Results 51 to 60 of about 1,533,500 (299)

Comprehensive assessment of miniature CRISPR-Cas12f nucleases for gene disruption

Nature Communications, 2022
CRISPR-Cas12f nucleases can be effectively packaged into AAVs for gene therapy, but a systematic evaluation of editing outcomes is lacking. Here the authors perform a comprehensive assessment of 4 Cas12f proteins and compare to Cas9 and two Cas12a ...
Changchang Xin, Jianhang Yin, Shaopeng Yuan, Liqiong Ou, Mengzhu Liu, Weiwei Zhang, Jiazhi Hu   +6 more
doaj   +1 more source

From mice to humans—divergent strategies for intestinal homeostasis and regeneration

FEBS Letters, EarlyView.
Recent advances such as organoid genome editing, xenotransplantation, imaging, and whole‐genome sequencing have enabled direct studies of human intestinal stem cells (ISCs). These studies reveal species‐specific features, including slower ISC proliferation, distinct injury responses, slower somatic mutation accumulation in humans, and an inverse ...
Keiko Ishikawa, Shinya Sugimoto, Toshiro Sato   +2 more
wiley   +1 more source

PtWAVE: a high-sensitive deconvolution software of sequencing trace for the detection of large indels in genome editing

BMC Bioinformatics
Background Tracking of Insertions and DEletions (TIDE) analysis, which computationally deconvolves capillary sequencing data derived from the DNA of bulk or clonal cell populations to estimate the efficiency of targeted mutagenesis by programmable ...
Kazuki Nakamae, Saya Ide, Nagaki Ohnuki, Yoshiko Nakagawa, Keisuke Okuhara, Hidemasa Bono   +5 more
doaj   +1 more source

Design and analysis strategies for robust microbiome ageing research

FEBS Letters, EarlyView.
The gut microbiome changes with age and associates with age‐related morbidity and mortality, establishing it as a potential biomarker and intervention target for ageing. Realising this potential requires methodological rigour, yet distinguishing biological signals from methodological artefacts remains challenging across cohorts. This review provides an
Mark Olenik, İsmail Güderer, Yi Wang, Tayyaba Alvi, Prasoon Pandey, Handan Melike Dönertaş   +5 more
wiley   +1 more source

Methodological landscape in the field of integration site identification of retroviruses and retroviral vectors

Frontiers in Bioengineering and Biotechnology
Detailed mapping of viral vector integration sites, including retroviral and particularly lentiviral vectors, is critical for assessing their safety in preclinical and clinical studies.
Konstantin Kochergin-Nikitsky, Alexander Lavrov, Svetlana Smirnikhina   +2 more
doaj   +1 more source

Investigating transcription factor dynamics in health and disease using FRAP

FEBS Letters, EarlyView.
FRAP analysis of GFP‐tagged transcription factors reveals how molecular mobility and target engagement change in response to drug treatment. By combining live‐cell imaging, quantitative model fitting, and statistical analysis, this approach uncovers transcription factor dynamics linked to disease mechanisms, providing a powerful framework for ...
Kannan Govindaraj, Carolina Vazquez Garzon, Jose Joaquin Velasco, Janine N. Post   +3 more
wiley   +1 more source

Double-strand break-free and transgene-free genome editing in the microalga Nannochloropsis oceanica using removable vectors containing the CRISPR base editing system

Scientific Reports
The accumulation of lipids by algae makes them attractive for carbon-neutral fuel production; however, the industrial-scale production of algal lipids has yet to be achieved.
Keishi Moroi, Takashi Yamamoto, Tomokazu Kurita   +2 more
doaj   +1 more source

Extracellular vesicle-based delivery to airway basal cells for durable gene therapy in cystic fibrosis

Frontiers in Bioengineering and Biotechnology
Airway basal cells are an appealing target for durable genetic therapy in cystic fibrosis because they are long-lived stem/progenitor cells that maintain and regenerate the pseudostratified airway epithelium.
Anastasiia Ivleva, Anna Demchenko, Svetlana Smirnikhina   +2 more
doaj   +1 more source

Differentially activated B cells develop regulatory phenotype and show varying immunosuppressive features: a comparative study

Frontiers in Immunology, 2023
Regulatory B lymphocytes (Bregs) are B cells with well-pronounced immunosuppressive properties, allowing them to suppress the activity of effector cells.
Elina A. Zheremyan, Alina S. Ustiugova, Aksinya N. Uvarova, Nina M. Karamushka, Ekaterina M. Stasevich, Violetta S. Gogoleva, Apollinariya V. Bogolyubova, Nikita A. Mitkin, Dmitry V. Kuprash, Dmitry V. Kuprash, Kirill V. Korneev, Kirill V. Korneev   +11 more
doaj   +1 more source

Nutrient/TOR signaling controls adipose mitochondrial transcription factor A (TFAM) to regulate organismal growth in Drosophila

FEBS Letters, EarlyView.
Animals must match their growth rate to available nutrients. We show that in Drosophila larvae, the nutrient‐sensing TOR kinase controls growth by regulating levels of TFAM, a key regulator of mitochondrial function, in the adipose tissue. When nutrients are abundant, high TOR activity suppresses TFAM, lowering mitochondrial bioenergetic activity and ...
Shrivani Sriskanthadevan‐Pirahas, Shahoon Khan, Abhishek Sharma, Byoungchun Lee, Savraj S. Grewal   +4 more
wiley   +1 more source