Results 291 to 300 of about 1,319,898 (363)
Techniques for gene transfer into neurons
Current Opinion in Neurobiology, 2002 To illuminate the function of the thousands of genes that make up the complexity of the nervous system, it is critical to be able to introduce and express DNA in neurons. Over the past two decades, many gene transfer methods have been developed, including viral vectors, liposomes and electroporation.
Philip Washbourne +1 more
semanticscholar +5 more sources
Gene-transfer and plant-regeneration (techniques)
Trends in Biotechnology, 1995 The production of transgenic plants depends on the stable introduction of foreign DNA into the plant genome, followed by regeneration to produce intact plants, and the subsequent expression of the introduced gene(s). A variety of transformation methods have been developed, and this has allowed many of the world's important crop plants to be transformed.
Richard Walden, Ruth Wingender
semanticscholar +4 more sources
Gene Transfer Techniques to Study Neuropeptide Processing
Annual Review of Physiology, 1988 Giju Thomas +2 more
semanticscholar +5 more sources
Some of the next articles are maybe not open access.
Related searches:
Related searches:
Gene Transfer Techniques and their relevance to Woody Plants
2000For decades, the concept of foreign DNA transfer into plant cells and its integration into the genome of the recipient cell was an intriguing curiosity (Lurquin, 1977). It was not until the advent of Southern hybridization (Southern, 1975) and the availability of restriction enzymes that the presence of `non-plant’ DNA (in this case, the T-DNA of ...
Minocha, Subhash C., Wallace, John C.
openaire +4 more sources
Gene transfer to plants: approaches and available techniques [PDF]
, 1993 Gene transfer to plants is hampered by a variety of biological problems: the plant cell wall is a perfect barrier and trap for DNA molecules; egg cells, sperm cells and zygotes, are virtually inaccessible; proembryos are extremely small and enclosed within solid tissues; the tiny cells of hidden meristems which contribute to the ‘germline’ may not be ...
I. Potrykus
openaire +2 more sources
Techniques of Chromosome-Mediated Gene Transfer
, 1982 Functional genes can be transferred to eukaryotic cells by incubation with isolated metaphase chromosomes. Uptake occurs by phagocytosis and most of the ingested chromosomal DNA is degraded to small inactive fragments. The frequency of transfer of any selected marker ranges from about 10-5 to 10-7.
O. Wesley McBride
openalex +3 more sources
Comparison of different techniques for gene transfer into mature and immature tobacco pollen
Transgenic Research, 1992The particle gun, cocultivation withAgrobacterium tumefaciens, and imbibition in DNA solutions were compared as methods to transfer DNA into mature and immature pollen ofNicotiana tabacum. Bombardment of mature pollen with the β-glucuronidase gene cloned behind the pollen-specific PA2 promoter of the chalcone isomerase gene ofPetunia hybrida resulted ...
Stöger, Eva +4 more
openaire +4 more sources
Gene transfer techniques for CNS organotypic cultures
Neuroscience Research, 2009Takayasu Mikuni +2 more
openaire +3 more sources
Gene transfer and antisense nucleic acid techniques
Parasitology Today, 1994Attempts to suppress a harmful genetic trait by antisense means, or to restore a normal phenotype by gene transfer, attract much publicity. This is especially the case where clinical trials incorporating such methodologies have been initiated, such as antisense oligonucleotide therapies for some types of leukaemia, antisense gene-transfer therapy for a
R.G. Vile, N. Miller
openaire +3 more sources