Results 71 to 80 of about 473,506 (307)
PEM-seq comprehensively quantifies DNA repair outcomes during gene-editing and DSB repair
STAR Protocols, 2022 Summary: The repair products of double-stranded DNA breaks (DSBs) are crucial for investigating the mechanism underlying DNA damage repair as well as evaluating the safety and efficiency of gene-editing; however, a comprehensively quantitative assay ...
Yang Liu +6 more
doaj +1 more source
Molecular Oncology, EarlyView.Glioma cells mainly express the endothelin receptor EDNRB, while EDNRA is restricted to a perivascular tumor subpopulation. Endothelin signaling reduces glioma cell proliferation while promoting migration and a proneural‐to‐mesenchymal transition associated with poor prognosis. This pathway activates Ca2+, K+, ERK, and STAT3 signalings and is regulated
Donovan Pineau +36 more
wiley +1 more source
Frontiers in Genetics, 2020 The NETO2 gene (neuropilin and tolloid-like 2) encodes a protein that acts as an accessory subunit of kainate receptors and is predominantly expressed in the brain.
Maria S. Fedorova +14 more
doaj +1 more source
Interrogating the immune landscape of microsatellite stable RAS‐mutated colon cancer
Molecular Oncology, EarlyView.COLOSSUS project RAS‐mutated MSS colon cancer study explored transcriptomics and immune cell density by immunohistochemistry (IHC), Immunoscore (IS), ISIC/TuLIS scores, mutation counts, and detected different prevalences but similar microenvironment composition across immune markers with clinical relevance for future immunotherapy combination ...
Rodrigo Dienstmann +61 more
wiley +1 more source
Telomeres in Evolution and Development from Biosemiotic Perspective
, 2007 Telomeres identify natural chromosome ends being different from broken DNA through differences in their "molecular syntax" (M.Eigen) which determines the functions of reverse transcriptase and its integrated RNA template, telomerase.
Guenther Witzany
core +1 more source
Expanded base editing in rice and wheat using a Cas9-adenosine deaminase fusion
Genome Biology, 2018 Nucleotide base editors in plants have been limited to conversion of cytosine to thymine. Here, we describe a new plant adenine base editor based on an evolved tRNA adenosine deaminase fused to the nickase CRISPR/Cas9, enabling A•T to G•C conversion at ...
Chao Li +7 more
doaj +1 more source
Molecular Oncology, EarlyView.Cytarabine is a key therapy for acute myeloid leukaemia (AML), but its efficacy is limited by the dNTPase SAMHD1, which hydrolyses its active metabolite. Screening nucleotide biosynthesis inhibitors revealed that IMPDH inhibitors selectively sensitise SAMHD1‐proficient AML cells to cytarabine.
Miriam Yagüe‐Capilla +9 more
wiley +1 more source
Advances in biotechnology: Genomics and genome editing
The EuroBiotech Journal, 2017 Abstract Genomics, the study of genes, their functions and related techniques has become a crucial science for developing understanding of life processes and how they evolve. Since the advent of the human genome project, huge strides have been made in developing understanding of DNA and RNA sequence information and how it can be put ...
Gartland, Jill S. +4 more
openaire +3 more sources
Crop Biotechnology: Genetic Modification and Genome Editing
, 2018 Plant molecular biology came to the fore in the early 1980s and there has been tremendous growth in the subject since then. The study of plant genes and genomes, coupled with the development of techniques for the incorporation of novel or modified genes ...
Halford, N. G., Nigel G Halford
core +1 more source
Rapid genome editing by CRISPR-Cas9-POLD3 fusion
, 2021 Precision CRISPR gene editing relies on the cellular homology-directed DNA repair (HDR) to introduce custom DNA sequences to target sites. The HDR editing efficiency varies between cell types and genomic sites, and the sources of this variation are ...
Eero Tolo +42 more
core +1 more source