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Multiplexed Genome Engineering with Cas12a
2021Genome engineering technologies based on CRISPR-Cas systems are fueling efforts to study genotype-phenotype relationships in a high-throughput and multiplexed fashion. While many genome engineering technologies exist and provide a means to efficiently manipulate one or a few genes in a singular context-knockout, inhibition, or activation in a ...
Niels R, Weisbach +2 more
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Evolutionary engineering by genome shuffling
Applied Microbiology and Biotechnology, 2014An upsurge in the bioeconomy drives the need for engineering microorganisms with increasingly complex phenotypes. Gains in productivity of industrial microbes depend on the development of improved strains. Classical strain improvement programmes for the generation, screening and isolation of such mutant strains have existed for several decades.
Damien, Biot-Pelletier +1 more
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Microbial Base Editing: A Powerful Emerging Technology for Microbial Genome Engineering.
Trends in Biotechnology, 2020Genome engineering is crucial for answering fundamental questions about, and exploring practical applications of, microorganisms. Various microbial genome-engineering tools, including CRISPR/Cas-enhanced homologous recombination (HR), have been developed,
Yu Wang +4 more
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Programmable genome engineering
Science, 2019Synthetic Biology The model bacterium Escherichia coli has a single circular chromosome. Wang et al. created a method to fragment the E. coli genome into independent chromosomes that can be modified, rearranged, and recombined. The efficient fission of the unmodified E. coli genome into two defined, stable pairs of synthetic chromosomes provides common
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1998
• DNA transformation has removed most of the barriers that limit the introduction of specific genes from one organism to another. • The use of Agrobacteria and modified Ti plasmids to transform dicotyledons, and the use of microinjection to transfect the nuclei of animal cells, are being superseded by particle-bombardment biolistic procedures ...
Rudi Appels +3 more
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• DNA transformation has removed most of the barriers that limit the introduction of specific genes from one organism to another. • The use of Agrobacteria and modified Ti plasmids to transform dicotyledons, and the use of microinjection to transfect the nuclei of animal cells, are being superseded by particle-bombardment biolistic procedures ...
Rudi Appels +3 more
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Genomics and Genetic Engineering
2011The hurricane growth of genomics and genetic engineering poses challenging ethical questions pertaining to the technological application in human life. Many secular and religious bioethicists observe that the new proposals of genetic engineering are described as “playing God.” The metaphor has evoked both optimistic and pessimistic perspectives among ...
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Genome Engineering with Custom Recombinases
2014Site-specific recombinases are valuable tools for myriad basic research and genome engineering applications. In particular, hybrid recombinases consisting of catalytic domains from the resolvase/invertase family of serine recombinases fused to Cys2-His2 zinc-finger or TAL effector DNA-binding domains are capable of introducing targeted modifications ...
Thomas, Gaj, Carlos F, Barbas
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Genome Engineering in Human Cells
2014Genome editing in human cells is of great value in research, medicine, and biotechnology. Programmable nucleases including zinc-finger nucleases, transcription activator-like effector nucleases, and RNA-guided engineered nucleases recognize a specific target sequence and make a double-strand break at that site, which can result in gene disruption, gene
Minjung, Song +3 more
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Conjugative Assembly Genome Engineering (CAGE)
2019Conjugative assembly genome engineering (CAGE) enables the transfer of large chromosomal regions from a donor to a recipient. Specific regions of the donor chromosome can be introduced in the recipient genome by the directed insertion of an origin of transfer and two selection cassettes.
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