Results 21 to 30 of about 449,272 (264)

CRISPR/FnCas12a-mediated efficient multiplex and iterative genome editing in bacterial plant pathogens without donor DNA templates.

PLoS Pathogens, 2023
CRISPR-based genome editing technology is revolutionizing prokaryotic research, but it has been rarely studied in bacterial plant pathogens. Here, we have developed a targeted genome editing method with no requirement of donor templates for convenient ...
Fang Yan, Jingwen Wang, Sujie Zhang, Zhenwan Lu, Shaofang Li, Zhiyuan Ji, Congfeng Song, Gongyou Chen, Jin Xu, Jie Feng, Xueping Zhou, Huanbin Zhou   +11 more
doaj   +1 more source

Assembling the perfect bacterial genome using Oxford Nanopore and Illumina sequencing.

PLoS Computational Biology, 2023
A perfect bacterial genome assembly is one where the assembled sequence is an exact match for the organism's genome-each replicon sequence is complete and contains no errors. While this has been difficult to achieve in the past, improvements in long-read
Ryan R Wick, Louise M Judd, Kathryn E Holt   +2 more
doaj   +2 more sources

One bacterial cell, one complete genome. [PDF]

PLoS ONE, 2010
While the bulk of the finished microbial genomes sequenced to date are derived from cultured bacterial and archaeal representatives, the vast majority of microorganisms elude current culturing attempts, severely limiting the ability to recover complete ...
Tanja Woyke, Damon Tighe, Konstantinos Mavromatis, Alicia Clum, Alex Copeland, Wendy Schackwitz, Alla Lapidus, Dongying Wu, John P McCutcheon, Bradon R McDonald, Nancy A Moran, James Bristow, Jan-Fang Cheng   +12 more
doaj   +1 more source

Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus

Frontiers in Microbiology, 2018
Staphylococcus aureus is a major human pathogen with well-characterized bacteriophage contributions to its virulence potential. Recently, we identified plasmidial and episomal prophages in S.
Douglas R. Deutsch, Bryan Utter, Kathleen J. Verratti, Heike Sichtig, Luke J. Tallon, Vincent A. Fischetti   +5 more
doaj   +1 more source

B-assembler: a circular bacterial genome assembler

BMC Genomics, 2022
Background Accurate bacteria genome de novo assembly is fundamental to understand the evolution and pathogenesis of new bacteria species. The advent and popularity of Third-Generation Sequencing (TGS) enables assembly of bacteria genomes at an ...
Fengyuan Huang, Li Xiao, Min Gao, Ethan J. Vallely, Kevin Dybvig, T. Prescott Atkinson, Ken B. Waites, Zechen Chong   +7 more
doaj   +1 more source

Complex Class 1 Integron in a Clinical Escherichia coli Strain From Vietnam Carrying Both mcr-1 and blaNDM–1

Frontiers in Microbiology, 2019
The co-production of MCR and carbapenemase in Enterobacteriaceae has been previously reported. Here, we describe a clinical strain of Escherichia coli from Vietnam carrying both mcr-1 and blaNDM–1.
Hong-Ngoc Le-Vo, Phuong Thi-Bich Tran, Lien Le, Yuki Matsumoto, Daisuke Motooka, Shota Nakamura, James W. Jones, Tetsuya Iida, Tetsuya Iida, Van Cao   +9 more
doaj   +1 more source

Quantitative Proteome Profiling of Coxiella burnetii Reveals Major Metabolic and Stress Differences Under Axenic and Cell Culture Cultivation

Frontiers in Microbiology, 2019
Coxiella burnetii is the causative agent of the zoonotic disease Q fever. To date, the lipopolysaccharide (LPS) is the only defined and characterized virulence determinant of C. burnetii. In this study, proteome profiles of C. burnetii Nine Mile phase I (
Jiri Dresler, Jana Klimentova, Petr Pajer, Barbora Salovska, Alena Myslivcova Fucikova, Martin Chmel, Gernot Schmoock, Heinrich Neubauer, Katja Mertens-Scholz   +8 more
doaj   +1 more source

Consistent Metagenome-Derived Metrics Verify and Delineate Bacterial Species Boundaries

mSystems, 2020
Longstanding questions relate to the existence of naturally distinct bacterial species and genetic approaches to distinguish them. Bacterial genomes in public databases form distinct groups, but these databases are subject to isolation and deposition ...
Matthew R. Olm, Alexander Crits-Christoph, Spencer Diamond, Adi Lavy, Paula B. Matheus Carnevali, Jillian F. Banfield   +5 more
doaj   +3 more sources

The Most Frequently Used Sequencing Technologies and Assembly Methods in Different Time Segments of the Bacterial Surveillance and RefSeq Genome Databases

Frontiers in Cellular and Infection Microbiology, 2020
Whole genome sequencing has become a powerful tool in modern microbiology. Especially bacterial genomes are sequenced in high numbers. Whole genome sequencing is not only used in research projects, but also in surveillance projects and outbreak ...
Bo Segerman, Bo Segerman
doaj   +1 more source

Genome-wide detection of spontaneous chromosomal rearrangements in bacteria. [PDF]

PLoS ONE, 2012
Genome rearrangements have important effects on bacterial phenotypes and influence the evolution of bacterial genomes. Conventional strategies for characterizing rearrangements in bacterial genomes rely on comparisons of sequenced genomes from related ...
Song Sun, Rongqin Ke, Diarmaid Hughes, Mats Nilsson, Dan I Andersson   +4 more
doaj   +1 more source