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Genetic Transformation in Citrus [PDF]
The Scientific World Journal, 2013 Citrus is one of the world’s important fruit crops. Recently, citrus molecular genetics and biotechnology work have been accelerated in the world. Genetic transformation, a biotechnological tool, allows the release of improved cultivars with desirable characteristics in a shorter period of time and therefore may be useful in citrus breeding programs ...
Donmez D. +4 more
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Genetic Transformation of Yeast [PDF]
BioTechniques, 2001 Genetic transformation was first described by Griffith in 1928 and has since been demonstrated in a variety of organisms, including many species of fungi. This review focuses on the history and technology of the transformation of Saccharomyces cerevisiae. The application of protocols developed for S.
R. D. Gietz, Robin A. Woods
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Genetic Transformation in Yeasts [PDF]
Journal of General Microbiology, 1974 SUMMARY: The transformability of 25 strains of yeasts belonging to 23 species in three genera - Saccharomyces, Hansenula and Candida - was investigated with respect to ten characters. Eighteen hour cultures at about the end of the log phase of growth, addition of 10 μg DNA/ml, and an incubation time of 30 min at 30 to 37 °C usually yielded best results.
N. C. Khan, S. P. Sen
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Genetic transformation of the dinoflagellate chloroplast [PDF]
eLife, 2018 Coral reefs are some of the most important and ecologically diverse marine environments. At the base of the reef ecosystem are dinoflagellate algae, which live symbiotically within coral cells. Efforts to understand the relationship between alga and coral have been greatly hampered by the lack of an appropriate dinoflagellate genetic transformation ...
Adrian C. Barbrook +11 more
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Genetic Transformation and Complementation [PDF]
Methods in molecular biology, 2017 The disciplines of Borrelia (Borreliella) burgdorferi microbiology and Lyme disease pathogenesis have come to depend on the genetic manipulation of the spirochete. Generating mutants in these recalcitrant bacteria, while not straightforward, is routinely accomplished in numerous laboratories, although there are several crucial caveats to consider. This
D. Scott Samuels +2 more
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Genetic Transformation in Rhizobium [PDF]
Journal of General Microbiology, 1969 SUMMARY: Mutants of ground nut (Rhizobium cowpea group) strain gr3 were isolated using ultraviolet radiation and N-methyl-N'-nitrosoguanidine (NTG) as mutagenic agents. An adenine-requiring mutant was used as the recipient and its wild type as the donor in transformation studies. Initially minimal medium supplemented with 2 μg. adenine/ml. was used for
V. V. Modi, J. L. Raina
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Genetic Transformation in Pseudomonas [PDF]
Journal of General Microbiology, 1967 SUMMARY: Intraspecific and interspecific transformation in Pseudomonas has been investigated. Two isoleucine+valine requiring mutants of Pseudomonas aeruginosa have been transformed to prototrophy. By using gelatin liqui-faction as a marker character it was observed that two strains of P. fluorescens, a phytopathogen P.
N. C. Khan, S. P. Sen
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Genetic transformation of Veillonella parvula [PDF]
FEMS Microbiology Letters, 2011 Veillonellae are one of the most prevalent and predominant microorganisms in both the supra- and subgingival plaques of the human oral cavity. Veillonellae's mutualistic relationships with the early, middle, and late colonizers of the oral cavity make them an important component of oral biofilm ecology.
Justin Merritt, Fengxia Qi, Jinman Liu
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Genetic transformation of eucalyptus [PDF]
Physiology and Molecular Biology of Plants, 2011 Eucalyptus is the second most widely planted multipurpose woody tree species in the world. It is a commercially important hardwood tree for paper and wood industries. In the past two decades, various research groups reported different genetic transformation protocols and attempts towards development of transgenic eucalyptus. Much of the work related to
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Genetic Transformation in Conifers [PDF]
, 1999 Several attempts at the genetic improvement of tree species have been made, but in comparison with crop plants the efforts as well as the results have been rather limited. The most commonly used approaches have involved selection of superior genotypes from natural outbred populations, mutations, and intra- and inter-specific hybridization under ...
Minocha, Subhash C., Minocha, Rakesh
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