Results 341 to 350 of about 3,929,879 (390)
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Phage genome engineering with retrons
Nature BiotechnologyIlya Osterman, R. Sorek
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Nature Biotechnology, 2009
For more than 50 years, those engineering genetic material have pursued increasingly challenging targets. During that time, the tools and resources available to the genetic engineer have grown to encompass new extremes of both scale and precision, opening up new opportunities in genome engineering.
Peter A, Carr, George M, Church
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For more than 50 years, those engineering genetic material have pursued increasingly challenging targets. During that time, the tools and resources available to the genetic engineer have grown to encompass new extremes of both scale and precision, opening up new opportunities in genome engineering.
Peter A, Carr, George M, Church
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Directed genome engineering for genome optimization
The International Journal of Developmental Biology, 2013The ability to develop nucleases with tailor-made activities for targeted DNA double-strand break induction at will at any desired position in the genome has been a major breakthrough to make targeted genome optimization feasible in plants. The development of site specific nucleases for precise genome modification has expanded the repertoire of tools ...
Kathleen, D'Halluin, Rene, Ruiter
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Porcine genome engineering for xenotransplantation
Advanced Drug Delivery Reviews, 2021The extreme shortage of human donor organs for treatment of patients with end-stage organ failures is well known. Xenotransplantation, which might provide unlimited organ supply, is a most promising strategy to solve this problem. Domestic pigs are regarded as ideal organ-source animals owing to similarity in anatomy, physiology and organ size to ...
Dong, Niu +10 more
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Actinomycetes genome engineering approaches
Antonie van Leeuwenhoek, 2012This review provides an overview of new technologies for DNA manipulations in actinomycetes exploiting recombinogenic engineering (Flp-FRT, Cre-loxP, Dre-rox, Tn5, GusA and I-SceI systems). We will describe some new vectors recently developed for engineering of complex phenotypes in actinomycetes.
Theresa, Siegl, Andriy, Luzhetskyy
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Engineering genomes in multiplex
Current Opinion in Biotechnology, 2011Efficiently engineering robust complex traits is a key challenge facing metabolic engineering efforts to synthesize valuable products in vivo. Recent advances in genome engineering confront this barrier and significantly enhance the ability to map functional changes targeted throughout the genome and combinatorially optimize complex (multigenic) traits
Lauren B A, Woodruff, Ryan T, Gill
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Genome engineering: the next genomic revolution
Nature Methods, 2014A decade of advances in genome engineering technologies has enabled the editing of genome sequences much like one edits computer code; many more applications for precisely manipulating genome structure and function are on the horizon.
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IEEE Engineering in Medicine and Biology Magazine, 1999
This work addresses two principles that will be integral to the post-genomic or proteomic era (i.e., after sequencing). The first is that any analysis of data from or related to the Human Genome Project will need to be designed with high-throughput in mind.
M.V. Boland, R.F. Murphy
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This work addresses two principles that will be integral to the post-genomic or proteomic era (i.e., after sequencing). The first is that any analysis of data from or related to the Human Genome Project will need to be designed with high-throughput in mind.
M.V. Boland, R.F. Murphy
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Genome Engineering Using TALENs
2018Genome engineering involves methods of genetic modification of cells at predefined genomic sites. Here, we used transcription activator-like effector nucleases (TALENs) for the site-directed mutagenesis in barley. Target gene-specific TALEN-encoding expression units were designed and delivered to totipotent cells of either cultivated embryogenic pollen
Goetz, Hensel, Jochen, Kumlehn
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Engineering three-dimensional genome folding
Nature Genetics, 2021Animal genomes are partitioned and folded at various scales that contribute distinctly to nuclear processes. While structural features have been disrupted either globally or at select loci in loss-of-function studies, gain-of-function studies that probe the role of genome architecture have lagged behind.
Di Zhang, Jessica Lam, Gerd A. Blobel
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