Results 101 to 110 of about 16,273 (193)

Comparative immune responses against Psoroptes ovis in two cattle breeds with different susceptibility to mange [PDF]

, 2015
The sheep scab mite, Psoroptes ovis, is a major problem in the beef cattle industry, especially in Belgian Blue (BB) cattle. This breed is naturally more predisposed to psoroptic mange but reasons for this high susceptibility remain unknown.
Chiers, Koen, Claerebout, Edwin, Geldhof, Peter, González Hernández, Ana, Grauwet, Korneel, Grit, Grietje, Sarre, Charlotte, Van Coppernolle, Stefanie, Van Den Broeck, Wim, Van Meulder, Frederik   +9 more
core   +4 more sources

Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors [PDF]

, 2013
BACKGROUND: Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and ...
Astudillo, Aurora, Escudero, Dolores, Gómez, Enrique, Otero, Jesús, Pérez, Silvia, Rodríguez, Aida, Royo, L.J. (Luis), Álvarez, Francisco   +7 more
core  

The effect of exogenous glucose infusion on early embryonic development in lactating dairy cows [PDF]

, 2018
peer-reviewedThe objective of this study was to examine the effect of intravenous infusion of glucose on early embryonic development in lactating dairy cows. Nonpregnant, lactating dairy cows (n = 12) were enrolled in the study (276 ± 17 d in milk). On d
Butler, Stephen, Curran, F., Forde, Niamh, Herlihy, Mary M., Kenneally, J., Leane, S., Lonergan, P., Lucy, Matt C., Simintiras, Constantine A., Sturmey, Roger G.   +9 more
core   +2 more sources

Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Journal of Experimental & Clinical Cancer Research, 2010
Background Real-time reverse transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and sensitivity.
Andersen Solveig N, Sørby Lise, Bukholm Ida RK, Jacobsen Morten B   +3 more
doaj   +1 more source

Genome-Wide Identification and Evaluation of Reference Genes for Quantitative RT-PCR Analysis during Tomato Fruit Development

Frontiers in Plant Science, 2017
Gene expression analysis in tomato fruit has drawn increasing attention nowadays. Quantitative real-time PCR (qPCR) is a routine technique for gene expression analysis.
Yuan Cheng, Wuying Bian, Xin Pang, Jiahong Yu, Golam J. Ahammed, Guozhi Zhou, Rongqing Wang, Meiying Ruan, Zhimiao Li, Qingjing Ye, Zhuping Yao, Yuejian Yang, Hongjian Wan   +12 more
doaj   +1 more source

Reference Genes Selection and Normalization of Oxidative Stress Responsive Genes upon Different Temperature Stress Conditions in Hypericum perforatum L [PDF]

, 2017
Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization ...
Arnholdt-Schmitt, Birgit, Cardoso, Hélia, Ragonezi, Carla, Velada, Isabel   +3 more
core  

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

Frontiers in Physiology
Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica, have been tremendously limited ...
Chen-Hui Shen, Min Tang, Xiao-Fei Li, Li Zhu, Wei Li, Pan Deng, Qing Zhai, Gang Wu, Xiao-Hong Yan   +8 more
doaj   +1 more source

Selection of reference miRNAs for RT-qPCR assays in endometriosis menstrual blood-derived mesenchymal stem cells.

PLoS ONE
Choosing appropriate reference genes or internal controls to normalize RT-qPCR data is mandatory for the interexperimental reproducibility of gene expression data obtained by RT-qPCR in most studies, including those on endometriosis.
Sabrina Yukari Santos Hacimoto, Ana Clara Lagazzi Cressoni, Lilian Eslaine Costa Mendes da Silva, Cristiana Carolina Padovan, Rui Alberto Ferriani, Júlio César Rosa-E-Silva, Juliana Meola   +6 more
doaj   +1 more source

Candidate reference genes for Genotype effect as calculated by the GeNorm, NormFinder and BestKeeper programs.

, 2015
Monteiro Filipa, Sebastiana Mónica, Salomé Pais Maria, Figueiredo Andreia   +3 more
openalex   +2 more sources

The values of geNorm M (A) and geNorm V (B) of 16 reference genes.

, 2015
Zhichen Zhai, Yongchang Yao, Yingjun Wang   +2 more
openalex   +2 more sources