Results 71 to 80 of about 4,784,986 (369)
Vacuolar transport and function of Saccharomyces cerevisiae sterol ester hydrolase Tgl1
FEBS Letters, EarlyView.Tgl1, one of yeast sterol ester hydrolases, had been found on the lipid droplets where sterol esters are mainly stored. This study revealed that Tgl1 is transported into the vacuole depending on the ESCRT‐I–III complex, and that it exhibits intra‐vacuolar sterol ester hydrolase activity.
Takumi Nakatsuji +5 more
wiley +1 more source
Frontiers in Neuroscience, 2023 IntroductionIntracranial hemorrhage detection in 3D Computed Tomography (CT) brain images has gained more attention in the research community. The major issue to deal with the 3D CT brain images is scarce and hard to obtain the labelled data with better ...
Jewel Sengupta +3 more
doaj +1 more source
Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium [PDF]
, 2015 © 2015 Webster et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
A. A. Hamidou +23 more
core +4 more sources
New and Redesigned pRS Plasmid Shuttle Vectors for Genetic Manipulation of Saccharomyces cerevisiae
G3: Genes, Genomes, Genetics, 2012 We have constructed a set of 42 plasmid shuttle vectors based on the widely used pRS series for use in the budding yeast Saccharomyces cerevisiae and the bacterium Escherichia coli. This set of pRSII plasmids includes new shuttle vectors that can be used
M. Chee, S. Haase
semanticscholar +1 more source
FEBS Letters, EarlyView.In this study, we present the structure of AcrIE8.1, a previously uncharacterized anti‐CRISPR protein that inhibits the type I‐E CRISPR‐Cas system. Through a combination of structural and biochemical analyses, we demonstrate that AcrIE8.1 directly binds to the Cas11 subunit of the Cascade complex to inhibit the CRISPR‐Cas system.
Young Woo Kang, Hyun Ho Park
wiley +1 more source
A test problem for visual investigation of high-dimensional multi-objective search [PDF]
, 2014 An inherent problem in multiobjective optimization is that the visual observation of solution vectors with four or more objectives is infeasible, which brings major difficulties for algorithmic design, examination, and development.
Miqing Li +5 more
core +4 more sources
A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins
FEBS Letters, EarlyView.We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue +6 more
wiley +1 more source
Structural analysis of biodiversity. [PDF]
PLoS ONE, 2010 Large, recently-available genomic databases cover a wide range of life forms, suggesting opportunity for insights into genetic structure of biodiversity.
Lawrence Sirovich +2 more
doaj +1 more source
Expression systems for industrial Gram-positive bacteria with low guanine and cytosine content [PDF]
, 1997 Recent years have seen an increase in the development of gene expression systems for industrial Gram-positive bacteria with low guanine and cytosine content that belong to the genera Bacillus, Clostridium, Lactococcus, Lactobacillus, Staphylococcus and ...
Kleerebezem, Michiel, +2 more
core +2 more sources
mBio, 2019 We have entered an era when studies of the gut microbiota are transitioning from basic questions of composition and host effects to understanding the microbial molecules that underlie compositional shifts and mediate health and disease processes.
Leonor García-Bayona, L. Comstock
semanticscholar +1 more source