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Physical methods of gene transfer: Kinetics of gene delivery into cells: A Review

, 2015
The ability to introduce isolated DNA into cells has tremendous influence on advances of molecular biology. Recently, with the development of attractive strategies for gene therapy, successful gene delivery has gained importance once again and become a ...
A. Das, Parul Gupta, D. Chakraborty
semanticscholar   +1 more source

Techniques of DNA-Mediated Gene Transfer for Eukaryotic Cells

1982
DNA transformation in bacteria has permitted detailed genetic analysis as well as the implementation of recombinant DNA technology. The utility of manipulation and cloning of genes in bacteria is well recognized. This chapter will concern the manipulation and cloning of genes in eukaryotic systems ranging from yeast to animal systems. Gene manipulation
Arthur P. Bollon, Saul J. Silverstein
openaire   +2 more sources

Growth Factors and Gene Transfer with DNA Strand Technique in Tendon Healing

Journal of Long-Term Effects of Medical Implants, 2002
Growth factors are likely to be critically involved in all aspects of tendon healing and many studies, in vitro and in vivo, have tried to define the role of growth factors in this process, and to determine appropriate strategies for their use in tissue engineering to improve the healing of tendons.
William C. Lineaweaver, Feng Zhang
openaire   +3 more sources

Magnetic nanoparticle-mediated gene transfer to oligodendrocyte precursor cell transplant populations is enhanced by magnetofection strategies.

ACS Nano, 2011
This study has tested the feasibility of using physical delivery methods, employing static and oscillating field "magnetofection" techniques, to enhance magnetic nanoparticle-mediated gene transfer to rat oligodendrocyte precursor cells derived for ...
Stuart I Jenkins   +3 more
semanticscholar   +1 more source

Lipospermine-mediated gene transfer technique into murine cultured cortical cells

Journal of Neuroscience Methods, 1997
In order to transfer exogenous DNA into embryonic cortical cells, we have chosen a transfection technique using a synthetic lipospermine (dipalmitoylphosphatidylethanolamylspermine, DPPES) which complexes DNA molecules and allows their penetration into the intracellular compartment.
Sola, Brigitte   +4 more
openaire   +4 more sources

p53 Plasmid Preparation and Techniques for Analysis of Gene Transfer and Expression

2003
Hepatocellular carcinoma (HCC) is one of the most common causes of cancer death worldwide and is especially prevalent in certain areas of Africa and Asia. The most important etiological factor is infection with the hepatitis B or C virus. Treatment is generally unsatisfactory as the majority of patients are not suitable for surgical resection and ...
Satoko Negishi   +5 more
openaire   +3 more sources

Transfer Learning for Molecular Cancer Classification Using Deep Neural Networks

IEEE/ACM Transactions on Computational Biology & Bioinformatics, 2019
The emergence of deep learning has impacted numerous machine learning based applications and research. The reason for its success lies in two main advantages: 1) it provides the ability to learn very complex non-linear relationships between features and ...
R. K. Sevakula   +4 more
semanticscholar   +1 more source

Plant Transformation Techniques: Agrobacterium- and Microparticle-Mediated Gene Transfer in Cereal Plants

2020
Biotechnological methods for targeted gene transfers into plants are key for successful breeding in the twenty-first century and thus essential for the survival of humanity. Two decades ago, genetic transformation of crop plants was not routine, and it was all but impossible with important cereals such as barley and wheat.
Karl-Heinz Kogel, Jafargholi Imani
openaire   +3 more sources

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