Results 201 to 210 of about 6,955,736 (304)

Nanotechnology Strategies in Plant Genetic Engineering: Intelligent Delivery and Precision Editing. [PDF]

Plants (Basel)
Lai CM, Xiao XS, Liu LW, Lin XD, Dou DL, Cai HY, Mei ZF, Yang F, Cheng Y, Qin Y.   +9 more
europepmc   +1 more source

Engineering IL‐4 resistant proinflammatory human myeloid cells for cancer immunotherapy

Molecular Oncology, EarlyView.
We developed a scalable workflow to generate proinflammatory human myeloid cells. CRISPR/Cas9‐edited CD34+ hematopoietic stem and progenitor cells were expanded and differentiated with M‐CSF. Deletion of STAT6 or STAT6/NFKB1 enhanced macrophage proinflammatory gene expression and cytokine secretion in the presence of IL‐4 while maintaining antibody ...
Theresa Barberi, Alan D. Friedman
wiley   +1 more source

Genomics, genetic engineering and artificial cells

Annals of Indian Academy of Neurology, 2010
Thomas Sanjeev
doaj  

Re-Tooling of γδ T Cells for Cancer Immunotherapy Using Advanced Manufacturing and Genetic Engineering. [PDF]

Cells
Lim BJL, Maher J.
europepmc   +1 more source

Spatial heterogeneity of hotspot mutations and clonal relatedness in ovarian endometrioid carcinoma

Molecular Oncology, EarlyView.
Spatially resolved analysis revealed heterogeneous distributions of selected cancer‐associated mutations within ovarian endometrioid carcinomas. Shared mutations between paired endometrial and ovarian tumors supported clonal relatedness. These findings highlight the value of sampling multiple tumor regions when interpreting molecular profiles ...
Takahito Ashihara, Kosuke Murakami, Shiro Takamatsu, Akiko Kanto, Seiichi Mori, Tomoyuki Otani, Kazuko Sakai, Yasushi Kotani, Kazuto Nishio, Kaoru Abiko, Noriomi Matsumura   +10 more
wiley   +1 more source

Optimizing genetic engineering approaches for protein loading into bacterial extracellular vesicles for vaginal drug delivery. [PDF]

J Control Release
Steinman D, Hemanth Kumar VS, McIlvaine RA, Tyagi P, Kang H, Alam R, Liu A, Jewell CM, Plotkin A, Burd I, Molinari S, Zierden HC.   +11 more
europepmc   +1 more source

Raman‐based label‐free microscopic analysis of the pancreas in living zebrafish larvae

FEBS Open Bio, EarlyView.
Forward stimulated Raman scattering (F‐SRS) and epi coherent anti‐Stokes Raman scattering (E‐CARS) allow label‐free discrimination of distinct subcellular structures in the pancreas of living zebrafish larvae. Given the straightforward applicability, we anticipate broad implementation of Raman microscopy in other organs and across various biomedical ...
Noura Faraj, Eline M. F. de Lange, Klaas A. Sjollema, Ben N. G. Giepmans   +3 more
wiley   +1 more source

Genetic Engineering of Lysogenic-Lytic Switch Genes Improves <i>Burkholderia</i> Phage Killing Efficacy. [PDF]

Int J Mol Sci
Khrongsee P, Doore SM, Somprasong N, Schweizer HP, Xiao YP, Subramaniam K, Mergia A, Tuanyok A.   +7 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Existentialism and My 'Postwolf' Dachshund: Authenticity in the Age of Genetic Engineering. [PDF]

Bioethics
Thaldar D.
europepmc   +1 more source