Results 101 to 110 of about 2,805,364 (358)

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

An Important Role for Purifying Selection in Archaeal Genome Evolution

mSystems, 2017
As the null hypothesis of genome evolution, population genetic theory suggests that selection strength controls genome size. Through the process of genetic drift, this theory predicts that compact genomes are maintained by strong purifying selection ...
Zhe Lyu, Zhi-Gang Li, Fei He, Ziding Zhang   +3 more
doaj   +1 more source

Pandora: nucleotide-resolution bacterial pan-genomics with reference graphs

Genome Biology, 2021
We present pandora, a novel pan-genome graph structure and algorithms for identifying variants across the full bacterial pan-genome. As much bacterial adaptability hinges on the accessory genome, methods which analyze SNPs in just the core genome have ...
Rachel M. Colquhoun, Michael B. Hall, Leandro Lima, Leah W. Roberts, Kerri M. Malone, Martin Hunt, Brice Letcher, Jane Hawkey, Sophie George, Louise Pankhurst, Zamin Iqbal   +10 more
doaj   +1 more source

Chemical synthesis rewriting of a bacterial genome to achieve design flexibility and biological functionality

Proceedings of the National Academy of Sciences of the United States of America, 2019
Significance The fundamental biological functions of a living cell are stored within the DNA sequence of its genome. Classical genetic approaches dissect the functioning of biological systems by analyzing individual genes, yet uncovering the essential ...
Jonathan Venetz, Luca Del Medico, A. Wölfle, Philipp Schächle, Yves Bucher, Donat Appert, Flavia Tschan, C. E. Flores-Tinoco, Mariëlle J. F. M. van Kooten, Rym Guennoun, S. Deutsch, Matthias Christen, Beat Christen   +12 more
semanticscholar   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source

Genetic Diversity and Spatial Segregation of Francisella tularensis Subspecies holarctica in Germany

Frontiers in Cellular and Infection Microbiology, 2019
Francisella tularensis is an intracellular pleomorphic bacterium and the causative agent of tularemia, a zoonotic disease with a wide host range. Among the F. tularensis subspecies, especially F. tularensis subsp.
Sandra Appelt, Kristin Köppen, Aleksandar Radonić, Oliver Drechsel, Daniela Jacob, Roland Grunow, Klaus Heuner   +6 more
doaj   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Visualization of ribosomal RNA operon copy number distribution

BMC Microbiology, 2009
Background Results of microbial ecology studies using 16S rRNA sequence information can be deceiving due to differences in rRNA operon copy number and genome size of the detected organisms.
DasGupta Indrani, Wu Martin, Rastogi Rajat, Fox George E   +3 more
doaj   +1 more source

Efficient dual-negative selection for bacterial genome editing

BMC Microbiology, 2020
Background Gene editing is key for elucidating gene function. Traditional methods, such as consecutive single-crossovers, have been widely used to modify bacterial genomes.
Francesca Romana Cianfanelli, Olivier Cunrath, Dirk Bumann   +2 more
doaj   +1 more source

Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola [PDF]

, 2009
Submarine hydrothermal vents are model systems for the Archaean Earth environment, and some sites maintain conditions that may have favored the formation and evolution of cellular life.
Robinson, Jeffrey M, Campbell, Barbara J, Hoda M Khouri, S. Craig Cary, Hanson, Thomas E., Lee Charles K., Jonathan A. Eisen, Wu, Dongying, Eisen, Jonathan A., Barbara J Campbell, Jeffrey M Robinson, Cary, S Craig, Robinson, Jeffrey M., Charles K. Lee, Stein, Lisa Y., Lisa Y Stein, Stein, Lisa Y, Julie L Smith, Thomas E Hanson, Klotz, Martin G., Cary S. Craig, Eisen, Jonathan A, Khouri Hoda M., Khouri, Hoda M, Hanson, Thomas E, Hanson Thomas E., Eisen Jonathan A., Thomas E. Hanson, Dongying Wu, S Craig Cary, Jonathan A Eisen, Lee, Charles K, Smith, Julie L., Klotz Martin G., Cary, S. Craig, Wu Dongying, Charles K Lee, Smith Julie L., Martin G. Klotz, Campbell, Barbara J., Klotz, Martin G, Campbell Barbara J., Barbara J. Campbell, Julie L. Smith, Lee, Charles Kai-Wu, Khouri, Hoda M., Smith, Julie L, Lisa Y. Stein, Jeffrey M. Robinson, Hoda M. Khouri, Martin G Klotz, Stein Lisa Y., Robinson Jeffrey M.   +52 more
core   +1 more source