Results 211 to 220 of about 4,549,742 (256)
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2018
Strategies for sequencing fungal genomes on next-generation sequencing (NGS) platforms depend on the characteristics of the genome of the targeted species, quantity and quality of the genomic DNA, and cost considerations. Massively parallel sequencing with sequencing by synthesis (SBS) approach by Illumina produces terabases of short read sequences (i ...
Yuko, Yoshinaga +3 more
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Strategies for sequencing fungal genomes on next-generation sequencing (NGS) platforms depend on the characteristics of the genome of the targeted species, quantity and quality of the genomic DNA, and cost considerations. Massively parallel sequencing with sequencing by synthesis (SBS) approach by Illumina produces terabases of short read sequences (i ...
Yuko, Yoshinaga +3 more
openaire +2 more sources
2016
Genome sequencing is an important step toward correlating genotypes with phenotypic characters. Sequencing technologies are important in many fields in the life sciences, including functional genomics, transcriptomics, oncology, evolutionary biology, forensic sciences, and many more. The era of sequencing has been divided into three generations.
Mansi, Verma +2 more
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Genome sequencing is an important step toward correlating genotypes with phenotypic characters. Sequencing technologies are important in many fields in the life sciences, including functional genomics, transcriptomics, oncology, evolutionary biology, forensic sciences, and many more. The era of sequencing has been divided into three generations.
Mansi, Verma +2 more
openaire +2 more sources
Science, 1997
The human genome project is at the halfway point. The genomes of 11 microbes, Escherichia coli , and yeast are finished, yet the human genome is only 2 percent finished. The scale-up to finish by 2005 presents a significant challenge.
Leroy Hood +2 more
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The human genome project is at the halfway point. The genomes of 11 microbes, Escherichia coli , and yeast are finished, yet the human genome is only 2 percent finished. The scale-up to finish by 2005 presents a significant challenge.
Leroy Hood +2 more
openaire +6 more sources
Genome Sequencing and Genomics
2014This chapter explores issues related to Legionella genomics. A critical breakthrough in this area recently occurred with the determination of the genome sequences of three clinical, serogroup 1 isolates of Legionella pneumophila subsp. pneumophila. The three strains are L. pneumophila Paris, Lens and Philadelphia-1.
Joseph P. Vogel +3 more
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Genome Sequencing and Assembly
2011Decoding the genome sequence is becoming a fundamental tool for molecular, genetic, and genomic studies. This chapter reviews the history of DNA sequencing and technical principles of different sequencing platforms, and compares the strengths and weaknesses of different techniques for high-throughput genome sequencing applications are compared. It also
Manfred Grabherr +2 more
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2009
For over 30 yr, the Sanger method has been the standard for DNA sequencing. Instruments have been developed and improved over time to increase throughput, but they always relied on the same technology. Today, we are facing a revolution in DNA sequencing with many drastically different platforms that have become or will soon become available on the ...
Hervé Tettelin, T. Feldblyum
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For over 30 yr, the Sanger method has been the standard for DNA sequencing. Instruments have been developed and improved over time to increase throughput, but they always relied on the same technology. Today, we are facing a revolution in DNA sequencing with many drastically different platforms that have become or will soon become available on the ...
Hervé Tettelin, T. Feldblyum
openaire +2 more sources
Science, 1996
I have learned that the U.S. National Institutes of Health (NIH) is to support a program to sequence the genome of Microbacterium tuberculosis . While this information is desperately needed to advance studies that could provide essential clues to the pathogenesis and effective treatment of tuberculosis, I am dismayed and disappointed by the decision ...
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I have learned that the U.S. National Institutes of Health (NIH) is to support a program to sequence the genome of Microbacterium tuberculosis . While this information is desperately needed to advance studies that could provide essential clues to the pathogenesis and effective treatment of tuberculosis, I am dismayed and disappointed by the decision ...
openaire +3 more sources
2014
The current Lotus japonicus reference genome sequence is based on a hybrid assembly of Sanger TAC/BAC, Sanger shotgun and Illumina shotgun sequencing data generated from the Miyakojima-MG20 accession. It covers nearly all expressed L. japonicus genes and has been annotated mainly based on transcriptional evidence.
Sato, Shusei, Andersen, Stig Uggerhøj
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The current Lotus japonicus reference genome sequence is based on a hybrid assembly of Sanger TAC/BAC, Sanger shotgun and Illumina shotgun sequencing data generated from the Miyakojima-MG20 accession. It covers nearly all expressed L. japonicus genes and has been annotated mainly based on transcriptional evidence.
Sato, Shusei, Andersen, Stig Uggerhøj
openaire +1 more source
Current Opinion in Plant Biology, 2004
Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads.
Pablo D. Rabinowicz +3 more
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Sequencing of complex genomes can be accomplished by enriching shotgun libraries for genes. In maize, gene-enrichment by copy-number normalization (high C(0)t) and methylation filtration (MF) have been used to generate up to two-fold coverage of the gene-space with less than 1 million sequencing reads.
Pablo D. Rabinowicz +3 more
openaire +3 more sources
Genome sequencing networks [PDF]
Nature Reviews Genetics, 2001 Genome sequencing projects have been undertaken in one of three ways: in a purpose-built and professionally staffed genome centre, by a small number of traditional research laboratories or by an extensive network of traditional research laboratories that are linked by the Internet.
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