Results 231 to 240 of about 546,143 (293)

Mapping Hsp104 interactions using cross‐linking mass spectrometry

FEBS Open Bio, EarlyView.
This study examines how cross‐linking mass spectrometry can be utilized to analyze ATP‐induced conformational changes in Hsp104 and its interactions with substrates. We developed an analytical pipeline to distinguish between intra‐ and inter‐subunit contacts within the hexameric homo‐oligomer and discovered contacts between Hsp104 and a selected ...
Kinga Westphal, Karolina Michalska, Andrzej Joachimiak, Lukasz A. Joachimiak   +3 more
wiley   +1 more source

Establishment and application of highly efficient regeneration, genetic transformation and genome editing system for cucurbitacins biosynthesis in Hemsleya chinensis. [PDF]

BMC Plant Biol
Wang J, Li CH, Xiang CF, Zhou PH, Li LS, Li X, Yang SC, Zhang GH, Zhao Y.   +8 more
europepmc   +1 more source

Dysfunctional tetraspanin 7 (TSP‐7) in Caenorhabditis elegans promotes; increases in average life‐ & health‐span, stress‐induced survival and motility

FEBS Open Bio, EarlyView.
The C. elegans tetraspanin‐7 (tsp‐7) is a homologue of human CD63, which is a negative regulator of autophagy. The C. elegans strain, tm5761, has a dysfunctional (knockout) tsp‐7 gene. When compared to the wild‐type strain, the tm5761 strain shows increased: life‐ and health‐span; thermotolerance, and stress‐induced locomotion.
Brogan Jones, Laurence Seabra, Francesco Michelangeli   +2 more
wiley   +1 more source

Genetic transformation in conifers: current status and future prospects. [PDF]

For Res (Fayettev)
Zhao H, Zhang J, Zhao J, Niu S.
europepmc   +1 more source

Genetic transformation in a synchronous culture of Streptococcus pneumoniae.

, 1989
Takeshi Fujii, Akihiko Kudou, Hiroyuki Seto   +2 more
openalex   +2 more sources

A non‐fluorescent immunohistochemistry method for measuring autophagy flux using MAP1LC3/LC3 and SQSTM1 as core markers

FEBS Open Bio, EarlyView.
We introduce an immunohistochemistry method to measure autophagy flux, highlighting the active degradation and recycling of cellular waste. This cost‐effective approach uses tissue samples to track key markers like LC3 and SQSTM1, revealing how cells maintain health or respond to diseases such as cancer. It bridges the gap between research and clinical
Shahla Shojaei, Amir Barzegar Behrooz, Marco Cordani, Mahmoud Aghaei, Negar Azarpira, Daniel J. Klionsky, Saeid Ghavami   +6 more
wiley   +1 more source

A Fast, Efficient, and Tissue-Culture-Independent Genetic Transformation Method for Panax notoginseng and Lilium regale. [PDF]

Plants (Basel)
Deng J, Li W, Li X, Liu D, Liu G.
europepmc   +1 more source

Genetic transformation of brassica napus via agrobacterium spp. and the development of herbicide resistance.

, 1989
Pierre Charest
openalex   +2 more sources

Enhancing transporter activity in heterologous expression systems with SAHA: a 2500‐times more potent and odorless alternative to butyrate

FEBS Open Bio, EarlyView.
Heterologous expression of membrane transporters in cultured cells is essential for functional characterization, but is sometimes limited by low activity. Our study compares the HDAC inhibitors butyrate, VPA and SAHA to enhance transport activity. We propose to replace butyrate by SAHA: it is equally effective, devoid of repulsive odor, costs less, and
Svenja Flögel, Maurice Tust, Samira Boussettaoui, Dietmar Fischer, Dirk Gründemann   +4 more
wiley   +1 more source

Establishment of an Efficient Genetic Transformation System in Sanghuangporus baumii. [PDF]

J Fungi (Basel)
Wang X, Wang M, Sun J, Qu X, Wang S, Sun T.   +5 more
europepmc   +1 more source