Results 181 to 190 of about 16,273 (193)

Reference Gene Selection for Quantitative Gene Expression Analysis in <i>Argynnis hyperbius</i>. [PDF]

Insects
Xin HJ, Liu CY, Yan F, Wang LD, Zhang HH, Shen CH, Zhai Q.   +6 more
europepmc   +1 more source

Selection and validation of stable reference genes in potato infected by Pectobacterium atrosepticum using real-time quantitative PCR. [PDF]

Sci Rep
Hou L, Zhu H, Xian W, Ma Y.
europepmc   +1 more source

GENORM: A generalized norm calculation

Computers & Geosciences, 1991
Abstract This program provides a way to calculate a norm composed of any group of minerals selected by the user. The norm minerals may comprise the CIPW set, but also may include any other minerals defined by formula or chemical analysis. The standard CIPW norm calculation minimizes the sum of norm minerals when calculated as molecular amounts not ...
K L Currie
openaire   +2 more sources

Cloning, identification and accurate normalization expression analysis of PPARα gene by GeNorm in Megalobrama amblycephala

Fish & Shellfish Immunology, 2011
Megalobrama amblycephala suffers from serious liver diseases recently and PPARα gene has been reported to play an important role in the immune system of animal liver. On the basis of these facts, we have cloned and identified full-length cDNA of PPARα and examined its expression patterns at different embryo developmental stages and in different tissues
Yuhua, Zhao, Yasmeen, Gul, Su, Li, Weimin, Wang   +3 more
openaire   +3 more sources

Selection of suitable reference genes from bone cells in large gradient high magnetic field based on GeNorm algorithm

Electromagnetic Biology and Medicine, 2011
Studies of animals and humans subjected to spaceflight demonstrate that weightlessness negatively affects the mass and mechanical properties of bone tissue. Bone cells could sense and respond to the gravity unloading, and genes sensitive to gravity change were considered to play a critical role in the mechanotransduction of bone cells.
S. Di, Z. Tian, A. Qian, X. Gao, D. Yu, BRANDI, MARIA LUISA, P. Shang   +6 more
openaire   +3 more sources

PPARγ, an important gene related to lipid metabolism and immunity in Megalobrama amblycephala: Cloning, characterization and transcription analysis by GeNorm

Gene, 2013
In order to be able to modulate and improve the function of PPARγ and decrease further some metabolic diseases of M. amblycephala, we have cloned and identified the full-length cDNA of PPARγ in M. amblycephala and examined its transcription patterns at different embryo developmental stages and in different tissues of adult and immature fish.
Su, Li, Yasmeen, Gul, Weimin, Wang, Xueqiao, Qian, Yuhua, Zhao   +4 more
openaire   +3 more sources

geNorm assessment of the efficacy of novel reference genes ALG9, TFCP2 and ZNF410 compared to GAPDH for real time normalization in GI carcinoids

Journal of Clinical Oncology, 2006
20052 Background: Robust quantitation of potential clinical marker genes using quantitative real-time PCR (Q RT-PCR) is critically dependent on accurate normalization. Although GAPDH has historically been used for normalization, its expression has been shown to vary widely between different tissues and experimental conditions.
G. Eick, M. Kidd, S. Mane, B. Nadler, M. Champaneria, R. Pfragner, I. M. Modlin   +6 more
openaire   +2 more sources

A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis

Veterinary Immunology and Immunopathology, 2009
Quantitative real time PCR (Q-PCR) is the method of choice to study mRNA expression levels. Since Q-PCR is very sensitive, normalization of the data with stably expressed reference genes if of utmost importance. The stability of reference genes depends on the tissue and the species of interest.
Schlotter, Yvette M., Veenhof, Eveline Z., Brinkhof, Bas, Rutten, Victor P.M.G., Spee, Bart, Willemse, Ton, Penning, Louis C.   +6 more
openaire   +5 more sources

Sample-Independent Expression Stability Analysis of Human Housekeeping Genes Using the GeNORM Algorithm

, 2013
The quantification of mRNAs has been used with great success in many medical research techniques. All of them can use housekeeping genes as internal standards. While most of the commonly used housekeeping genes may have varied expression stability in different human tissue samples or experimental conditions.
Li Li, Xiaofang Mao, Qiang Gao, Yicheng Cao   +3 more
openaire   +2 more sources

Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper

International Journal of Legal Medicine, 2012
In forensic molecular pathology, quantitative real-time polymerase chain reaction (RT-qPCR) provides a rapid and sensitive method to investigate functional changes in the death process. Accurate and reliable relative RT-qPCR requires ideal amplification efficiencies of target and reference genes.
Qi, Wang, Takaki, Ishikawa, Tomomi, Michiue, Bao-Li, Zhu, Da-Wei, Guan, Hitoshi, Maeda   +5 more
openaire   +3 more sources