Results 211 to 220 of about 1,963,187 (422)

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, EarlyView.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

267. Highly-Efficient Genome Editing in Human Stem Cells Using Engineered Zinc Finger Nucleases [PDF]

, 2008

openalex   +1 more source

Enhanced discovery of bacterial laccase‐like multicopper oxidase through computer simulation and metagenomic analysis of industrial wastewater

FEBS Open Bio, EarlyView.
We obtained potential bacterial laccase‐like multicopper oxidase (LMCO) sequences through metagenomic sequencing. All sequences exhibited significant differences from known LMCOs in databases. To select the most promising candidates, we performed structure prediction and molecular docking using alphafold2, metal3d and rosetta.
Ting Cui, Ariel Kushmaro, Hana Barak, Anja Poehlein, Rolf Daniel, Hans‐Jürgen Mägert   +5 more
wiley   +1 more source

Lung Organoids from hiPSCs Can Be Efficiently Transduced by Recombinant Adeno-Associated Viral and Adenoviral Vectors

Biomedicines
Background: Organoids are a valuable model for studying hereditary diseases such as cystic fibrosis (CF). Recombinant adenoviral (rAdV) and adeno-associated viral (rAAV) vectors are promising tools for CF gene therapy and genome editing.
Lyubava Belova, Anna Demchenko, Anastasia Erofeeva, Konstantin Kochergin-Nikitsky, Olga Zubkova, Olga Popova, Tatiana Ozharovskaia, Diana Salikhova, Anna Efremova, Alexander Lavrov, Svetlana Smirnikhina   +10 more
doaj   +1 more source

Large-scale DNA editing of retrotransposons accelerates mammalian genome evolution [PDF]

, 2011
Shai Carmi, George M. Church, Erez Y. Levanon   +2 more
openalex   +1 more source

Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins

FEBS Open Bio, EarlyView.
With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet, Julia Bartoli, Atakan Kabtan, Chloé M. Piras, Audrey C. Tempier, Eric Cascales, Julie P. Viala   +6 more
wiley   +1 more source

A framework for cost-constrained genome rearrangement under Double Cut and Join [PDF]

arXiv, 2018
The study of genome rearrangement has many flavours, but they all are somehow tied to edit distances on variations of a multi-graph called the breakpoint graph. We study a weighted 2-break distance on Eulerian 2-edge-colored multi-graphs, which generalizes weighted versions of several Double Cut and Join problems, including those on genomes with ...
arxiv  

Novel reporter systems for facile evaluation of I-SceI-mediated genome editing [PDF]

, 2011
Nina M. Muñoz, Brian C. Beard, Byoung Y. Ryu, Ralf M. Luche, Grant D. Trobridge, David J. Rawlings, Andrew M. Scharenberg, Hans‐Peter Kiem   +7 more
openalex   +1 more source

Zinc finger nuclease (ZFN)-mediated genome editing to generate isogenic, pluripotent cell lines [PDF]

, 2011

openalex   +1 more source

Novel Phenotypes and Deep Intronic Variant Expand TH‐Associated Dopa‐Responsive Dystonia Spectrum

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Approximately 20% of dopa‐responsive dystonia (DRD) cases remain genetically unresolved. Using whole‐genome sequencing, we identified two TH variants in a young DRD patient, including a novel deep intronic variant. Minigene assays confirmed that this variant causes aberrant splicing.
Xiaosheng Zheng, Chenxin Ying, Nan Jin, Jinghong Ma, Xinhua Wan, Xunhua Li, Wei Luo   +6 more
wiley   +1 more source