Results 91 to 100 of about 16,273 (193)

Testing reference genes for transcript profiling in Uromyces appendiculatus during urediospore infection of common bean.

PLoS ONE, 2020
Uromyces appendiculatus is a major pathogen on common bean. Like other rust fungi, it uses effectors to influence its host plant. Effectors are assumed to possess characteristic expression profiles, reflecting their activity during the infection process.
Tobias I Link
doaj   +1 more source

Altered expression of neuroplasticity-related genes in the brain of depressed suicides [PDF]

, 2015
Background: Expression of the neuronal membrane glycoprotein M6a (GPM6A), the proteolipid protein (PLP/DM20) family member, is downregulated in the hippocampus of chronically stressed animals.
Alvarez Juliá, Anabel, Frasch, Alberto Carlos C., Fuchsova, Beata, Pandey, G. N., Rizavi, H. S.   +4 more
core   +1 more source

Identification of stable reference genes for quantitative PCR in koalas [PDF]

, 2018
To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation.
A Kappel, A Radonic, B Artegiani, CA Waugh, CL Andersen, D Kim, DG Ginzinger, F Almeida-Oliveira, F Bartz, G Bamias, GS Meers, GS Simmons, IE Maher, IE Maher, J Vandesompele, J Woinarski, J Yperman, JJ Hanger, K Ahn, KC Thomas, KM Morris, M Kubista, M Pertea, MA Valasek, MW Pfaffl, N Silver, OT Ong, P Brym, PD Lee, PJ Canfield, QL Zhang, R Tarlinton, R Tarlinton, RK Das, S Bages, SA Bustin, SA Bustin, T Burmeister, T Nolan, V Gonzalez-Astudillo, Y Zhang   +40 more
core   +4 more sources

Reference gene stability of a synanthropic fly, Chrysomya megacephala

Parasites & Vectors, 2015
Background Stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qPCR).
Xiaoyun Wang, Mei Xiong, Jialu Wang, Chaoliang Lei, Fen Zhu   +4 more
doaj   +1 more source

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Frontiers in Plant Science, 2017
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang   +5 more
doaj   +1 more source

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]

, 2012
Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Kaiser, Sebastian, Meyer, Swanhild U., Pfaffl, Michael W., Thirion, Christian, Wagner, Carola   +4 more
core   +1 more source

Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR [PDF]

, 2008
Flatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using ...
Infante, C.
core   +1 more source

Endogenous reference genes for gene expression studies on bicuspid aortic valve associated aortopathy in humans

, 2016
Bicuspid aortic valve (BAV) disease is the most common congenital cardiac abnormality and predisposes patients to life-threatening aortic complications including aortic aneurysm.
Cagampang, Felino, Harrison, Oliver, Moorjani, Narain, Ohri, Sunil, Torrens, Christopher   +4 more
core   +3 more sources

Tracking the best reference genes for RT-qPCR data normalization in filamentous fungi [PDF]

, 2015
Background: A critical step in the RT-qPCR workflow for studying gene expression is data normalization, one of the strategies being the use of reference genes.
Agustina Llanos, Jean François, Jean-Luc Parrou   +2 more
core   +3 more sources

Selection of Reference Genes for Quantitative Gene Expression in Porcine Mesenchymal Stem Cells Derived from Various Sources along with Differentiation into Multilineages

Stem Cells International, 2015
The identification of stable reference genes is a prerequisite for ensuring accurate validation of gene expression, yet too little is known about stable reference genes of porcine MSCs.
Won-Jae Lee, Ryoung-Hoon Jeon, Si-Jung Jang, Ji-Sung Park, Seung-Chan Lee, Raghavendra Baregundi Subbarao, Sung-Lim Lee, Bong-Wook Park, William Allan King, Gyu-Jin Rho   +9 more
doaj   +1 more source