Results 91 to 100 of about 16,155 (172)

Selection of housekeeping genes for gene expression studies in the adult rat submandibular gland under normal, inflamed, atrophic and regenerative states [PDF]

, 2008
Background: Real-time PCR is a reliable tool with which to measure mRNA transcripts, and provides valuable information on gene expression profiles. Endogenous controls such as housekeeping genes are used to normalise mRNA levels between samples for ...
Nicholas Silver, Emanuele Cotroneo, Gordon Proctor, Samira Osailan, Katherine L Paterson, Guy H Carpenter   +5 more
core   +1 more source

Comprehensive profiling of lncRNAs and mRNAs enriched in small extracellular vesicles for early noninvasive detection of colorectal cancer: diagnostic panel assembly and extensive validation

Molecular Oncology, Volume 19, Issue 11, Page 3445-3462, November 2025.
Small extracellular vesicles are a promising source of diagnostic molecules. We conducted a comprehensive study, including transcriptome profiling and RT‐qPCR validation on large cohorts of samples. Diagnostic panels enabling sensitive detection of colorectal cancer and precancerous lesions were established. Some molecules were differentially expressed
Petra Vychytilova‐Faltejskova, Marketa Pavlikova, Lucie Pifkova, Robin Jugas, Tana Machackova, Lenka Radova, Milana Sachlova, Renata Bartosova, Tetiana Samoilenko, Zuzana Feckova, Jana Orlickova, Erika Slamova, Elleni Ponechal Michu, Dagmar Al Tukmachi, Michaela Ruckova, Martina Vodinska, Jan Kotoucek, Tina Catela Ivkovic, Marie Boudna, Lucia Bohovicova, Teodor Stanek, Jana Halamkova, Martin Svoboda, Vladimir Prochazka, Tomas Grolich, Zdenek Kala, Ondrej Slaby   +26 more
wiley   +1 more source

Reference gene stability of a synanthropic fly, Chrysomya megacephala

Parasites & Vectors, 2015
Background Stable reference genes are essential for accurate normalization in gene expression studies with reverse transcription quantitative polymerase chain reaction (qPCR).
Xiaoyun Wang, Mei Xiong, Jialu Wang, Chaoliang Lei, Fen Zhu   +4 more
doaj   +1 more source

Selection and Validation of Appropriate Reference Genes for qRT-PCR Analysis in Isatis indigotica Fort.

Frontiers in Plant Science, 2017
Due to its sensitivity and specificity, real-time quantitative PCR (qRT-PCR) is a popular technique for investigating gene expression levels in plants.
Tao Li, Jing Wang, Miao Lu, Tianyi Zhang, Xinyun Qu, Zhezhi Wang   +5 more
doaj   +1 more source

Profound effect of profiling platform and normalization strategy on detection of differentially expressed microRNAs [PDF]

, 2012
Adequate normalization minimizes the effects of systematic technical variations and is a prerequisite for getting meaningful biological changes. However, there is inconsistency about miRNA normalization performances and recommendations.
Kaiser, Sebastian, Meyer, Swanhild U., Pfaffl, Michael W., Thirion, Christian, Wagner, Carola   +4 more
core   +1 more source

Selection of housekeeping genes for gene expression studies in larvae from flatfish using real-time PCR [PDF]

, 2008
Flatfish metamorphosis involves major physiological and morphological changes. Due to its importance in aquaculture and as a model for developmental studies, some gene expression studies have focused on the understanding of this process using ...
Infante, C.
core   +1 more source

Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows [PDF]

, 2013
peer-reviewedBackground Studies have shown clear differences between dairy breeds in their feed intake and production efficiencies. The duodenum is critical in the coordination of digestion and absorption of nutrients.
Buckley, Frank, Kenny, David A., Keogh, Kate, Waters, Sinead M.   +3 more
core   +1 more source

Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange [PDF]

, 2015
The sheep scab mite, Psoroptes ovis, is a major problem in the beef cattle industry, especially in Belgian Blue (BB) cattle. This breed is naturally more predisposed to psoroptic mange but reasons for this high susceptibility remain unknown.
Chiers, Koen, Claerebout, Edwin, Geldhof, Peter, González Hernández, Ana, Grauwet, Korneel, Grit, Grietje, Sarre, Charlotte, Van Coppernolle, Stefanie, Van Den Broeck, Wim, Van Meulder, Frederik   +9 more
core   +4 more sources

Selection of Reference Genes for Quantitative Gene Expression in Porcine Mesenchymal Stem Cells Derived from Various Sources along with Differentiation into Multilineages

Stem Cells International, 2015
The identification of stable reference genes is a prerequisite for ensuring accurate validation of gene expression, yet too little is known about stable reference genes of porcine MSCs.
Won-Jae Lee, Ryoung-Hoon Jeon, Si-Jung Jang, Ji-Sung Park, Seung-Chan Lee, Raghavendra Baregundi Subbarao, Sung-Lim Lee, Bong-Wook Park, William Allan King, Gyu-Jin Rho   +9 more
doaj   +1 more source

The effect of exogenous glucose infusion on early embryonic development in lactating dairy cows [PDF]

, 2018
peer-reviewedThe objective of this study was to examine the effect of intravenous infusion of glucose on early embryonic development in lactating dairy cows. Nonpregnant, lactating dairy cows (n = 12) were enrolled in the study (276 ± 17 d in milk). On d
Butler, Stephen, Curran, F., Forde, Niamh, Herlihy, Mary M., Kenneally, J., Leane, S., Lonergan, P., Lucy, Matt C., Simintiras, Constantine A., Sturmey, Roger G.   +9 more
core   +2 more sources