Results 141 to 150 of about 4,524,973 (357)

Improved vectors and genome-wide libraries for CRISPR screening

Nature Methods, 2014
Genome-wide, targeted loss-of-function pooled screens using the CRISPR (clustered regularly interspaced short palindrome repeats)–associated nuclease Cas9 in human and mouse cells provide an alternative screening system to RNA interference (RNAi) and ...
Neville E. Sanjana, Ophir Shalem, Feng Zhang   +2 more
semanticscholar   +1 more source

Targeted modulation of IGFL2‐AS1 reveals its translational potential in cervical adenocarcinoma

Molecular Oncology, EarlyView.
Cervical adenocarcinoma patients face worse outcomes than squamous cell carcinoma counterparts despite similar treatment. The identification of IGFL2‐AS1's differential expression provides a molecular basis for distinguishing these histotypes, paving the way for personalized therapies and improved survival in vulnerable populations globally.
Ricardo Cesar Cintra, Lucca Paolo Hsu Helmich, Daniel Rodrigues de Bastos, Laura Sichero, Patrícia Savio de Araujo‐Souza, Fabio Passetti, Luisa Lina Villa   +6 more
wiley   +1 more source

Genomic Insights and Comparative Analysis of Novel Rhodopseudomonas Species: A Purple Non-Sulfur Bacterium Isolated from Latex Rubber Sheet Wastewater

Life
Rhodopseudomonas is recognized for its versatile metabolic capabilities that enable it to effectively degrade pollutants and survive various environmental stresses. In this study, we conducted a genome analysis of Rhodopseudomonas sp.
Chollachai Klaysubun, Nattarika Chaichana, Sirikan Suwannasin, Kamonnut Singkhamanan, Thunchanok Yaikhan, Duangporn Kantachote, Rattanaruji Pomwised, Monwadee Wonglapsuwan, Komwit Surachat   +8 more
doaj   +1 more source

siRNA Genome Screening Approaches to Therapeutic Drug Repositioning

Pharmaceuticals, 2013
Bridging high-throughput screening (HTS) with RNA interference (RNAi) has allowed for rapid discovery of the molecular basis of many diseases, and identification of potential pathways for developing safe and effective treatments.
Ralph A. Tripp, S. Mark Tompkins, Olivia Perwitasari, Abhijeet Bakre   +3 more
doaj   +1 more source

novoBreak: local assembly for breakpoint detection in cancer genomes. [PDF]

, 2017
We present novoBreak, a genome-wide local assembly algorithm that discovers somatic and germline structural variation breakpoints in whole-genome sequencing data.
Boutros, Paul, Chen, Junjie, Chen, Ken, Chen, Tenghui, Chong, Zechen, Ding, Li, Fan, Xian, Gao, Min, Lee, Anna Y, Ruan, Jue, Zhou, Wanding   +10 more
core  

Network divergence analysis identifies adaptive gene modules and two orthogonal vulnerability axes in pancreatic cancer

Molecular Oncology, EarlyView.
Tumors contain diverse cellular states whose behavior is shaped by context‐dependent gene coordination. By comparing gene–gene relationships across biological contexts, we identify adaptive transcriptional modules that reorganize into distinct vulnerability axes.
Brian Nelson, Lyanne Delgado‐Coka, Natalia Marchenko, Luisa F. Escobar‐Hoyos, Kenneth R. Shroyer, Alisa Yurovsky, Trey Ideker, Gábor Balázsi, Thomas MacCarthy, Scott Powers   +9 more
wiley   +1 more source

Drug Susceptibility, Siderophore Production, and Genome Analysis of Staphylococcus aureus Clinical Isolates from a University Hospital in Chiang Mai, Thailand

Antibiotics
Background/Objective:Staphylococcus aureus produces staphyloferrin A (Sfna) siderophores to sequester host iron during infection and rapid cell proliferation We examined drug susceptibility, siderophore production, and genome sequencing of clinical ...
Warinda Prommachote, Manu Deeudom, Pimpisid Koonyosying, Phronpawee Srichomphoo, Ratchanee Somnabut, Phadungkiat Khamnoi, Agostino Cilibrizzi, Yuvaraj Ravikumar, Somdet Srichairatanakool   +8 more
doaj   +1 more source

RIPK4 function interferes with melanoma cell adhesion and metastasis

Molecular Oncology, EarlyView.
RIPK4 promotes melanoma growth and spread. RIPK4 levels increase as skin lesions progress to melanoma. CRISPR/Cas9‐mediated deletion of RIPK4 causes melanoma cells to form less compact spheroids, reduces their migratory and invasive abilities and limits tumour growth and dissemination in mouse models.
Norbert Wronski, Sławomir Lasota, Ewelina Madej, Anna A. Brożyna, Małgorzata Szczygieł, Agnieszka Harazin‐Lechowska, Jan Czerbniak, Janusz Rys, Jaroslaw Czyz, Agnieszka Wolnicka‐Glubisz   +9 more
wiley   +1 more source

Pantoea stewartii subsp. stewartii an Inter-Laboratory Comparative Study of Molecular Tests and Comparative Genome Analysis of Italian Strains

Plants
Pantoea stewartii subsp. stewartii (Pss) is a Gram-negative bacterium causing Stewart wilt, a severe disease in maize. Native to North America, it has spread globally through the maize seed trade. Resistant maize varieties and insecticides are crucial to
Valeria Scala, Nicoletta Pucci, Riccardo Fiorani, Alessia L’Aurora, Alessandro Polito, Marco Di Marsico, Riccardo Aiese Cigliano, Eleonora Barra, Serena Ciarroni, Francesca De Amicis, Salvatore Fascella, Francesca Gaffuri, Andreas Gallmetzer, Francesca Giacobbi, Pasquale Domenico Grieco, Valeria Gualandri, Giovanna Mason, Daniela Pasqua di Bisceglie, Domenico Rizzo, Maria Rosaria Silletti, Simona Talevi, Marco Testa, Cosimo Tocci, Stefania Loreti   +23 more
doaj   +1 more source

Pre‐analytical optimization of cell‐free DNA and extracellular vesicle‐derived DNA for mutation detection in liquid biopsies

Molecular Oncology, EarlyView.
Pre‐analytical handling critically determines liquid biopsy performance. This study defines practical best‐practice conditions for cell‐free DNA (cfDNA) and extracellular vesicle–derived DNA (evDNA), showing how processing time, storage conditions, tube type, and plasma input volume affect DNA integrity and mutation detection.
Jonas Dohmen, Lucrezia De Santis, Bret M. Stephens, Vincent Bernard, Jörg C. Kalff, Lara Braun, Phillipp Leyendecker, Johannes Röttgen, Daniel Weissinger, Anirban Maitra, Paola A. Guerrero, Alexander Semaan   +11 more
wiley   +1 more source