Results 11 to 20 of about 733,956 (256)
Agronomy, 2021 After a basic description of the different sets of genetic tools and genomic approaches most relevant for modern crop breeding (e. g., QTL mapping, GWAS and genomic selection; transcriptomics, qPCR and RNA-seq; transgenesis and gene editing), this review
Elena Benavente, Estela Giménez
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Transgene-free genome editing and RNAi ectopic application in fruit trees: Potential and limitations
Frontiers in Plant Science, 2022 For the past fifteen years, significant research advances in sequencing technology have led to a substantial increase in fruit tree genomic resources and databases with a massive number of OMICS datasets (transcriptomic, proteomics, metabolomics ...
Satyanarayana Gouthu +4 more
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Precision genetics tools for genetic improvement of banana
The Plant Genome, 2023 Abstract Banana is an important food security crop for millions of people in the tropics but it faces challenges from diseases and pests. Traditional breeding methods have limitations, prompting the exploration of precision genetic tools like genetic modification and genome editing.
Jaindra Nath Tripathi +2 more
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Microorganisms, 2022 The secretion of recombinant proteins plays an important role in their economic production and purification. The secretion efficiency depends on the responsible signal peptide (SP) in combination with the target protein and the given host and cannot be ...
Janine Mayer +6 more
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A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila
eLife, 2020 Genetic screens are powerful tools for the functional annotation of genomes. In the context of multicellular organisms, interrogation of gene function is greatly facilitated by methods that allow spatial and temporal control of gene abrogation.
Fillip Port +18 more
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Frontiers in Bioengineering and Biotechnology, 2022 The yeast Ogataea polymorpha is an upcoming host for bio-manufacturing due to its unique physiological properties, including its broad substrate spectrum, and particularly its ability to utilize methanol as the sole carbon and energy source.
Katrin Wefelmeier +3 more
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Advancing Genetic Tools in Streptococcus pneumoniae. [PDF]
Genes (Basel), 2020 Streptococcus pneumoniae is the causative agent of a multitude of diseases, and further study into its pathogenies is vital. The pneumococcus is genetically malleable, and several tools are available to manipulate this pathogen. In this study, we attempted to utilize one such tool, the Sweet Janus cassette, to replace the capsule locus with other ...
Echlin H, Rosch JW.
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Overview of the genetic tools in the Archaea [PDF]
Frontiers in Microbiology, 2012 This section provides an overview of the genetic systems developed in the Archaea. Genetic manipulation is possible in many members of the halophiles, methanogens, Sulfolobus, and Thermococcales. We describe the selection/counterselection principles utilized in each of these groups, which consist of antibiotics and their resistance markers, and ...
Haruyuki Atomi +2 more
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Development of Genetic Modification Tools for Hanseniasporauvarum [PDF]
International Journal of Molecular Sciences, 2021 Apiculate yeasts belonging to the genus Hanseniaspora are commonly isolated from viticultural settings and often dominate the initial stages of grape must fermentations. Although considered spoilage yeasts, they are now increasingly becoming the focus of research, with several whole-genome sequencing studies published in recent years.
Jennifer Badura +6 more
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Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons
eLife, 2022 Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells.
Lewis Macdonald +8 more
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