Results 11 to 20 of about 439,037 (382)

New multiplex PCR methods for rapid screening of genetically modified organisms in foods. [PDF]

Front Microbiol, 2015
We present novel multiplex PCR methods for rapid and reliable screening of genetically modified organisms (GMOs). New designed PCR primers targeting four frequently used GMO specific sequences permitted identification of new DNA markers, in particular ...
Datukishvili N, Kutateladze T, Gabriadze I, Bitskinashvili K, Vishnepolsky B.   +4 more
europepmc   +2 more sources

Genetically Modified Organisms

The American Biology Teacher, 2018
Food Fight: GMO's and the future of the American Diet . By McKay Jenkins. 2017. Avery. (ISBN: 9781594634604). 322 Pages. Hardcover $26.00. McKay Jenkins has traveled across the country interviewing people about where our food comes from and the impact modern agricultural practices have on the quality of the food we eat as well as the impact these ...
Jonathan Morris
openaire   +2 more sources

Food safety, food security and genetically modified organisms in Africa: a current perspective

Biotechnology & Genetic Engineering Reviews, 2021
Moving forward from 2020, Africa faces an eminent challenge of food safety and security in the coming years. The World Food Programme (WFP) of the United Nations (UN) estimates that 20% of Africa’s population of 1.2 billion people face the highest level ...
Sefater Gbashi, O. Adebo, J. A. Adebiyi, S. Targuma, S. Tebele, O. M. Areo, B. Olopade, J. O. Odukoya, P. Njobeh   +8 more
semanticscholar   +1 more source

Effect of the Matrix and Target on the Accurate Quantification of Genomic and Plasmid DNA by Digital Polymerase Chain Reaction

Agriculture, 2023
In polymerase chain reaction (PCR)-based nucleic acid quantification, the DNA template type, primer/probe sequence, and instrument platform such as real-time quantitative PCR (qPCR) and digital PCR (dPCR) affect the accuracy and reliability of ...
Nengwu Si, Jun Li, Hongfei Gao, Yunjing Li, Shanshan Zhai, Fang Xiao, Li Zhang, Gang Wu, Yuhua Wu   +8 more
doaj   +1 more source

A Label-Free Electrochemical Impedance Genosensor Coupled with Recombinase Polymerase Amplification for Genetically Modified Maize Detection

Agriculture, 2022
As the cultivation scale of genetically modified (GM) crops strongly increases, a convenient DNA assay is highly demanded in resource-limited areas. A label-free electrochemical impedance (EI) genosensor using gold carbon dots (GCDs) was developed with ...
Dandan Cui, Shanshan Zhai, Yao Yang, Yuhua Wu, Jun Li, Xiaohong Yan, Ping Shen, Hongfei Gao, Gang Wu   +8 more
doaj   +1 more source

Optimization of digital droplet polymerase chain reaction for quantification of genetically modified organisms. [PDF]

Biomol Detect Quantif, 2016
Gerdes L, Iwobi A, Busch U, Pecoraro S.
europepmc   +2 more sources

Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example

Food Chemistry: Molecular Sciences, 2022
Basic data for the safety assessment of transgenic line involves the molecular characterization of the integration site of exogenous DNA, flanking sequences, copy number, and unintended plasmid backbone residues.
Hanwen Zhang, Yuchen Zhang, Wenting Xu, Rong Li, Dabing Zhang, Litao Yang   +5 more
doaj   +1 more source

Development of a qualitative real-time PCR method to detect 19 targets for identification of genetically modified organisms. [PDF]

Springerplus, 2016
Peng C, Wang P, Xu X, Wang X, Wei W, Chen X, Xu J.   +6 more
europepmc   +3 more sources

Metabolic engineering of Escherichia coli for 2,4-dinitrotoluene degradation

Ecotoxicology and Environmental Safety, 2023
2,4-Dinitrotoluene (2,4-DNT) as a common industrial waste has been massively discharged into the environment with industrial wastewater. Due to its refractory degradation, high toxicity, and bioaccumulation, 2,4-DNT pollution has become increasingly ...
Wen-Hui Zhang, Yong-Dong Deng, Zhi-Feng Chen, Zhi-Hao Zuo, Yong-Sheng Tian, Jing Xu, Bo Wang, Li-Juan Wang, Hong-Juan Han, Zhen-Jun Li, Yu Wang, Quan-Hong Yao, Jian-Jie Gao, Xiao-Yan Fu, Ri-He Peng   +14 more
doaj   +1 more source

Collaborative Ring Trial of the Applicability of a Reference Plasmid DNA Calibrant in the Quantitative Analysis of GM Maize Event MON810

Foods, 2022
Certified reference materials (CRMs) is one of the critical requirements in a quantitative analytical method, such as in the quantification of genetically modified (GM) contents in food/feed products.
Yanan Meng, Shu Wang, Jinchao Guo, Litao Yang   +3 more
doaj   +1 more source