Expanding CRISPR/Cas9 Genome Editing Capacity in Zebrafish Using SaCas9. [PDF]
, 2016 The type II CRISPR/Cas9 system has been used widely for genome editing in zebrafish. However, the requirement for the 5'-NGG-3' protospacer-adjacent motif (PAM) of Cas9 from Streptococcus pyogenes (SpCas9) limits its targeting sequences.
Chen, Cheng +8 more
core +4 more sources
Collection of prokaryotic genome contents expectation rules from scientific literature [PDF]
arXiv, 2023 Shaped by natural selection and other evolutionary forces, an organism's evolutionary history is reflected through its genome sequence, content of functional elements and organization. Consequently, organisms connected through phylogeny, metabolic or morphological traits, geographical proximity, or habitat features are likely to exhibit similarities in
arxiv
Improved genome editing in human cell lines using the CRISPR method [PDF]
, 2014 The Cas9/CRISPR system has become a popular choice for genome editing. In this system, binding of a single guide (sg) RNA to a cognate genomic sequence enables the Cas9 nuclease to induce a double-strand break at that locus.
AW Briggs +22 more
core +8 more sources
Genome editing in non-model organisms opens new horizons for comparative physiology [PDF]
, 2020 For almost 100 years, biologists have made fundamental discoveries using a handful of model organisms that are not representative of the rich diversity found in nature.
Dickinson, Michael H. +2 more
core
Switchable genome editing via genetic code expansion [PDF]
, 2018 Multiple applications of genome editing by CRISPR-Cas9 necessitate stringent regulation and Cas9 variants have accordingly been generated whose activity responds to small ligands, temperature or light.
Asami, Maki +5 more
core +2 more sources
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Genome Biology, 2018 Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at ...
Chao Li +7 more
doaj +1 more source
Scientific Reports, 2022 Cyclin-dependent kinases (CDKs) control the progression of the cell cycle. D-type cyclin (CYCD) is generally believed to form a complex with CDK and control the G1/S transition.
Chengcheng He +5 more
doaj +1 more source
CAS9 is a genome mutator by directly disrupting DNA-PK dependent DNA repair pathway. [PDF]
, 2020 With its high efficiency for site-specific genome editing and easy manipulation, the clustered regularly interspaced short palindromic repeats (CRISPR)/ CRISPR associated protein 9 (CAS9) system has become the most widely used gene editing technology in ...
Chen, Qu +6 more
core
Genome Editing with Crispr-Cas9 Systems: Basic Research and Clinical Applications [PDF]
, 2017 BACKGROUND: Recently established genome editing technologies will open new avenues for biological research and development. Human genome editing is a powerful tool which offers great scientific and therapeutic potential.CONTENT: Genome editing using the ...
Dewi, N. M. (Nurrani) +2 more
core +4 more sources
The role of APOBEC3-induced mutations in the differential evolution of monkeypox virus [PDF]
arXiv, 2023 Recent studies show that newly sampled monkeypox virus (MPXV) genomes exhibit mutations consistent with Apolipoprotein B mRNA Editing Catalytic Polypeptide-like3 (APOBEC3)-mediated editing, compared to MPXV genomes collected earlier. It is unclear whether these single nucleotide polymorphisms (SNPs) result from APOBEC3-induced editing or are a ...
arxiv