Results 81 to 90 of about 717,916 (306)
Pandora: nucleotide-resolution bacterial pan-genomics with reference graphs
Genome Biology, 2021 We present pandora, a novel pan-genome graph structure and algorithms for identifying variants across the full bacterial pan-genome. As much bacterial adaptability hinges on the accessory genome, methods which analyze SNPs in just the core genome have ...
Rachel M. Colquhoun +10 more
doaj +1 more source
Genome editing technologies to fight infectious diseases [PDF]
, 2017 Genome editing by programmable nucleases represents a promising tool that could be exploited to develop new therapeutic strategies to fight infectious diseases.
Barzon, Luisa +2 more
core +1 more source
Clades of huge phages from across Earth's ecosystems. [PDF]
, 2020 Bacteriophages typically have small genomes1 and depend on their bacterial hosts for replication2. Here we sequenced DNA from diverse ecosystems and found hundreds of phage genomes with lengths of more than 200 kilobases (kb), including a genome of ...
Al-Shayeb, Basem +44 more
core +2 more sources
Molecular Oncology, EarlyView.TGF‐β has a complex role in cancer, exhibiting both tumor‐suppressive and tumor‐promoting properties. Using a series of differentiated tumoroids, derived from different stages and mutational background of colorectal cancer patients, we replicate this duality of TGF‐β in vitro. Notably, the atypical but highly aggressive KRASQ22K mutation rendered early‐
Theresia Mair +17 more
wiley +1 more source
An Important Role for Purifying Selection in Archaeal Genome Evolution
mSystems, 2017 As the null hypothesis of genome evolution, population genetic theory suggests that selection strength controls genome size. Through the process of genetic drift, this theory predicts that compact genomes are maintained by strong purifying selection ...
Zhe Lyu +3 more
doaj +1 more source
Efficient dual-negative selection for bacterial genome editing
BMC Microbiology, 2020 Background Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes.
Francesca Romana Cianfanelli +2 more
doaj +1 more source
Quantifying the common genetic variability of bacterial traits [PDF]
arXiv, 2023 The study of common heritability, or co-heritability, among multiple traits has been widely established in quantitative and molecular genetics. However, in bacteria, genome-based estimation of heritability has only been considered very recently and no methods are currently available for considering co-heritability.
arxiv
Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence
Molecular Oncology, EarlyView.The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova +18 more
wiley +1 more source
Visualization of ribosomal RNA operon copy number distribution
BMC Microbiology, 2009 Background Results of microbial ecology studies using 16S rRNA sequence information can be deceiving due to differences in rRNA operon copy number and genome size of the detected organisms.
DasGupta Indrani +3 more
doaj +1 more source
Defining the estimated core genome of bacterial populations using a Bayesian decision model. [PDF]
PLoS Computational Biology, 2014 The bacterial core genome is of intense interest and the volume of whole genome sequence data in the public domain available to investigate it has increased dramatically.
Andries J van Tonder +12 more
doaj +1 more source