Results 111 to 120 of about 2,630,320 (343)

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

FEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point, Wafaa Achache, Janïs Laudouze, Eliana Sepulveda Ramos, Mickaël Maziero, Céline Crauste, Stéphane Canaan, Pierre Santucci   +7 more
wiley   +1 more source

Towards Viral Genome Annotation Standards, Report from the 2010 NCBI Annotation Workshop

Viruses, 2010
Improvements in DNA sequencing technologies portend a new era in virology and could possibly lead to a giant leap in our understanding of viral evolution and ecology.
Qiandong Zeng, William Klimke, Peter Sterk, Susmita Shrivastava, Ramana Madupu, Richard H. Scheuermann, Seth Schobel, Donald Seto, Yiming Bao, Carla Kuiken, Elliot J. Lefkowitz, Philippe Le Mercier, Raphael Leplae, Tatiana Tatusova, James Rodney Brister   +14 more
doaj   +1 more source

Natural resistance of the diploid Musa balbisiana Pisang Klutuk Wulung (PKW) to banana streak virus is probably driven by transcriptional gene silencing [PDF]

, 2013
The genome of banana (Musa sp.) harbours multiple integrations of Banana streak virus (eBSV), whereas this badnavirus does not require integration for the replication of its ds DNA genome.
Chabannes, Matthieu, Duroy, Pierre-Olivier, Iskra Caruana, Marie-Line, Laboureau, Nathalie, Pooggin, Mikhail, Rajendran, Rajeswaran, Seguin, Jonathan   +6 more
core  

dUTPase is essential in zebrafish development and possesses several single‐nucleotide variants with pronounced structural and functional consequences

FEBS Open Bio, EarlyView.
dUTPases are involved in balancing the appropriate nucleotide pools. We showed that dUTPase is essential for normal development in zebrafish. The different zebrafish genomes contain several single‐nucleotide variations (SNPs) of the dut gene. One of the dUTPase variants displayed drastically lower protein stability and catalytic efficiency as compared ...
Viktória Perey‐Simon, Angéla Békesi, Latifa Kazzazy, Jázmin Mihály, Máté Varga, Beáta G. Vértessy, Kinga Nyíri   +6 more
wiley   +1 more source

A new role for human papillomavirus 16 E2: Mitotic activation of the DNA damage response to promote viral genome segregation

Tumour Virus Research
Human papillomaviruses (HPV) are causative agents in around 5% of all human cancers. To identify and develop new targeted HPV therapeutics we must enhance our understanding of the viral life cycle and how it interacts with the host.
Apurva T. Prabhakar, Iain M. Morgan
doaj   +1 more source

Persistence of an Oncogenic Papillomavirus Genome Requires cis Elements from the Viral Transcriptional Enhancer

mBio, 2017
Human papillomavirus (HPV) genomes are replicated and maintained as extrachromosomal plasmids during persistent infection. The viral E2 proteins are thought to promote stable maintenance replication by tethering the viral DNA to host chromatin.
Koenraad Van Doorslaer, Dan Chen, Sandra Chapman, Jameela Khan, Alison A. McBride   +4 more
doaj   +1 more source

A 212-nt long RNA structure in the Tobacco necrosis virus-D RNA genome is resistant to Xrn degradation [PDF]

, 2019
Plus-strand RNA viruses can accumulate viral RNA degradation products during infections. Some of these decay intermediates are generated by the cytosolic 5′-to-3′ exoribonuclease Xrn1 (mammals and yeast) or Xrn4 (plants) and are formed when the enzyme ...
Chaminda, Gunawardene D., Newburn, Laura R., White, K. Andrew   +2 more
core   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

ATRX restricts Human Cytomegalovirus (HCMV) viral DNA replication through heterochromatinization and minimizes unpackaged viral genomes.

PLoS Pathogens
ATRX limits the accumulation of human cytomegalovirus (HCMV) Immediate Early (IE) proteins at the start of productive, lytic infections, and thus is a part of the cell-intrinsic defenses against infecting viruses.
Ryan M Walter, Kinjal Majumder, Robert F Kalejta   +2 more
doaj   +1 more source

Endogenous banana streak virus sequences (eBSV) are likely transcriptionally silenced in the resistant seedy diploid Musa balbisiana Pisang Klutuk Wulung (PKW). [P.50] [PDF]

, 2015
The B genome of banana (Musa sp.) harbours integrations of Banana streak virus (eBSV) for at least three BSV species, whereas this badnavirus does not require integration for the replication of its ds DNA genome.
Caruana, Marie-Line, Chabannes, Matthieu, Duroy, Pierre-Olivier, Laboureau, Nathalie, Pooggin, Mikhail, Rajendran, Rajeswaran, Seguin, Jonathan   +6 more
core