Results 51 to 60 of about 2,681,594 (304)

Structural and biochemical analysis of a B12 superbinder

FEBS Letters, EarlyView.
BtuG proteins are vitamin B12 scavengers in Bacteroides thetaiotaomicron, a dominant human gut bacterium. We present crystal structures of three BtuG homologs bound to cobalamin and its precursor cobinamide, revealing picomolar binding affinities, among the highest known for any natural protein.
Jose M. Martinez Felices, Jacob J. Whittaker, Dirk J. Slotboom, Albert Guskov   +3 more
wiley   +1 more source

Aspongopus chinensis-derived cytochrome c delivered via lentivirus-like particles induces mitochondrial apoptosis to inhibit HER2-positive breast cancer

陆军军医大学学报
Objective Human epidermal growth factor receptor 2 (HER2)-positive breast cancer accounts for approximately 20% of all breast cancer cases. Persistent activation of the HER2/PI3K/AKT signaling pathway enables tumor cells to evade mitochondrial apoptosis
Li Xiping, Li Xiping, Wang Min, Peng Li   +3 more
doaj   +1 more source

Malaria Control with Genetically Manipulated Insect Vectors

Science, 2002
At a recent workshop, experts discussed the benefits, risks, and research priorities associated with using genetically manipulated insects in the control of vector-borne diseases.
Alphey L., Beard C., Billingsley P., Coetzee M., CRISANTI, Andrea, Curtis C., Eggleston P., Godfray C., Hemingway J., Jacobs Lorena M., James A., Kafatos F., Mukwaya L., Paton M., Powell J., Schneider W., Scott T., Sina B., Sinkins S., Spielman A., Toure Y., Collins F., Sinden R.   +22 more
openaire   +4 more sources

Structures of mycobacterial 3‐methylcrotonyl‐CoA carboxylase reveal carrier‐domain translocation between catalytic sites

FEBS Letters, EarlyView.
Mycobacterial 3‐methylcrotonyl‐CoA carboxylase uses a mobile biotin‐carrying domain to shuttle a carboxyl group between two catalytic sites, enabling carboxylation of 3‐methylcrotonyl‐CoA during leucine breakdown. Cryo‐electron microscopy captures the carrier at both sites and reveals an inward loop movement that may prevent futile rebinding to the ...
Ajit Yadav, Bogdan I. Florea, Sebastian Geibel   +2 more
wiley   +1 more source

Viral Hybrid Vectors for Somatic Integration - Are They the Better Solution? [PDF]

, 2009
The turbulent history of clinical trials in viral gene therapy has taught us important lessons about vector design and safety issues. Much effort was spent on analyzing genotoxicity after somatic integration of therapeutic DNA into the host genome. Based
Anja Ehrhardt, Nadine Müther, Ehrhardt, Anja, Nadja Noske, Müther, Nadine, Noske, Nadja, Nadine Müther   +6 more
core   +1 more source

Developing Universal Genetic Tools for Rapid and Efficient Deletion Mutation in Vibrio Species Based on Suicide T-Vectors Carrying a Novel Counterselectable Marker, vmi480. [PDF]

PLoS ONE, 2015
Despite that Vibrio spp. have a significant impact on the health of humans and aquatic animals, the molecular basis of their pathogenesis is little known, mainly due to the limited genetic tools for the functional research of genes in Vibrio.
Peng Luo, Xiangyan He, Qiuting Liu, Chaoqun Hu   +3 more
doaj   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

FEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge, Vivian Pogenberg, Alexander Baumgart, Bente Siebels, Hartmut Schlüter, Michael Hecht‐Bucher, Aymelt Itzen   +6 more
wiley   +1 more source

Genetic Algorithm Assisted Minimum Bit Error Rate Multiuser Detection in Multiple Antenna Aided OFDM

, 2004
The family of minimum bit error rate (MBER) multiuser detectors (MUD) is capable of outperforming the classic minimum mean-squared-error (MMSE)MUDin term of the achievable bit-error rate (BER) owing to directly minimising the BER cost function.
Alias, M.Y., S. Chen, Chen, Sheng, Alias, M. Y., L. Hanzo, M.Y. Alias, Hanzo, L., Chen, S.   +7 more
core   +2 more sources

Applications and developments of gene therapy drug delivery systems for genetic diseases

Asian Journal of Pharmaceutical Sciences, 2021
Genetic diseases seriously threaten human health and have always been one of the refractory conditions facing humanity. Currently, gene therapy drugs such as siRNA, shRNA, antisense oligonucleotide, CRISPR/Cas9 system, plasmid DNA and miRNA have shown ...
Xiuhua Pan, Hanitrarimalala Veroniaina, Nan Su, Kang Sha, Fenglin Jiang, Zhenghong Wu, Xiaole Qi   +6 more
doaj   +1 more source

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

FEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil, Jeffrey A. Hawkes, Cecilia Blikstad, Pia Lindberg   +3 more
wiley   +1 more source