Results 141 to 150 of about 571,422 (359)

USDA regulation of organisms developed through modern breeding technologies [PDF]

, 2014
Agricultural biotechnology; DNA editing; genome engineering; targetable nucleases; TALEN; CRISPR/cas9; DNA-targeting; mutagenesis; regulation; modern breeding ...
Hoffman, Neil E.
core  

267. Highly-Efficient Genome Editing in Human Stem Cells Using Engineered Zinc Finger Nucleases [PDF]

, 2008

openalex   +1 more source

NMR screening of low molecular weight inhibitors targeting the papain‐like protease (PLPro) of SARS‐CoV‐2

FEBS Open Bio, EarlyView.
Papain‐like protease from SARS‐CoV‐2 plays an important role in the cleavage of the viral polyproteins and in the suppression of the host's immune response. Here, we present the results of an NMR screening study. We identified 86 binding compounds, of which five candidates were chosen for in‐depth analysis.
Dennis J. Pyper, Sridhar Sreeramulu, Benjamin T. Lanham, Elizabeth M. Engle, David Fushman, Harald Schwalbe   +5 more
wiley   +1 more source

Increasing Gene Editing Efficiency via CRISPR/Cas9- or Cas12a-Mediated Knock-In in Primary Human T Cells

Biomedicines
T lymphocytes represent a promising target for genome editing. They are primarily modified to recognize and kill tumor cells or to withstand HIV infection. In most studies, T cell genome editing is performed using the CRISPR/Cas technology. Although this
Natalia Kruglova, Mikhail Shepelev
doaj   +1 more source

Embryos as Patients? Medical Provider Duties in the Age of CRISPR/Cas9 [PDF]

, 2017
The CRISPR/Cas9 genome engineering platform is the first method of gene editing that could potentially be used to treat genetic disorders in human embryos. No past therapies, genetic or otherwise, have been intended or used to treat disorders in existent
Powell, G. Edward, III
core   +1 more source

Genome editing in non-model organisms opens new horizons for comparative physiology [PDF]

, 2020
For almost 100 years, biologists have made fundamental discoveries using a handful of model organisms that are not representative of the rich diversity found in nature.
Dickinson, Michael H., Dow, Julian A. T., Vosshall, Leslie B.   +2 more
core  

Large-scale DNA editing of retrotransposons accelerates mammalian genome evolution [PDF]

, 2011
Shai Carmi, George M. Church, Erez Y. Levanon   +2 more
openalex   +1 more source

Actin dynamics controlled by IqgC, a RasGAP at the crossroads between the IQGAP and fungal GAP1 families

FEBS Open Bio, EarlyView.
IqgC is a RasGAP from Dictyostelium discoideum. IqgC binds RasG via its RasGAP domain and deactivates it on macroendocytic cups, thereby suppressing the uptake of fluid and particles. IqgC has a positive effect on cell‐substratum adhesion, and its RGCt domain is required for recruitment to ventral foci.
Vedrana Filić, Darija Putar, Lucija Mijanović, Igor Weber   +3 more
wiley   +1 more source

Cas9+ conditionally-immortalized macrophages as a tool for bacterial pathogenesis and beyond. [PDF]

, 2019
Macrophages play critical roles in immunity, development, tissue repair, and cancer, but studies of their function have been hampered by poorly-differentiated tumor cell lines and genetically-intractable primary cells.
Barton, Gregory M, Ching, Krystal L, Cox, Jeffery S, Golovkine, Guillaume, Licht, Daniel J, Mitchell, Gabriel, Popov, Lauren M, Roberts, Allison W   +7 more
core  

Novel reporter systems for facile evaluation of I-SceI-mediated genome editing [PDF]

, 2011
Nina M. Muñoz, Brian C. Beard, Byoung Y. Ryu, Ralf M. Luche, Grant D. Trobridge, David J. Rawlings, Andrew M. Scharenberg, Hans‐Peter Kiem   +7 more
openalex   +1 more source