Results 161 to 170 of about 16,155 (172)
Identification and Validation of Tissue-Specific Housekeeping Markers for the Amazon River Prawn Macrobrachium amazonicum (Heller, 1862). [PDF]
Genes (Basel)Lima GM +11 more
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Selection of stable reference genes for accurate reverse-transcription quantitative PCR in cotton-herbivore studies using virus-induced gene silencing. [PDF]
Sci RepClark MK, Behmer ST, Sword GA.
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Evaluation of candidate RT-qPCR reference genes in the aging African turquoise killifish brain. [PDF]
Aging BrainWhisenant E, Lekven AC.
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Fish & Shellfish Immunology, 2011
Megalobrama amblycephala suffers from serious liver diseases recently and PPARα gene has been reported to play an important role in the immune system of animal liver. On the basis of these facts, we have cloned and identified full-length cDNA of PPARα and examined its expression patterns at different embryo developmental stages and in different tissues
Yuhua, Zhao +3 more
openaire +2 more sources
Megalobrama amblycephala suffers from serious liver diseases recently and PPARα gene has been reported to play an important role in the immune system of animal liver. On the basis of these facts, we have cloned and identified full-length cDNA of PPARα and examined its expression patterns at different embryo developmental stages and in different tissues
Yuhua, Zhao +3 more
openaire +2 more sources
Electromagnetic Biology and Medicine, 2011
Studies of animals and humans subjected to spaceflight demonstrate that weightlessness negatively affects the mass and mechanical properties of bone tissue. Bone cells could sense and respond to the gravity unloading, and genes sensitive to gravity change were considered to play a critical role in the mechanotransduction of bone cells.
S. Di +6 more
openaire +2 more sources
Studies of animals and humans subjected to spaceflight demonstrate that weightlessness negatively affects the mass and mechanical properties of bone tissue. Bone cells could sense and respond to the gravity unloading, and genes sensitive to gravity change were considered to play a critical role in the mechanotransduction of bone cells.
S. Di +6 more
openaire +2 more sources
Gene, 2013
In order to be able to modulate and improve the function of PPARγ and decrease further some metabolic diseases of M. amblycephala, we have cloned and identified the full-length cDNA of PPARγ in M. amblycephala and examined its transcription patterns at different embryo developmental stages and in different tissues of adult and immature fish.
Su, Li +4 more
openaire +2 more sources
In order to be able to modulate and improve the function of PPARγ and decrease further some metabolic diseases of M. amblycephala, we have cloned and identified the full-length cDNA of PPARγ in M. amblycephala and examined its transcription patterns at different embryo developmental stages and in different tissues of adult and immature fish.
Su, Li +4 more
openaire +2 more sources
International Journal of Legal Medicine, 2012
In forensic molecular pathology, quantitative real-time polymerase chain reaction (RT-qPCR) provides a rapid and sensitive method to investigate functional changes in the death process. Accurate and reliable relative RT-qPCR requires ideal amplification efficiencies of target and reference genes.
Qi, Wang +5 more
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In forensic molecular pathology, quantitative real-time polymerase chain reaction (RT-qPCR) provides a rapid and sensitive method to investigate functional changes in the death process. Accurate and reliable relative RT-qPCR requires ideal amplification efficiencies of target and reference genes.
Qi, Wang +5 more
openaire +2 more sources
Veterinary Immunology and Immunopathology, 2009
Quantitative real time PCR (Q-PCR) is the method of choice to study mRNA expression levels. Since Q-PCR is very sensitive, normalization of the data with stably expressed reference genes if of utmost importance. The stability of reference genes depends on the tissue and the species of interest.
Schlotter, Yvette M. +6 more
openaire +4 more sources
Quantitative real time PCR (Q-PCR) is the method of choice to study mRNA expression levels. Since Q-PCR is very sensitive, normalization of the data with stably expressed reference genes if of utmost importance. The stability of reference genes depends on the tissue and the species of interest.
Schlotter, Yvette M. +6 more
openaire +4 more sources
Journal of Clinical Oncology, 2006
20052 Background: Robust quantitation of potential clinical marker genes using quantitative real-time PCR (Q RT-PCR) is critically dependent on accurate normalization. Although GAPDH has historically been used for normalization, its expression has been shown to vary widely between different tissues and experimental conditions.
G. Eick +6 more
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20052 Background: Robust quantitation of potential clinical marker genes using quantitative real-time PCR (Q RT-PCR) is critically dependent on accurate normalization. Although GAPDH has historically been used for normalization, its expression has been shown to vary widely between different tissues and experimental conditions.
G. Eick +6 more
openaire +1 more source
2013
The quantification of mRNAs has been used with great success in many medical research techniques. All of them can use housekeeping genes as internal standards. While most of the commonly used housekeeping genes may have varied expression stability in different human tissue samples or experimental conditions.
Li Li +3 more
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The quantification of mRNAs has been used with great success in many medical research techniques. All of them can use housekeeping genes as internal standards. While most of the commonly used housekeeping genes may have varied expression stability in different human tissue samples or experimental conditions.
Li Li +3 more
openaire +1 more source