stream. Gene transfer into vascular cells by adenoviral and retroviral vectors or liposomes has been demonstrated in several cell types and animal models.
Amy D. Ouellette +2 more
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The cardiovascular system is a promising site for gene delivery because of its large surface area and direct contact with the blood stream. Gene transfer into vascular cells by adenoviral and retroviral vectors or liposomes has been demonstrated in several cell types and animal models.
Amy D. Ouellette +2 more
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Science, 1987
Until recently, gene transfer in plants was achieved only by sexual hybridization. Now, in addition, plant genetic manipulation, with the use of both recombinant DNA and protoplast fusion technology, is being applied to an increasing range of plants. The soil bacterium Agrobacterium tumefaciens , with its associated
Michael R. Davey, Edward C. Cocking
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Until recently, gene transfer in plants was achieved only by sexual hybridization. Now, in addition, plant genetic manipulation, with the use of both recombinant DNA and protoplast fusion technology, is being applied to an increasing range of plants. The soil bacterium Agrobacterium tumefaciens , with its associated
Michael R. Davey, Edward C. Cocking
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Gene Transfer to the Cerebellum
The Cerebellum, 2010There are several diseases for which gene transfer therapy to the cerebellum might be practicable. In these studies, we used recombinant Tag-deleted SV40-derived vectors (rSV40s) to study gene delivery targeting the cerebellum. These vectors transduce neurons and microglia very effectively in vitro and in vivo, and so we tested them to evaluate gene ...
Elisabeth J. Van Bockstaele +3 more
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Blood, 1999
C LINICAL GENE THERAPY is going through an uncomfortable adolescence. Many observers have already been disappointed by the lack of clear-cut successes with this strategy. To this sense of frustration has now been added a feeling of dismay because of the death of an 18-year-old with ...
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C LINICAL GENE THERAPY is going through an uncomfortable adolescence. Many observers have already been disappointed by the lack of clear-cut successes with this strategy. To this sense of frustration has now been added a feeling of dismay because of the death of an 18-year-old with ...
openaire +3 more sources
1987
Publisher Summary Transgenic mice provide a powerful assay system to characterize temporal and spatial patterns of gene expression to analyze the role of specific molecules in complex biological phenomena and alter the physiology of whole animals in defined ways. This chapter reviews the broad areas in which transgenic mice have made or are likely to
George A. Scangos, Charles J. Bieberich
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Publisher Summary Transgenic mice provide a powerful assay system to characterize temporal and spatial patterns of gene expression to analyze the role of specific molecules in complex biological phenomena and alter the physiology of whole animals in defined ways. This chapter reviews the broad areas in which transgenic mice have made or are likely to
George A. Scangos, Charles J. Bieberich
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Direct gene transfer to protoplasts: Fate of the transferred genes
Developmental Genetics, 1990AbstractDirect gene transfer to protoplasts is one of several methods developed for the production of transgenic plants. This method utilizes the efficient uptake of DNA from the surrounding medium by protoplasts (cell wall‐less plant cells). Where a suitable protoplast system exists large numbers of transformant clones can be efficiently produced and ...
Ingo Potrykus, Michael W. Saul
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Current Opinion in Lipidology, 1997
Vascular gene transfer may be useful for the treatment of several cardiovascular diseases. It can also be used as an experimental tool to test the effects of various genes in a local vascular compartment. Promising therapeutic effects have been obtained in animal models of restenosis with the transfer of vascular endothelial growth factor, nitric oxide
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Vascular gene transfer may be useful for the treatment of several cardiovascular diseases. It can also be used as an experimental tool to test the effects of various genes in a local vascular compartment. Promising therapeutic effects have been obtained in animal models of restenosis with the transfer of vascular endothelial growth factor, nitric oxide
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Gene transfer by electroporation
Molecular Biotechnology, 1997Electroporation of cells in the presence of DNA is widely used for the introduction of transgenes either stably or transiently into bacterial, fungal, animal, and plant cells. A review of the literature shows that electroporation parameters are often reported in an incomplete or incorrect manner, forcing researchers to rely too much on a purely ...
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Horizontal gene transfer and adaptive evolution in bacteria
Nature Reviews Microbiology, 2021Brian J. Arnold, I-Ting Huang, W. Hanage
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