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Genomic Libraries: I. Construction and Screening of Fosmid Genomic Libraries
2011Large insert genome libraries have been a core resource required to sequence genomes, analyze haplotypes, and aid gene discovery. While next generation sequencing technologies are revolutionizing the field of genomics, traditional genome libraries will still be required for accurate genome assembly.
Lucy Matthews +5 more
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OBTAINING GENOMIC DNA FOR CONSTRUCTION OF GENOMIC LIBRARY
Acta Horticulturae, 2010The isolation of pure genomic DNA from the white and red dragon fruits, Hylocereus undatus and Hylocereus polyrhizus (Cactaceae) for the construction of a genomic library is described. These vine cacti originate from northern South America, Central America and Mexico, and are cultivated as new exotic fruit crops in SE Asia.
S. Chandran, Z. X. Loo, L. K. P. Daniel
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Indexing genomic sequence libraries
Information Processing & Management, 2005This paper describes an extensible, open-source (GPL) data repository and retrieval system that supports fast, efficient, keyword based retrieval of genomic sequences from multiple libraries with retrieved sequences post-processed by FASTA, Smith-Waterman and other analysis software. This application is implemented for Linux and is written in Mumps, C,
Matthew J. Lockner, Kevin C. O'Kane
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Current Protocols in Molecular Biology, 1987
AbstractGenomic DNA libraries are almost always screened by hybridization using a radioactive nucleic acid probe. Since this approach is essentially independent of a particular vector or type of target DNA, the main problem faced when considering creation of a genomic DNA library is simply generating a large enough number of recombinant DNA clones. The
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AbstractGenomic DNA libraries are almost always screened by hybridization using a radioactive nucleic acid probe. Since this approach is essentially independent of a particular vector or type of target DNA, the main problem faced when considering creation of a genomic DNA library is simply generating a large enough number of recombinant DNA clones. The
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Rapid Expression of Functional Genomic Libraries
Journal of Proteome Research, 2006Genomic-scale analysis of protein function is currently limited by the ability to rapidly express the enormous diversity of protein targets in their active form. We describe a method to construct transcriptionally active expression templates (ETs) in parallel using a single PCR step wherein the overlap-extension reaction for addition of transcription ...
Kim A. Woodrow +2 more
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Production of a Genomic
AbstractThe describes the strategy of producing a genomic DNA library. A number of small‐scale ligations are performed using a set amount of vector and varying amounts of insert. Test ligations are transformed into bacteria (plasmid vectors) or packaged and plated on host bacteria (λ and cosmid vectors). The number of clones in the different ligations
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Genetic engineering: Lassoing genomic libraries [PDF]
Nature Biomedical Engineering, 2017 Long single-stranded DNA probes enable the capture and multiplexed cloning of DNA at the megabase scale.
Nathan B. Lubock, Sriram Kosuri
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1998
1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
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1. Theory and Techniques of Library Construction Genomic DNA libraries are a collection of DNA fragments that together represent the entire (or nearly entire) genome of the mdividual from which the DNA was derived. These fragments are contained within self-rephcatmg vectors that enable them to be mamtamed and propagated within the cells of ...
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Integrating Sinorhizobium meliloti Genomics: An Expression Library of the Genome
2008through Sustainable Agriculture. © Springer Science + Business Media B.V. 2008 F. D. Dakora et al. (eds.), Biological Nitrogen Fixation: Towards Poverty Alleviation 293 To investigate gene function, we developed a robust transcription fusion reporter vector to measure gene expression in bacteria.
Alison Cowie +6 more
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Methods for generating plant genomic libraries [PDF]
, 1994 The genome of plants is remarkably complex and a particular DNA fragment of interest comprises only a small fraction of the whole genome. Therefore, construction of a useful and representative recombinant genomic library is dependent on the generation of a large population of clones.
Jay M. Short +2 more
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