Results 21 to 30 of about 752,866 (297)

Mix and Match: Promoters and Terminators for Tuning Gene Expression in the Methylotrophic Yeast Ogataea polymorpha

Frontiers in Bioengineering and Biotechnology, 2022
The yeast Ogataea polymorpha is an upcoming host for bio-manufacturing due to its unique physiological properties, including its broad substrate spectrum, and particularly its ability to utilize methanol as the sole carbon and energy source.
Katrin Wefelmeier, Birgitta E. Ebert, Lars M. Blank, Simone Schmitz   +3 more
doaj   +1 more source

Overview of the genetic tools in the Archaea [PDF]

Frontiers in Microbiology, 2012
This section provides an overview of the genetic systems developed in the Archaea. Genetic manipulation is possible in many members of the halophiles, methanogens, Sulfolobus, and Thermococcales. We describe the selection/counterselection principles utilized in each of these groups, which consist of antibiotics and their resistance markers, and ...
Haruyuki Atomi, Tadayuki Imanaka, Toshiaki Fukui   +2 more
openaire   +3 more sources

Development of Genetic Modification Tools for Hanseniasporauvarum [PDF]

International Journal of Molecular Sciences, 2021
Apiculate yeasts belonging to the genus Hanseniaspora are commonly isolated from viticultural settings and often dominate the initial stages of grape must fermentations. Although considered spoilage yeasts, they are now increasingly becoming the focus of research, with several whole-genome sequencing studies published in recent years.
Jennifer Badura, Niël van Wyk, Silvia Brezina, Isak S. Pretorius, Doris Rauhut, Jürgen Wendland, Christian von Wallbrunn   +6 more
openaire   +2 more sources

Rapid and specific degradation of endogenous proteins in mouse models using auxin-inducible degrons

eLife, 2022
Auxin-inducible degrons are a chemical genetic tool for targeted protein degradation and are widely used to study protein function in cultured mammalian cells.
Lewis Macdonald, Gillian C Taylor, Jennifer Margaret Brisbane, Ersi Christodoulou, Lucy Scott, Alex von Kriegsheim, Janet Rossant, Bin Gu, Andrew J Wood   +8 more
doaj   +1 more source

Application of the endogenous CRISPR-Cas type I-D system for genetic engineering in the thermoacidophilic archaeon Sulfolobus acidocaldarius

Frontiers in Microbiology, 2023
CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems are widely distributed among bacteria and archaea. In this study, we demonstrate the successful utilization of the type I-D CRISPR-Cas system for genetic engineering in the ...
Jan Bost, Alejandra Recalde, Bianca Waßmer, Alexander Wagner, Bettina Siebers, Sonja-Verena Albers   +5 more
doaj   +1 more source

The GenoChip: A New Tool for Genetic Anthropology [PDF]

Genome Biology and Evolution, 2013
The Genographic Project is an international effort aimed at charting human migratory history. The project is nonprofit and nonmedical, and, through its Legacy Fund, supports locally led efforts to preserve indigenous and traditional cultures. Although the first phase of the project was focused on uniparentally inherited markers on the Y-chromosome and ...
Elhaik E, Greenspan E, Staats S, Krahn T, Tyler-Smith C, Xue Y, Tofanelli S, Francalacci P, Cucca F, Pagani Luca, Jin L, Li H, Schurr TG, Greenspan B, Spencer Wells R, Acosta O, Adhikarla S, Adler CJ, Balanovska E, Balanovsky O, Bertranpetit J, Clarke AC, Comas D, Cooper A, Dulik MC, Gaieski JB, Ganesh Prasad AK, Haak W, Haber M, Kaplan ME, Lacerda DR, Li S, Martinez-Cruz B, Matisoo-Smith EA, Merchant NC, Mitchell JR, Owings AC, Parida L, Pitchappan R, Platt DE, Quintana-Murci L, Renfrew C, Royyuru AK, Sandoval JR, Santhakumari AV, Santos FR, Der Sarkissian CSI, Soodyall S, Soria Hernanz DF, Swamikrishnan P, Vieira PP, Vilar MG, Zalloua PA, Ziegle JS   +53 more
openaire   +4 more sources

Identification of XylR, the Activator of Arabinose/Xylose Inducible Regulon in Sulfolobus acidocaldarius and Its Application for Homologous Protein Expression

Frontiers in Microbiology, 2020
The thermophilic archaeon Sulfolobus acidocaldarius can use different carbon sources for growth, including the pentoses D-xylose and L-arabinose. In this study, we identified the activator XylR (saci_2116) responsible for the transcriptional regulation ...
Nienke van der Kolk, Alexander Wagner, Alexander Wagner, Michaela Wagner, Michaela Wagner, Bianca Waßmer, Bettina Siebers, Sonja-Verena Albers   +7 more
doaj   +1 more source

Modern Tools for Genetic Engineering [PDF]

, 2016
Site-specific endonucleases create double-strand breaks within the genome and can be targeted to literally any genetic mutation. Together with a repair template, a correction of the defective locus becomes possible.

core   +1 more source

Different Tools on Multi-Objective Optimization of a Hybrid Artificial Neural Network – Genetic Algorithm for Plasma Chemical Reactor Modelling [PDF]

, 2012
A hybrid ANN-GA was successfully developed to model, to simulate and to optimize simultaneously a catalytic–DBD plasma reactor. The integrated ANN-GA method facilitates powerful modeling and multi-objective optimization for co-generation of synthesis ...
Istadi, Istadi, I. Istadi, Saidina Amin, Nor Aishah, Nor Aishah Saidina Amin   +3 more
core   +1 more source

Tools for the genetic manipulation of Herpetomonas muscarum [PDF]

G3 Genes|Genomes|Genetics, 2020
Abstract Trypanosomatid parasites are causative agents of important human and animal diseases such as sleeping sickness and leishmaniasis. Most trypanosomatids are transmitted to their mammalian hosts by insects, often belonging to Diptera (or true flies).
Sloan, MA, Ligoxygakis, P
openaire   +4 more sources