Results 71 to 80 of about 178,565 (255)

Efficient cloning system for construction of gene silencing vectors in Aspergillus niger [PDF]

, 2008
An approach based on Gateway recombination technology to efficiently construct silencing vectors was developed for use in the biotechnologically important fungus Aspergillus niger. The transcription activator of xylanolytic and cellulolytic genes XlnR of
Graaff, L.H., de, Oliveira, J.M., Qin Ling, Veen, D., van der   +3 more
core   +2 more sources

Detection of circulating tumor DNA in colorectal cancer patients using a methylation‐specific droplet digital PCR multiplex

Molecular Oncology, EarlyView.
We developed a cost‐effective methylation‐specific droplet digital PCR multiplex assay containing tissue‐conserved and tumor‐specific methylation markers. The assay can detect circulating tumor DNA with high accuracy in patients with localized and metastatic colorectal cancer.
Luisa Matos do Canto, Louise Raunkilde, Jan Lindebjerg, Mads Malik Aagaard, Christina Therkildsen, Jakob Kleif, Lars Henrik Jensen, Torben Frøstrup Hansen, Rikke Fredslund Andersen   +8 more
wiley   +1 more source

Crystal structure of yeast DNA polymerase ε catalytic domain. [PDF]

PLoS ONE, 2014
DNA polymerase ε (Polε) is a multi-subunit polymerase that contributes to genomic stability via its roles in leading strand replication and the repair of damaged DNA.
Rinku Jain, Kanagalaghatta R Rajashankar, Angeliki Buku, Robert E Johnson, Louise Prakash, Satya Prakash, Aneel K Aggarwal   +6 more
doaj   +1 more source

Is HIV-1 RNA dimerization a prerequisite for packaging? Yes, no, probably? [PDF]

, 2004
During virus assembly, all retroviruses specifically encapsidate two copies of full-length viral genomic RNA in the form of a non-covalently linked RNA dimer.
Liang, Chen, Russell, Rodney S, Wainberg, Mark A   +2 more
core   +3 more sources

Effective therapeutic targeting of CTNNB1‐mutant hepatoblastoma with WNTinib

Molecular Oncology, EarlyView.
WNTinib, a Wnt/CTNNB1 inhibitor, was tested in hepatoblastoma (HB) experimental models. It delayed tumor growth and improved survival in CTNNB1‐mutant in vivo models. In organoids, WNTinib outperformed cisplatin and showed enhanced efficacy in combination therapy, supporting its potential as a targeted treatment for CTNNB1‐mutated HB.
Ugne Balaseviciute, Júlia Huguet‐Pradell, Jordi Abril‐Fornaguera, Albert Gris‐Oliver, Alex Rialdi, Elisa Fernández‐Martínez, Carla Montironi, Vanessa Del Pozo, Peter Houghton, Laura Zanatto, Agavni Mesropian, Ieva Keraite, Swan Thung, Carolina Armengol, Pau Sancho‐Bru, Ernesto Guccione, Roser Pinyol, Josep M. Llovet   +17 more
wiley   +1 more source

Ribonucleolytic resection is required for repair of strand displaced nonhomologous end-joining intermediates [PDF]

, 2013
Nonhomologous end-joining (NHEJ) pathways repair DNA double-strand breaks (DSBs) in eukaryotes and many prokaryotes, although it is not reported to operate in the third domain of life, archaea. Here, we describe a complete NHEJ complex, consisting of DNA
Bartlett, Edward, Brissett, Nigel C, Doherty, Aidan J   +2 more
core   +1 more source

Plecstatin inhibits hepatocellular carcinoma tumorigenesis and invasion through cytolinker plectin

Molecular Oncology, EarlyView.
The ruthenium‐based metallodrug plecstatin exerts its anticancer effect in hepatocellular carcinoma (HCC) primarily through selective targeting of plectin. By disrupting plectin‐mediated cytoskeletal organization, plecstatin inhibits anchorage‐dependent growth, cell polarization, and tumor cell dissemination.
Zuzana Outla, Jan Kosla, Magdalena Prechova, Lukas Frick, Patricia Bortel, Yasmin Borutzki, Andrea Bileck, Christopher Gerner, Samuel M. Meier‐Menches, Selma Osmanagic‐Myers, Martin Gregor   +10 more
wiley   +1 more source

Capturing the essence of folding and functions of biomolecules using Coarse-Grained Models

, 2011
The distances over which biological molecules and their complexes can function range from a few nanometres, in the case of folded structures, to millimetres, for example during chromosome organization. Describing phenomena that cover such diverse length,
A Dhar, A Elcock, A Elcock, A Grosberg, A Grosberg, A Minton, A Pisliakov, A Podtelezhnikov, A Rychkova, A Savelyev, A Vologodskii, AA Podtelezhnikov, B Messer, BJ Alder, C Bustamante, C Clementi, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, C Hyeon, D Thirumalai, D Thirumalai, D Thirumalai, D Thirumalai, D Thirumalai, E Koculi, E Lieberman-Aiden, E O'Brien, E Shakhnovich, EP O'Brien, F Valle, G Lorimer, G Papoian, G Ziv, H Liu, H Ma, HX Zhou, I Tinoco Jr., J Chen, J Harms, J Karanicolas, J Straub, JD Honeycutt, JM Fernandez, JN Onuchic, KA Dill, M Levitt, M Mickler, M Strajbl, M Takano, ME Fisher, MJ Todd, MS Cheung, N Hirokawa, N Koga, N Toan, OK Dudko, P Weinkam, PB Moore, PC Whitford, Q Du, R Russell, R Tehver, R Tehver, RD Vale, RD Vale, RI Dima, RW Pastor, S Jun, S Jun, S Jun, S Kamerlin, S Kirkpatrick, S Moghaddam, SB Smith, SJ Chen, SJ Marrink, T Cloutier, T Cremer, T Kirkpatrick, T Veitshans, V Lubchenko, Z Liu, Z Xu   +89 more
core   +1 more source

Phenotypic and genotypic characterization of single circulating tumor cells in the follow‐up of high‐grade serous ovarian cancer

Molecular Oncology, EarlyView.
Single circulating tumor cells (sCTCs) from high‐grade serous ovarian cancer patients were enriched, imaged, and genomically profiled using WGA and NGS at different time points during treatment. sCTCs revealed enrichment of alterations in Chromosomes 2, 7, and 12 as well as persistent or emerging oncogenic CNAs, supporting sCTC identity.
Carolin Salmon, Rui P. L. Neves, Nikolas H. Stoecklein, Sven‐Thorsten Liffers, Jens Siveke, Jan D. Kuhlmann, Pauline Wimberger, Paul Buderath, Rainer Kimmig, Sabine Kasimir‐Bauer   +9 more
wiley   +1 more source

Correlation of the differential expression of PIK3R1 and its spliced variant, p55α, in pan‐cancer

Molecular Oncology, EarlyView.
PIK3R1 undergoes alternative splicing to generate the isoforms, p85α and p55α. By combining large patient datasets with laboratory experiments, we show that PIK3R1 spliced variants shape cancer behavior. While tumors lose the protective p85α isoform, p55α is overexpressed, changes linked to poorer survival and more pronounced in African American ...
Ishita Gupta, Yang Song, Madeleine Ndahayo, Anirudh Saxena, Theresa Guo, Dylan Z. Kelley, Jessica Gore, Andrew Hennigan, Alexa Anderson, John C. Papadimitriou, Daria A. Gaykalova   +10 more
wiley   +1 more source