Rescue of splicing-mediated intron loss maximizes expression in lentiviral vectors containing the human ubiquitin C promoter. [PDF]
, 2014 Lentiviral vectors almost universally use heterologous internal promoters to express transgenes. One of the most commonly used promoter fragments is a 1.2-kb sequence from the human ubiquitin C (UBC) gene, encompassing the promoter, some enhancers, first
Cooper, Aaron R +3 more
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Population-based incremental learning with memory scheme for changing environments [PDF]
, 2005 Copyright @ 2005 ACMIn recent years there has been a growing interest in studying evolutionary algorithms for dynamic optimization problems due to its importance in real world applications.
Yang, S
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BiomedicinesBackground: The biosafety of gene therapy products remains a major challenge to their introduction into the clinic. In particular, the problem of immunogenicity of viral vectors is the focus of attention.
Andrei Izmailov +11 more
doaj +1 more source
Leishmania donovani populations in Eastern Sudan: temporal structuring and a link between human and canine transmission. [PDF]
, 2014 BACKGROUND: Visceral leishmaniasis (VL), caused by the members of the Leishmania donovani complex, has been responsible for devastating VL epidemics in the Sudan.
Baleela, Rania +6 more
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Genetic modification of baculovirus expression vectors
Virologica Sinica, 2012 As a protein expression vector, the baculovirus demonstrates many advantages over other vectors. With the development of biotechnology, baculoviral vectors have been genetically modified to facilitate high level expression of heterologous proteins in both insect and mammalian cells.
Shu-fen, Li +3 more
openaire +3 more sources
Pleiotropy of FRIGIDA enhances the potential for multivariate adaptation. [PDF]
, 2013 An evolutionary response to selection requires genetic variation; however, even if it exists, then the genetic details of the variation can constrain adaptation.
Easlon, Hsien M +9 more
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The genetic diversity and geographical separation study of Oncomelania hupensis populations in mainland China using microsatellite loci [PDF]
, 2016 © 2016 Guan et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
Abe, EM +5 more
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CRISPR/Cas9‐mediated genome editing: from basic research to translational medicine [PDF]
, 2020 The recent development of the CRISPR/Cas9 system as an efficient and accessible programmable genome-editing tool has revolutionized basic science research. CRISPR/Cas9 system-based technologies have armed researchers with new powerful tools to unveil the
Ferreira, B I +2 more
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Use of herpes virus amplicon vectors to study brain disorders
BioTechniques, 2005 There is an enormous initiative to establish the genetic basis for disorders of brain function. Unfortunately, genetic intervention is not accomplished easily in the nervous system.
Rachael L. Neve +3 more
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Colony polymerase chain reaction of stably transfected Trypanosoma cruzi grown on solid medium [PDF]
Memorias do Instituto Oswaldo Cruz, 2000 Tools for the genetic manipulation of Trypanosoma cruzi are largely unavailable, although several vectors for transfection of epimastigotes and expression of foreign or recombinant genes have been developed. We have previously constructed several plasmid
Wagner G dos Santos +2 more
doaj +1 more source