Evolution of Bacterial Gene Transfer Agents [PDF]
AbstractBacterial gene transfer agents (GTAs) are small virus-like particles that package DNA fragments and inject them into cells. They are encoded by gene clusters resembling defective prophages, with genes for capsid head and tail components. These gene clusters are usually assumed to be maintained by selection for the benefits of GTA-mediated ...
Redfield, Rosemary J., Soucy, Shannon M.
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The emergence and fate of horizontally acquired genes in Escherichia coli [PDF]
Bacterial species, and even strains within species, can vary greatly in their gene contents and metabolic capabilities. We examine the evolution of this diversity by assessing the distribution and ancestry of each gene in 13 sequenced isolates of ...
Marri, P.R.+2 more
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Genome-wide association analysis identifies resistance loci for bacterial blight in a diverse collection of indica rice germplasm [PDF]
Bacterial blight, which is caused by Xanthomonas oryzae pv. oryzae (Xoo), is one of the most devastating rice diseases worldwide. The development and use of disease-resistant cultivars have been the most effective strategy to control bacterial blight ...
Fan Zhang+7 more
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Bacterial Genes Outnumber Archaeal Genes in Eukaryotic Genomes [PDF]
Abstract Eukaryotes are typically depicted as descendants of archaea, but their genomes are evolutionary chimeras with genes stemming from archaea and bacteria. Which prokaryotic heritage predominates? Here, we have clustered 19,050,992 protein sequences from 5,443 bacteria and 212 archaea with 3,420,731 protein sequences from 150 ...
Brückner, Julia, Martin, William F.
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Reservoir of Bacterial Exotoxin Genes in the Environment [PDF]
Many bacteria produce secreted virulence factors called exotoxins. Exotoxins are often encoded by mobile genetic elements, including bacteriophage (phage). Phage can transfer genetic information to the bacteria they infect. When a phage transfers virulence genes to an avirulent bacterium, the bacterium can acquire the ability to cause disease.
Scott T. Kelley+4 more
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Functional Transcriptomics for Bacterial Gene Detectives [PDF]
ABSTRACT Developments in transcriptomic technology and the availability of whole-genome-level expression profiles for many bacterial model organisms have accelerated the assignment of gene function. However, the deluge of transcriptomic data is making the analysis of gene expression a challenging task for biologists.
Jay C. D. Hinton+1 more
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Increased variability of microbial communities in restored salt marshes nearly 30 years after tidal flow restoration [PDF]
We analyzed microbial diversity and community composition from four salt marsh sites that were impounded for 40–50 years and subsequently restored and four unimpounded sites in southeastern Connecticut over one growing season.
Bernhard, Anne E+2 more
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cDNA-RNA subtractive hybridization reveals increased expression of mycocerosic acid synthase in intracellular Mycobacterium bovis BCG. [PDF]
Identifying genes that are differentially expressed by Mycobacterium bovis BCG after phagocytosis by macrophages will facilitate the understanding of the molecular mechanisms of host cell-intracellular pathogen interactions. To identify such genes a cDNA-
Centre Gunnels Wood+7 more
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Bacterial CRISPR screens for gene function
In this review we describe the application of CRISPR tools for functional genomics screens in bacteria, with a focus on the use of interference (CRISPRi) approaches. We review recent developments in CRISPRi titration, which has enabled essential gene functional screens, and genome-scale pooled CRISPRi screens.
Todor, Horia+3 more
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Functional Traits Co-Occurring with Mobile Genetic Elements in the Microbiome of the Atacama Desert [PDF]
Mobile genetic elements (MGEs) play an essential role in bacterial adaptation and evolution. These elements are enriched within bacterial communities from extreme environments. However, very little is known if specific genes co-occur with MGEs in extreme
Airo, Alessandro+4 more
core +1 more source