Results 101 to 110 of about 16,155 (172)

Endogenous reference RNAs for microRNA quantitation in formalin-fixed, paraffin-embedded lymph node tissue [PDF]

, 2018
Lymph node metastasis is one of the most important factors for tumor dissemination. Quantifying microRNA (miRNA) expression using real-time PCR in formalin-fixed, paraffin-embedded (FFPE) lymph node can provide valuable information regarding the ...
Cho-Isoda Yukiko, Hori Mitsuo, Iijima Tatsuo, Inada Katsushige, Ishiguro Shingo, Kojima Hiroshi, Oki Akinori, Okoshi Yasushi, Saito Hitoaki, Shimazui Toru, Suzuki Hisashi, Tamaki Yoshio, 大越 靖, 小島 寛, 島居 徹, 沖 明典, 玉木 義雄, 鈴木 久史   +17 more
core   +2 more sources

Evaluation of suitable reference genes for normalization of real-time reverse transcription PCR analysis in colon cancer

Journal of Experimental & Clinical Cancer Research, 2010
Background Real-time reverse transcription PCR (qRT-PCR) is frequently used for gene expression quantification due to its methodological reproducibility and sensitivity.
Andersen Solveig N, Sørby Lise, Bukholm Ida RK, Jacobsen Morten B   +3 more
doaj   +1 more source

Identification of stable reference genes for quantitative PCR in koalas [PDF]

, 2018
To better understand host and immune response to diseases, gene expression studies require identification of reference genes with stable expression for accurate normalisation.
A Kappel, A Radonic, B Artegiani, CA Waugh, CL Andersen, D Kim, DG Ginzinger, F Almeida-Oliveira, F Bartz, G Bamias, GS Meers, GS Simmons, IE Maher, IE Maher, J Vandesompele, J Woinarski, J Yperman, JJ Hanger, K Ahn, KC Thomas, KM Morris, M Kubista, M Pertea, MA Valasek, MW Pfaffl, N Silver, OT Ong, P Brym, PD Lee, PJ Canfield, QL Zhang, R Tarlinton, R Tarlinton, RK Das, S Bages, SA Bustin, SA Bustin, T Burmeister, T Nolan, V Gonzalez-Astudillo, Y Zhang   +40 more
core   +4 more sources

Genome-Wide Identification and Evaluation of Reference Genes for Quantitative RT-PCR Analysis during Tomato Fruit Development

Frontiers in Plant Science, 2017
Gene expression analysis in tomato fruit has drawn increasing attention nowadays. Quantitative real-time PCR (qPCR) is a routine technique for gene expression analysis.
Yuan Cheng, Wuying Bian, Xin Pang, Jiahong Yu, Golam J. Ahammed, Guozhi Zhou, Rongqing Wang, Meiying Ruan, Zhimiao Li, Qingjing Ye, Zhuping Yao, Yuejian Yang, Hongjian Wan   +12 more
doaj   +1 more source

Evaluation of reference genes for quantitative expression analysis in Mylabris sibirica (Coleoptera, Meloidae)

Frontiers in Physiology
Mylabris sibirica is a hypermetamorphic insect whose adults feed on oilseed rape. However, due to a shortage of effective and appropriate endogenous references, studies on molecular functional genes in Mylabris sibirica, have been tremendously limited ...
Chen-Hui Shen, Min Tang, Xiao-Fei Li, Li Zhu, Wei Li, Pan Deng, Qing Zhai, Gang Wu, Xiao-Hong Yan   +8 more
doaj   +1 more source

Endogenous reference genes for gene expression studies on bicuspid aortic valve associated aortopathy in humans

, 2016
Bicuspid aortic valve (BAV) disease is the most common congenital cardiac abnormality and predisposes patients to life-threatening aortic complications including aortic aneurysm.
Cagampang, Felino, Harrison, Oliver, Moorjani, Narain, Ohri, Sunil, Torrens, Christopher   +4 more
core   +3 more sources

Identifying the most suitable endogenous control for determining gene expression in hearts from organ donors [PDF]

, 2013
BACKGROUND: Quantitative real-time reverse transcription PCR (qRT-PCR) is a useful tool for assessing gene expression in different tissues, but the choice of adequate controls is critical to normalise the results, thereby avoiding differences and ...
Astudillo, Aurora, Escudero, Dolores, Gómez, Enrique, Otero, Jesús, Pérez, Silvia, Rodríguez, Aida, Royo, L.J. (Luis), Álvarez, Francisco   +7 more
core  

Selection of reference miRNAs for RT-qPCR assays in endometriosis menstrual blood-derived mesenchymal stem cells.

PLoS ONE
Choosing appropriate reference genes or internal controls to normalize RT-qPCR data is mandatory for the interexperimental reproducibility of gene expression data obtained by RT-qPCR in most studies, including those on endometriosis.
Sabrina Yukari Santos Hacimoto, Ana Clara Lagazzi Cressoni, Lilian Eslaine Costa Mendes da Silva, Cristiana Carolina Padovan, Rui Alberto Ferriani, Júlio César Rosa-E-Silva, Juliana Meola   +6 more
doaj   +1 more source

Reference Genes Selection and Normalization of Oxidative Stress Responsive Genes upon Different Temperature Stress Conditions in Hypericum perforatum L [PDF]

, 2017
Reverse transcription-quantitative real-time PCR (RT-qPCR) is a widely used technique for gene expression analysis. The reliability of this method depends largely on the suitable selection of stable reference genes for accurate data normalization ...
Arnholdt-Schmitt, Birgit, Cardoso, Hélia, Ragonezi, Carla, Velada, Isabel   +3 more
core  

Identification and validation of quantitative PCR reference genes suitable for normalising expression in normal and dystrophic cell culture models of myogenesis [PDF]

, 2014
Hildyard, J C W, Wells, D J
core   +1 more source