Results 51 to 60 of about 155,866 (241)

A subset of MMR‐proficient colon cancers responds to neoadjuvant immunotherapy

Molecular Oncology, EarlyView.
Tan et al. reveal that a distinct subset of early‐stage pMMR colon cancers can respond to neoadjuvant immunotherapy. In the NICHE‐2 trial, responders (26%) were characterized by chromosomal instability, TP53 mutations, and proliferative cell‐cycle programs, whereas nonresponders showed metabolic and stromal reprogramming with TGF‐β‐driven ...
Eleonora Piumatti, Giovanni Germano, Pietro Paolo Vitiello, Alberto Bardelli   +3 more
wiley   +1 more source

Protocol for high-throughput screening of SIRT7 inhibitors using fluorescent peptide technology

STAR Protocols
Summary: Fluorescent peptides combine the polypeptides with fluorescent groups organically to facilitate the measurement of enzyme activity. Here, we present a protocol to evaluate Sirtuin 7 (SIRT7) enzymatic activity by analyzing changes in luminescent ...
Tian-Shu Kang, Xiaopeng Lu, Jinke Gu, Yuan Tian, Wei-Guo Zhu   +4 more
doaj   +1 more source

A Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells

Cell Reports, 2019
Summary: The recent availability of somatic haploid cell lines has provided a unique tool for genetic studies in mammals. However, the percentage of haploid cells rapidly decreases in these cell lines, which we recently showed is due to their overgrowth ...
Teresa Olbrich, Maria Vega-Sendino, Matilde Murga, Guillermo de Carcer, Marcos Malumbres, Sagrario Ortega, Sergio Ruiz, Oscar Fernandez-Capetillo   +7 more
doaj   +1 more source

Decoding protein methylation function with thermal stability analysis

Nature Communications, 2023
Protein methylation is an important modification beyond epigenetics. However, systems analyses of protein methylation lag behind compared to other modifications.
Cristina Sayago, Jana Sánchez-Wandelmer, Fernando García, Begoña Hurtado, Vanesa Lafarga, Patricia Prieto, Eduardo Zarzuela, Pilar Ximénez-Embún, Sagrario Ortega, Diego Megías, Oscar Fernández-Capetillo, Marcos Malumbres, Javier Munoz   +12 more
doaj   +1 more source

MicroRNAs, Genomic Instability and Cancer [PDF]

International Journal of Molecular Sciences, 2014
MicroRNAs (miRNAs) are small non-coding RNA transcripts approximately 20 nucleotides in length that regulate expression of protein-coding genes via complementary binding mechanisms. The last decade has seen an exponential increase of publications on miRNAs, ranging from every aspect of basic cancer biology to diagnostic and therapeutic explorations ...
Vincent, Kimberly, Pichler, Martin, Lee, Gyeong-Won, Ling, Hui   +3 more
openaire   +3 more sources

PARP inhibition and pharmacological ascorbate demonstrate synergy in castration‐resistant prostate cancer

Molecular Oncology, EarlyView.
Pharmacologic ascorbate (vitamin C) increases ROS, disrupts cellular metabolism, and induces DNA damage in CRPC cells. These effects sensitize tumors to PARP inhibition, producing synergistic growth suppression with olaparib in vitro and significantly delayed tumor progression in vivo. Pyruvate rescue confirms ROS‐dependent activity.
Nicolas Gordon, Peter T. Gallagher, Orly I. Richter, Neermala Poudel Neupane, Amy C. Mandigo, Jennifer J. McCann, Emanuela Dylgjeri, Irina Vasilevskaya, Christopher McNair, Channing J. Paller, Wm. Kevin Kelly, Karen E. Knudsen, Matthew J. Schiewer, Ayesha A. Shafi   +13 more
wiley   +1 more source

H1N76/77 deamidation facilitates chromatin remodelling and genome stability during DNA damage repair

Clinical and Translational Medicine
Chromatin relaxation is a permissiven progress for DNA repair through enabling repair factors to access the damaged DNA. Linker histone H1 is important in maintaining chromatin compaction under physiological state.
Tingting Feng, Mengyan Li, Chenmiao Hu, Yuan Tian, Wei‐Guo Zhu   +4 more
doaj   +1 more source

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

WDFY2 promotes MRN complex formation required for homologous recombination-mediated DNA repair

Cell Reports
Summary: The MRE11-RAD50-NBS1 (MRN) complex is fundamental for detecting and repairing DNA double-strand breaks (DSBs), thereby safeguarding genome integrity.
Ya-Fei Lu, Huangqi Tang, Longjiang Di, Yerkezhan Yerkinkazhina, Jiajie Su, Ming Tang, Jinqin Qian, Rongsheng Zeng, Yuxin Shu, Xingkai He, Hui Yang, Jun Zhang, Yuan Tian, Qian Zhu, Luyao Zhang, Yongqing Wang, Dongchun Ni, Xin-Hai Pei, Ying Zhao, Chung-Hang Leung, Xiaopeng Lu, Wei-Guo Zhu   +21 more
doaj   +1 more source

Heterozygotic Brca1 mutation initiates mouse genome instability at embryonic stage

Oncogenesis, 2022
BRCA1 mutation is the genetic predisposition in causing genome instability towards cancer. BRCA1 mutation is predominantly germline inherited at the fertilization. However, when the inherited mutation initiates genome instability in the mutation carriers
Xiaobing Wu, Maoni Guo, Jian Cui, Haoyang Cai, San Ming Wang   +4 more
doaj   +1 more source