Results 101 to 110 of about 582,689 (334)
Fast targeted gene transfection and optogenetic modification of single neurons using femtosecond laser irradiation [PDF]
, 2013 This work is supported by the UK Engineering Physical Sciences Research Council (EPSRC).A prevailing problem in neuroscience is the fast and targeted delivery of DNA into selected neurons.
Antkowiak, Maciej +5 more
core +1 more source
Molecular Oncology, EarlyView.Combining melting curve analysis enhances the multiplexing capability of digital PCR. Here, we developed a 14‐plex assay to simultaneously measure single nucleotide mutations and amplifications of KRAS and GNAS, which are common driver genes in pancreatic cancer precursors. This assay accurately quantified variant allele frequencies in clinical samples
Junko Tanaka +10 more
wiley +1 more source
Progress in the use of adeno-associated viral vectors for gene therapy [PDF]
, 2004 The development of safe and efficient gene transfer vectors is crucial for the success of gene therapy trials. A viral vector system promising to meet these requirements is based on the apathogenic adeno-associated virus (AAV-2), a member of the ...
Braun-Falco, M., Buning, H., Hallek, M.
core +1 more source
Molecular Oncology, EarlyView.TGF‐β has a complex role in cancer, exhibiting both tumor‐suppressive and tumor‐promoting properties. Using a series of differentiated tumoroids, derived from different stages and mutational background of colorectal cancer patients, we replicate this duality of TGF‐β in vitro. Notably, the atypical but highly aggressive KRASQ22K mutation rendered early‐
Theresia Mair +17 more
wiley +1 more source
Recent Progresses in Brain Gene Therapy
International Clinical Neuroscience Journal, 2016 Gene therapy consists of the introduction of genetic material into cells for a therapeutic purpose. The understanding of the basics of the molecular and cellular mechanisms in disease treatments has resulted in the development of high-tech gene transfer ...
Afsoun Seddighi +5 more
doaj +1 more source
Concise Review: The Potential Use of Intestinal Stem Cells to Treat Patients With Intestinal Failure. [PDF]
, 2016 : Intestinal failure is a rare life-threatening condition that results in the inability to maintain normal growth and hydration status by enteral nutrition alone.
Dunn, James CY +3 more
core +1 more source
Molecular Oncology, EarlyView.Chronic TGF‐β exposure drives epithelial HCC cells from a senescent state to a TGF‐β resistant mesenchymal phenotype. This transition is characterized by the loss of Smad3‐mediated signaling, escape from senescence, enhanced invasiveness and metastatic potential, and upregulation of key resistance modulators such as MARK1 and GRM8, ultimately promoting
Minenur Kalyoncu +11 more
wiley +1 more source
Mechanisms and kinetic assays of aminoacyl‐tRNA synthetases
FEBS Letters, EarlyView.
Igor Zivkovic +2 more
wiley +1 more source
Molecular Oncology, EarlyView.Dual targeting of AKT and mTOR using MK2206 and RAD001 reduces tumor burden in an intracardiac colon cancer circulating tumor cell xenotransplantation model. Analysis of AKT isoform‐specific knockdowns in CTC‐MCC‐41 reveals differentially regulated proteins and phospho‐proteins by liquid chromatography coupled mass spectrometry. Circulating tumor cells
Daniel J. Smit +19 more
wiley +1 more source
Assessing Plant Genetic Diversity by Molecular Tools
Diversity, 2009 This paper is an overview of the diverse, predominantly molecular techniques, used in assessing plant genetic diversity. In recent years, there has been a significant increase in the application of molecular genetic methods for assessing the conservation
Linda Mondini +2 more
doaj +1 more source