Results 91 to 100 of about 4,360,463 (355)

Properties and use of novel replication-competent vectors based on Semliki Forest virus

Virology Journal, 2009
Background Semliki Forest virus (SFV) has a positive strand RNA genome and infects different cells of vertebrates and invertebrates. The 5' two-thirds of the genome encodes non-structural proteins that are required for virus replication and synthesis of ...
Lulla Valeria, Karo-Astover Liis, Ülper Liane, Iofik Anna, Rausalu Kai, Merits Andres   +5 more
doaj   +1 more source

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

Geminiviruses and their application in biotechnology

Journal of Integrative Agriculture, 2017
Being a major class of single-stranded DNA viruses, geminiviruses are mostly studied due to their catastrophic infectious effect on crops. These DNA viruses are characteristic for their ability in quickly multiplying viral genetic materials without ...
Qiu-ying YANG, Bo DING, Xue-ping ZHOU
doaj  

A toolkit for Nannochloropsis oceanica CCMP1779 enables gene stacking and genetic engineering of the eicosapentaenoic acid pathway for enhanced long‐chain polyunsaturated fatty acid production

Plant Biotechnology Journal, 2017
Summary Nannochloropsis oceanica is an oleaginous microalga rich in ω3 long‐chain polyunsaturated fatty acids (LC‐PUFAs) content, in the form of eicosapentaenoic acid (EPA). We identified the enzymes involved in LC‐PUFA biosynthesis in N.
Eric Poliner, Jane A. Pulman, K. Zienkiewicz, K. Childs, C. Benning, E. M. Farré   +5 more
semanticscholar   +1 more source

Loss of proton‐sensing GPR4 reduces tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
G protein‐coupled receptor 4 (GPR4) is a pH‐sensing receptor activated by acidic pH. GPR4 expression is increased in patients with inflammatory bowel disease who are at high risk of developing colorectal cancer. In mouse models, loss of GPR4 attenuated tumor progression. This correlated with increased IL2 and natural killer cell activity.
Leonie Perren, Moana Busch, Pedro A. Ruiz, Ermanno Malagola, Valeria Baumeler, Federica Foti, Adelina Gross, Tobias Grütter, Hendrik Edel, Cordelia Schuler, Kristina Handler, Glenn De Lange, Isabelle C. Arnold, Cheryl de Vallière, Klaus Seuwen, Martin Hausmann, Gerhard Rogler   +16 more
wiley   +1 more source

Practical challenges in data‐driven interpolation: Dealing with noise, enforcing stability, and computing realizations

International Journal of Adaptive Control and Signal Processing, EarlyView., 2023
Summary In this contribution, we propose a detailed study of interpolation‐based data‐driven methods that are of relevance in the model reduction and also in the systems and control communities. The data are given by samples of the transfer function of the underlying (unknown) model, that is, we analyze frequency‐response data.
Quirin Aumann, Ion Victor Gosea
wiley   +1 more source

Applications and developments of gene therapy drug delivery systems for genetic diseases

Asian Journal of Pharmaceutical Sciences, 2021
Genetic diseases seriously threaten human health and have always been one of the refractory conditions facing humanity. Currently, gene therapy drugs such as siRNA, shRNA, antisense oligonucleotide, CRISPR/Cas9 system, plasmid DNA and miRNA have shown ...
Xiuhua Pan, Hanitrarimalala Veroniaina, Nan Su, Kang Sha, Fenglin Jiang, Zhenghong Wu, Xiaole Qi   +6 more
doaj  

The art of vector engineering: towards the construction of next‐generation genetic tools

Microbial Biotechnology, 2018
When recombinant DNA technology was developed more than 40 years ago, no one could have imagined the impact it would have on both society and the scientific community. In the field of genetic engineering, the most important tool developed was the plasmid
L. C. Nora, C. Westmann, Leonardo Martins-Santana, L. Alves, L. Monteiro, M. Guazzaroni, Rafael Silva-Rocha   +6 more
semanticscholar   +1 more source

TRPM8 levels determine tumor vulnerability to channel agonists

Molecular Oncology, EarlyView.
TRPM8 is a Ca2+ permissive channel. Regardless of the amount of its transcript, high levels of TRPM8 protein mark different tumors, including prostate, breast, colorectal, and lung carcinomas. Targeting TRPM8 with channel agonists stimulates inward calcium currents followed by emptying of cytosolic Ca2+ stores in cancer cells.
Alessandro Alaimo, Francesco Giuseppe Carbone, Kristi Buzo, Nicole Annesi, Sacha Genovesi, Annalisa Lorenzato, Karen Widmann, Michela Libergoli, Elisa Marmocchi, Giovanni Bertalot, Alberto Brolese, Mauro Giulio Papotti, Luca Molinaro, Orazio Caffo, Mattia Barbareschi, Alberto Bardelli, Alessandro Romanel, Sabrina Arena, Andrea Lunardi   +18 more
wiley   +1 more source

Data‐driven performance metrics for neural network learning

International Journal of Adaptive Control and Signal Processing, EarlyView., 2023
Summary Effectiveness of data‐driven neural learning in terms of both local mimima trapping and convergence rate is addressed. Such issues are investigated in a case study involving the training of one‐hidden‐layer feedforward neural networks with the extended Kalman filter, which reduces the search for the optimal network parameters to a state ...
Angelo Alessandri, Mauro Gaggero, Marcello Sanguineti   +2 more
wiley   +1 more source