Results 81 to 90 of about 772,345 (307)

In vitro and in vivo screening for novel essential cell-envelope proteins in Pseudomonas aeruginosa [PDF]

, 2015
The Gram-negative bacterium Pseudomonas aeruginosa represents a prototype of multi-drug resistant opportunistic pathogens for which novel therapeutic options are urgently required.
Bragonzi, Alessandra, Fernandez Pinar, Regina, Imperi, Francesco, LO SCIUTO, Alessandra, Ranucci, Serena, Rossi, Alice   +5 more
core   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

FEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane, Lea Nikolić, Lorenzo Maraio, Thomas Goiran, Nathan Karpilovsky, Stefano Sellitto, Vangelis Bouris, Jiang‐An Yin, Ronald Melki, Edward A Fon, Adriano Aguzzi, Elena De Cecco   +11 more
wiley   +1 more source

Visualization of ribosomal RNA operon copy number distribution

BMC Microbiology, 2009
Background Results of microbial ecology studies using 16S rRNA sequence information can be deceiving due to differences in rRNA operon copy number and genome size of the detected organisms.
DasGupta Indrani, Wu Martin, Rastogi Rajat, Fox George E   +3 more
doaj   +1 more source

Identifying transcription factors controlling the basal expression of human MRP4 highlights a substantial role for Sp1

FEBS Open Bio, EarlyView.
The MRP4 transporter exports several drugs and signaling molecules. Here, we identified key promoter elements regulating basal MRP4 expression. Using reporter assays, we defined a conserved region with essential Sp1 and contributory Ets sites, which controlled basal MRP4 expression.
Debora Singer, Angelique Kragl, Katrin Ziems, Juliane Glaubitz, Sophie Grammbauer, Susanne Hildebrandt, Mladen V. Tzvetkov, Gabriele Jedlitschky   +7 more
wiley   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Circlator: automated circularization of genome assemblies using long sequencing reads [PDF]

, 2015
The assembly of DNA sequence data is undergoing a renaissance thanks to emerging technologies capable of producing reads tens of kilobases long.
Harris, Simon R., Hunt, Martin, Keane, Jacqueline A., Otto, Thomas D., Parkhill, Julian, Silva, Nishadi De   +5 more
core   +3 more sources

Biomimetic Copper Nanoparticles Coated with ACE2‐Overexpressing Membranes for Selective SARS‐CoV‐2 Neutralization and Disinfection

Advanced Healthcare Materials, EarlyView.
By fusing ACE2‐overexpressing membrane‐integrated liposome (MIL) with copper nanoparticles, the biomimetic Cu@MIL nanostructures were created that directly hijack the SARS‐CoV‐2 entry pathway. These particles combine potent virus‐targeting precision with intrinsic antiviral activity, achieving rapid neutralization and disinfection.
Pooja Aich, Wei‐Pang Chung, Wen‐Jyun Wang, Hidenobu Takahashi, Ming‐Shun Hsieh, Ying‐Chen Lin, Oh Seok Kwon, Wei‐Peng Li, Wei‐Lun Huang   +8 more
wiley   +1 more source

What does the talking?: quorum sensing signalling genes discovered in a bacteriophage genome. [PDF]

PLoS ONE, 2014
The transfer of novel genetic material into the genomes of bacterial viruses (phages) has been widely documented in several host-phage systems. Bacterial genes are incorporated into the phage genome and, if retained, subsequently evolve within them.
Katherine R Hargreaves, Andrew M Kropinski, Martha R J Clokie   +2 more
doaj   +1 more source

Microbial genomic taxonomy [PDF]

, 2013
A need for a genomic species definition is emerging from several independent studies worldwide. In this commentary paper, we discuss recent studies on the genomic taxonomy of diverse microbial groups and a unified species definition based on genomics ...
Chimetto, Luciane, Edwards, Robert A, Stackebrandt, Erko, Swings, Jean, Thompson, Cristiane C, Thompson, Fabiano L   +5 more
core   +3 more sources

Positional Homology in Bacterial Genomes [PDF]

Evolutionary Bioinformatics, 2006
In comparative genomic studies, syntenic groups of homologous sequence in the same order have been used as supplementary information that can be used in helping to determine the orthology of the compared sequences. The assumption is that orthologous gene copies are more likely to share the same genome positions and share the same gene neighbors.
Ingrid J. Burgetz, Salimah Shariff, Andy Pang, Elisabeth R. M. Tillier*   +3 more
openaire   +4 more sources