Results 251 to 260 of about 546,143 (293)

Construction and optimization of a genetic transformation system for efficient expression of human insulin-GFP fusion gene in flax. [PDF]

Bioresour Bioprocess
Zhao W, Zhang R, Zhou L, Zhang Z, Du F, Wu R, Kong J, An S.   +7 more
europepmc   +1 more source

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, EarlyView.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

Genetic transformation of the frog-killing chytrid fungus Batrachochytrium dendrobatidis. [PDF]

Proc Natl Acad Sci U S A
Kalinka E, Brody SM, Swafford AJM, Medina EM, Fritz-Laylin LK.   +4 more
europepmc   +1 more source

Genetic transformation in Streptococcus pneumoniae: nucleotide sequence and predicted amino acid sequence of recP

, 1990
B A Radnis, Dong‐Kwon Rhee, Donald A. Morrison   +2 more
openalex   +1 more source

Enhanced discovery of bacterial laccase‐like multicopper oxidase through computer simulation and metagenomic analysis of industrial wastewater

FEBS Open Bio, EarlyView.
We obtained potential bacterial laccase‐like multicopper oxidase (LMCO) sequences through metagenomic sequencing. All sequences exhibited significant differences from known LMCOs in databases. To select the most promising candidates, we performed structure prediction and molecular docking using alphafold2, metal3d and rosetta.
Ting Cui, Ariel Kushmaro, Hana Barak, Anja Poehlein, Rolf Daniel, Hans‐Jürgen Mägert   +5 more
wiley   +1 more source

Production of hairy root cultures of lettuce (Lactuca sativa L.)

Open Life Sciences, 2014
Vojin Tadić, Snežana Milošević, Aleksandar Cingel, Marija Petrić, Milana Trifunović, Dragana Antonić, Jovan Tadić, Angelina Subotić   +7 more
doaj   +1 more source

Simple and Fail-safe Method to Transform Miniprep Escherichia coli Strain K12 Plasmid DNA Into Viable Agrobacterium tumefaciens EHA105 Cells for Plant Genetic Transformation. [PDF]

Bio Protoc
Siamalube B, Ehinmitan E, Ngotho M, Onguso J, Runo S.   +4 more
europepmc   +1 more source

Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins

FEBS Open Bio, EarlyView.
With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet, Julia Bartoli, Atakan Kabtan, Chloé M. Piras, Audrey C. Tempier, Eric Cascales, Julie P. Viala   +6 more
wiley   +1 more source

KCS1 and VIP1, the genes encoding yeast phosphoinositol pyrophosphate synthases, are required for Ca2+‐mediated response to dimethylsulfoxide (DMSO)

FEBS Open Bio, EarlyView.
Ca2+‐mediated response to DMSO was investigated in Saccharomyces cerevisiae cells expressing Ca2+‐dependent aequorin. Cell exposure to DMSO induced a cytosolic Ca2+ wave dependent on the integrity of the Cch1/Mid1 channel. Deletion of KCS1 or VIP1 genes encoding the phosphoinositol pyrophosphate (PP‐IP) synthases suppressed the DMSO‐induced Ca2 ...
Larisa Ioana Gogianu, Lavinia Liliana Ruta, Claudia Valentina Popa, Simona Ghenea, Ileana Cornelia Farcasanu   +4 more
wiley   +1 more source

A method of genetic transformation and gene editing of succulents without tissue culture. [PDF]

Plant Biotechnol J
Lu J, Li S, Deng S, Wang M, Wu Y, Li M, Dong J, Lu S, Su C, Li G, Lang Z, Zhu JK.   +11 more
europepmc   +1 more source