Results 71 to 80 of about 125,784 (265)

CRISPR-FRT targets shared sites in a knock-out collection for off-the-shelf genome editing

Nature Communications, 2018
Genome editing requires precise targeting of loci with specific gRNAs. Here the authors introduce CRISPR-FRT, which targets flippase recognition sites, common in bacterial genetic collections, for fast off-the-shelf genome engineering.
Toon Swings, David C. Marciano, Benu Atri, Rachel E. Bosserman, Chen Wang, Marlies Leysen, Camille Bonte, Thomas Schalck, Ian Furey, Bram Van den Bergh, Natalie Verstraeten, Peter J. Christie, Christophe Herman, Olivier Lichtarge, Jan Michiels   +14 more
doaj   +1 more source

Bacterial‐Electrochemical Platform Utilizing a MXene‐Peptide Hydrogel

Advanced Functional Materials, EarlyView.
A peptide‐based fibrillar hydrogel incorporating MXene facilitates efficient electron delivery to intracellular recombinant [FeFe]‐hydrogenase enzyme in E. coli, enabling sustained bioelectrochemical H2 production without engineered exoelectrogenicity pathways.
Oren Ben‐Zvi, Itzhak Grinberg, Or Messer, Dana Cohen‐Gerassi, Maxim Sokol, Iftach Yacoby, Lihi Adler‐Abramovich   +6 more
wiley   +1 more source

Association of gut microbiota with metabolism in juvenile Atlantic salmon

Microbiome, 2020
The gut microbiome plays a key role in animal health and metabolism through the intricate functional interconnection between the feed, gut microbes, and the host.
H. Dvergedal, S. R. Sandve, I. L. Angell, G. Klemetsdal, K. Rudi   +4 more
doaj   +1 more source

Advances in Sustainable and Wearable Textile Based Soft Robotics

Advanced Functional Materials, EarlyView.
This Review examines advances in wearable textile‐based soft robotics, focusing on sustainable materials, integrated sensing, and scalable actuation. It discusses manufacturing and system integration across healthcare, assistive robotics, prosthetics, and human–machine interfaces, and highlights key challenges in circular design, including life‐cycle ...
Zahir Abbas, Marzia Dulal, Sophia K. Novoselov, Victoria K. Novoselov, Kostya S. Novoselov, Shaila Afroj, Nazmul Karim   +6 more
wiley   +1 more source

Photothermal‐Activated Antibacterial Amyloid‐Polyphenol‐Iron Hydrogels for Synergistic Wound Healing

Advanced Healthcare Materials, EarlyView.
We report a thermally triggered supramolecular hydrogel (LTFe) formed by Fe3+ and tannic acid on lysozyme amyloid fibrils. Fe3+ enables rapid gelation and photothermal transduction with an efficiency of 88.56%. The LTFe hydrogel exhibits excellent biocompatibility, potent antibacterial activity against E. coli and S.
Di Wu, Jiangtao Zhou, Yang Shen, Qiyao Sun, Tong Li, Xiaoyang Zou, Bin Liu, Hongshan Liang, Raffaele Mezzenga   +8 more
wiley   +1 more source

The probiotic Lactiplantibacillus plantarum ATCC10241 inhibits wound-associated pathogens through the secretion of organic acids

Scientific Reports
While probiotics are traditionally studied for their role in gut health, they are also being explored for their potential to treat wound infections and enhance wound healing.
Sybren Van Ginneken, Bram Lories, Hans P. Steenackers   +2 more
doaj   +1 more source

Assembling metagenomes, one community at a time

BMC Genomics, 2017
Background Metagenomics allows unprecedented access to uncultured environmental microorganisms. The analysis of metagenomic sequences facilitates gene prediction and annotation, and enables the assembly of draft genomes, including uncultured members of a
Andries Johannes van der Walt, Marc Warwick van Goethem, Jean-Baptiste Ramond, Thulani Peter Makhalanyane, Oleg Reva, Don Arthur Cowan   +5 more
doaj   +1 more source

Spontaneous Helical Alignment of Smooth Muscle Cells to Form a Medial Layer for Engineered Microvasculature

Advanced Healthcare Materials, EarlyView.
Spontaneous helical alignment of smooth muscle cells is induced within resistance‐vessel‐sized channels patterned within a hydrogel. The extent of the cells’ orientation angle is dependent on the presence and composition of ECM proteins lining the channel wall and cell seeding density.
Victoria D. Vest, Katherine J. Young, Isabella K. Holtz, Mark Mc Veigh, James D. West, Leon M. Bellan   +5 more
wiley   +1 more source

Self‐Assembled Skin Equivalents with Monoclonal CRISPR/Cas9‐Modified N/TERT‐1 Keratinocytes: A Cutting‐Edge model for Human Skin and its Diseases

Advanced Healthcare Materials, EarlyView.
Self‐assembled, scaffold‐free full‐thickness skin equivalents with monoclonal, genetically modified N/TERT‐1 keratinocytes represent a novel in vitro model of human skin and skin diseases. The model is highly robust, reproducible, physiologically relevant, and suitable for high‐throughput applications.
Marta Slaufova, Tugay Karakaya, Michela Di Filippo, Thomas Kündig, Hans‐Dietmar Beer   +4 more
wiley   +1 more source

Natural Biomaterials for Osteochondral Repair: From Source to Strategy

Advanced Healthcare Materials, EarlyView.
Biological origin‐guided overview of natural biomaterials and therapeutic strategies for osteochondral tissue engineering. The circular diagram categorizes representative materials and strategies into plant/algae‐derived, microbial‐derived, animal‐derived, and human‐derived sources, centered on an osteochondral defect repair model.
Hengyu Liu, Hanyang Zhang, Wenbo Yang, Hao Chen, Jincheng Wang, Fei Chang   +5 more
wiley   +1 more source