Results 81 to 90 of about 99 (98)
Is the endophyte‐based plant protection against aphids mediated by changes in the insect microbiome?
We showed that the fungal endophyte Epichloë strain AR37 increased the plant resistance against aphids via the production of indole diterpene alkaloids. The reduction in aphid performance was not associated with changes in abundance/composition/diversity of the insect's bacterial microbiota, but additional endophyte effects on this microbiota cannot be
Daniel A. Bastías+5 more
wiley +1 more source
Humanimals: A Socio‐Ecological Reading of the Marseille Plague of 1720
Abstract The aim of this article is to return to a small number of historically significant first‐person testimonies of the Marseille epidemic of 1720 in order to analyse in detail their construction and depiction of human exceptionality as a form of life in a time of plague.
David McCallam
wiley +1 more source
ABSTRACT The condition of ancient marine ecosystems provides context for contemporary biodiversity changes in human‐impacted oceans. Sequencing sedimentary ancient DNA (sedaDNA) is an emerging method for generating high‐resolution biodiversity time‐series data, offering insights into past ecosystems.
Luke E. Holman+8 more
wiley +1 more source
ABSTRACT Amplicon sequencing tools such as metabarcoding are commonly used for thorough characterisation of microbial diversity in natural samples. They mostly rely on the amplification of conserved universal markers, mainly ribosomal genes, allowing the taxonomic assignment of barcodes.
Silke Van den Wyngaert+21 more
wiley +1 more source
ABSTRACT Viral diseases represent a threat to global food production. Managing the impact of viruses on crop production requires the ability to monitor viruses, study their ecology and anticipate outbreaks. Double‐stranded RNA (dsRNA) sequencing is a well‐established and reliable method of detecting viruses and studying virome‐host interactions and ...
Mamadou L. Fall+5 more
wiley +1 more source
CCS‐Consensuser: A Haplotype‐Aware Consensus Generator for PacBio Amplicon Sequences
ABSTRACT DNA sequencing technology has undergone substantial improvements in recent years, to the extent that Third Generation Sequencing platforms are capable of massively generating long‐reads. Amplicon sequencing has been among the most popular techniques due to its wide application in diverse fields of biological sciences.
Carlos Congrains+10 more
wiley +1 more source
Observation Bias in Metabarcoding
ABSTRACT DNA metabarcoding is subject to observation bias associated with PCR and sequencing, which can result in observed read proportions differing from actual species proportions in the DNA extract. Here, we amplify and sequence a mock community of known composition containing marine fishes and cetaceans using four different primer sets and a ...
Megan R. Shaffer+5 more
wiley +1 more source
ABSTRACT Numerous specimens stored in natural history collections have been involuntarily preserved together with their associated microbiomes. We propose exploiting century‐old soils occasionally found on the roots of herbarium plants to assess the diversity of ancient soil microbial communities originally associated with these plants.
Gianluca Grasso+7 more
wiley +1 more source
Abstract Phylogenetic trees are increasingly used to infer the processes that shape biodiversity patterns, such as diversification, dispersal and trait evolution. However, collecting many samples and sequencing them for phylogenetic reconstruction is challenging and costly, and achieving high sampling fractions can be logistically impossible for ...
Ornela N. Dehayem+4 more
wiley +1 more source
We show that genetic and pharmacological inhibition of the metabotropic glutamate receptor, DmGluRA extends Drosophila lifespan, improves stress tolerance and alters early‐life locomotion. Several markers indicative of reduced ribosomal biogenesis, a hallmark of longevity, were also detected in these flies.
Cui Guan+4 more
wiley +1 more source