Results 71 to 80 of about 629,088 (301)

A framework for quantification and physical modeling of cell mixing applied to oscillator synchronization in vertebrate somitogenesis [PDF]

, 2017
In development and disease, cells move as they exchange signals. One example is found in vertebrate development, during which the timing of segment formation is set by a ‘segmentation clock’, in which oscillating gene expression is synchronized across a ...
Bhavna, Rajasekaran, Morelli, Luis Guillermo, Oates, Andrew C., Uriu, Koichiro   +3 more
core   +4 more sources

The planar cell polarity protein Vangl2 interacts with the PDZ‐domains of Scribble but not with a unique PDZ‐like domain in Inturned

FEBS Letters, EarlyView.
Structural and biochemical characterisations show that the planar cell polarity (PCP) protein Inturned harbours a unique PDZ‐like domain that does not bind canonical PDZ‐binding motifs (PBMs) like that of another PCP protein Vangl2. In contrast, the apical‐basal polarity protein Scribble contains four PDZ domains that bind Vangl2, but one PDZ domain ...
Stephan Wilmes, Jan Brysch, Carmen Gelze, Lilli Meier, Daniel Kümmel   +4 more
wiley   +1 more source

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Developing Universal Genetic Tools for Rapid and Efficient Deletion Mutation in Vibrio Species Based on Suicide T-Vectors Carrying a Novel Counterselectable Marker, vmi480. [PDF]

PLoS ONE, 2015
Despite that Vibrio spp. have a significant impact on the health of humans and aquatic animals, the molecular basis of their pathogenesis is little known, mainly due to the limited genetic tools for the functional research of genes in Vibrio.
Peng Luo, Xiangyan He, Qiuting Liu, Chaoqun Hu   +3 more
doaj   +1 more source

Applications and developments of gene therapy drug delivery systems for genetic diseases

Asian Journal of Pharmaceutical Sciences, 2021
Genetic diseases seriously threaten human health and have always been one of the refractory conditions facing humanity. Currently, gene therapy drugs such as siRNA, shRNA, antisense oligonucleotide, CRISPR/Cas9 system, plasmid DNA and miRNA have shown ...
Xiuhua Pan, Hanitrarimalala Veroniaina, Nan Su, Kang Sha, Fenglin Jiang, Zhenghong Wu, Xiaole Qi   +6 more
doaj   +1 more source

Particle Swarm Optimization Framework for Low Power Testing of VLSI Circuits

, 2011
Power dissipation in sequential circuits is due to increased toggling count of Circuit under Test, which depends upon test vectors applied. If successive test vectors sequences have more toggling nature then it is sure that toggling rate of flip flops is
Khosla, Arun, Narang, Sukhleen Bindra, Singh, Balwnder   +2 more
core   +2 more sources

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Phosphorylation-Dependent Assembly of a 14-3-3 Mediated Signaling Complex during Red Blood Cell Invasion by Plasmodium falciparum Merozoites

mBio, 2020
Red blood cell (RBC) invasion by Plasmodium merozoites requires multiple steps that are regulated by signaling pathways. Exposure of P. falciparum merozoites to the physiological signal of low K+, as found in blood plasma, leads to a rise in cytosolic ...
Kunal R. More, Inderjeet Kaur, Quentin Giai Gianetto, Brandon M. Invergo, Thibault Chaze, Ravi Jain, Christéle Huon, Petra Gutenbrunner, Hendrik Weisser, Mariette Matondo, Jyoti S. Choudhary, Gordon Langsley, Shailja Singh, Chetan E. Chitnis   +13 more
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

NDRG2 Drives DRP1 Phosphorylation to Promote the Progression of Pulmonary Hypertension

Frontiers in Bioscience-Landmark
Background: Pulmonary hypertension (PH) is a progressive disease characterized by obstructive pulmonary vascular remodeling, for which no curative therapies effectively reverse disease progression. This study investigated whether N-myc
Jiayong Luo, Jinyu Chang, Yi Liu, Yusang Shao, Yanyan Zhang, Yingru Zheng, Enfang Xiao, Yanqing Xie, Shuangshuang Tu, Haoxuan Lu, Wenming He   +10 more
doaj   +1 more source